RESUMEN
PURPOSE: To investigate the effect of different light-curing units and depths on the degree of conversion (DC) through Fourier transform infrared spectroscopy (FTIR) and Knoop Hardness Number (KHN) of a silorane-based composite resin (Filtek LS, 3M ESPE, St Paul, MN, USA) (LS). MATERIALS AND METHODS: LS specimens mounted in a particular designed matrix were photoactivated by three light-cure units (LCUs) at depths of 2, 3, 4, and 5 mm. The DC was determined in a FTIR spectrometer with an attenuated total reflectance accessory. The KHN was measured in an automatic microhardness tester. The results were analyzed using the Friedman and Spearman statistical tests (α=0.05). RESULTS: There was no effect of LCUs on the DC (p=0.472) or KHN (p=0.174) for all of the studied depths. The highest DC and KHN means were found at 2-mm depth, which were not statistically different from 3-mm depth, but were higher than 4-mm and 5-mm depths (p=0.007). Spearman analysis found a positive linear correlation between the variables KHN and DC (r=0.858, p<0.000). CONCLUSIONS: The LCUs' effect was not verified. Values of DC and KHN for LS decreased with increasing depth. The highest values for both DC and KHN were obtained at depths of 2-3 mm.
Asunto(s)
Resinas Compuestas/uso terapéutico , Curación por Luz de Adhesivos Dentales/métodos , Restauración Dental Permanente/métodos , Análisis del Estrés Dental , Dureza , Humanos , Espectroscopía Infrarroja por Transformada de FourierRESUMEN
AIM: To investigate the possible association between TNF-alpha (-308G/A) polymorphism and toxoplasmic retinochoroiditis (TR) in humans. METHODS: A cross-sectional study was performed which included 100 Brazilian patients with diagnosis of TR and 100 matched control subjects with positive serology to toxoplasmosis and no sign of uveitis. Genomic DNA was obtained from oral swabs of all subjects and amplified using the polymerase chain reaction (PCR) with specific primers flanking the locus -308 of TNF-alpha. PCR products were submitted to restriction endonuclease digestion and analysed by polyacrylamide gel electrophoresis to distinguish alleles, allowing the determination of the genotypes. RESULTS: There was no significant difference in the genotype (chi(2) = 0.79, p = 0.67), allele (chi(2) = 0.095, p = 0.75) and allele carriage (chi(2) = 0.70, p = 0.40) frequencies in TR patients compared with control subjects. Frequencies of the genotype (chi(2) = 2.05, p = 0.35) and allele (chi(2) = 0.13, p = 0.71) did not differ significantly between TR patients with and without recurrent episodes. CONCLUSION: This is the first study to investigate the association between TNF-alpha polymorphism and the occurrence of TR in humans. TNF-alpha gene polymorphism (-308G/A) does not seem to be associated with the occurrence or recurrence of TR.
Asunto(s)
Coriorretinitis/genética , Polimorfismo Genético , Toxoplasmosis Ocular/genética , Factor de Necrosis Tumoral alfa/genética , Adulto , Estudios de Casos y Controles , Coriorretinitis/parasitología , Coriorretinitis/fisiopatología , Estudios Transversales , Electroforesis en Gel de Poliacrilamida/métodos , Femenino , Frecuencia de los Genes , Predisposición Genética a la Enfermedad , Humanos , Masculino , Persona de Mediana Edad , Reacción en Cadena de la Polimerasa/métodos , Toxoplasmosis Ocular/fisiopatología , Agudeza VisualAsunto(s)
Aspergilosis/diagnóstico , Endoftalmitis/microbiología , Huésped Inmunocomprometido , Leucemia Linfocítica Crónica de Células B/microbiología , Antifúngicos/uso terapéutico , Aspergilosis/tratamiento farmacológico , Endoftalmitis/tratamiento farmacológico , Fondo de Ojo , Humanos , Leucemia Linfocítica Crónica de Células B/tratamiento farmacológico , Masculino , Persona de Mediana EdadRESUMEN
In this study, 997 leprosy patients were examined, 528 of them with lepromatous leprosy (53%), 199 with borderline leprosy (20%), 167 with tuberculoid leprosy (16%) and 103 (10.3%) with indeterminate leprosy. Changes in the ocular bulb were noted in 314 patients (31.5%) specially in those with lepromatous leprosy. These alterations were greater with increasing age of the patient and length of disease. Severe ocular lesions were rare, probably due to previous systemic treatment. The "pearls" in the fundus of the eye resulting from leprosy were also studied.
Asunto(s)
Oftalmopatías/epidemiología , Oftalmopatías/etiología , Lepra/complicaciones , Lepra/epidemiología , Trastornos de la Visión/epidemiología , Trastornos de la Visión/etiología , Adolescente , Adulto , Anciano , Anciano de 80 o más Años , Brasil/epidemiología , Niño , Preescolar , Técnicas de Diagnóstico Oftalmológico , Ojo/patología , Oftalmopatías/diagnóstico , Oftalmopatías/patología , Oftalmopatías/terapia , Femenino , Humanos , Masculino , Persona de Mediana Edad , Trastornos de la Visión/diagnóstico , Trastornos de la Visión/patología , Trastornos de la Visión/terapia , Agudeza VisualRESUMEN
The authors conducted an anterior chamber paracentesis in a patient with lepromatous leprosy showing bilateral iridocyclitis. The paracentesis was performed in the outpatients department. The aqueous humor was studied by Ziehl-Nielsen staining method and the result was the isolation of the M. leprae in the anterior chamber. This study shows that M. leprae can promote uveitis in leprosy patients. Therefore, it should be looked for in patients having this type of disease.
Asunto(s)
Infecciones Bacterianas del Ojo/microbiología , Iridociclitis/microbiología , Lepra Lepromatosa/microbiología , Mycobacterium leprae/aislamiento & purificación , Paracentesis , Anciano , Cámara Anterior/microbiología , Humor Acuoso/microbiología , Femenino , HumanosRESUMEN
The technique of superior tarsal conjunctiva scrape was used for identifying M.leprae in the conjunctiva in 56 leprosy patients (all of them multibacillary, some untreated and others treated with multidrug therapy). The technique of tarsal conjunctiva scrape was shown to be more suitable than conjunctival biopsy for identifying lepra bacilli. This technique is also easier to perform and has shown a statistical relation between bacilloscopical index of skin (BIsk) and bacilloscopical index of tarsal conjunctiva (BIconj) values. Thus, if the bacilli can be identified at tarsal conjunctiva we can assume greater systemic bacillary load in the patients.