RESUMEN
Säo descritas as principais etapas de uma técnica simples e sensível para a pesquisa, na mesma placa, do antígeno, do anticorpo e de imunocomplexos específicos baseada no "Enzyme-Linked Immunosorbent Assay" (ELISA). A técnica se mostrou de grande utilidade na pesquisa do antígeno, do anticorpo e de imunocomplexos específicos no soro de pacientes com esquistossomose hépato-intestinal crônica
Asunto(s)
Niño , Adolescente , Adulto , Humanos , Masculino , Femenino , Complejo Antígeno-Anticuerpo/análisis , Antígenos Helmínticos/análisis , Schistosoma mansoni/inmunología , Esquistosomiasis mansoni/inmunología , Técnicas para InmunoenzimasRESUMEN
The main steps of a sensitive and quantitative technique for the simultaneous determination, in the same microplate, of antigen, antibody and specific immune complexes has been described. The assay involved principles of the double sandwich technique used in the "Enzyme-Linked Immunosorbent Assay" (ELISA). The technique proved to be useful for the estimation of antigen, antibody and specific immune complexes in sera of patients with human chronic hepato-intestinal schistosomiasis.
Asunto(s)
Anticuerpos/análisis , Complejo Antígeno-Anticuerpo/análisis , Antígenos Helmínticos/análisis , Schistosoma mansoni/inmunología , Esquistosomiasis mansoni/inmunología , Adolescente , Adulto , Niño , Femenino , Humanos , Técnicas para Inmunoenzimas , MasculinoAsunto(s)
Lactante , Preescolar , Humanos , Masculino , Femenino , Inmunoglobulinas , Desnutrición Proteico-CalóricaAsunto(s)
Adulto , Persona de Mediana Edad , Humanos , Masculino , Femenino , Ritmo Circadiano , Hidrocortisona , Úlcera PépticaRESUMEN
Attempts at restoring the state of malnutrition experimentally induced in rats during pre- and post-natal periods, led to the following remarks: 1) animals fed a normal diet soon improved their weight and levels of this blood proteins; 2) the restoration levels obtained at the end of 120 days of treatment did not reach, however, that obtained by the control animals.