RESUMEN
We established a low background, Cre-dependent version of the inducible Tet-On system for fast, cell type-specific transgene expression in vivo Coexpression of a constitutive, Cre-dependent fluorescent marker selectively allowed single-cell analyses before and after inducible, Tet-dependent transgene expression. Here, we used this method for precise, acute manipulation of neuronal activity in the living brain. The goal was to study neuronal network homeostasis at cellular resolution. Single induction of the potassium channel Kir2.1 produced cell type-specific silencing within hours that lasted for at least 3 d. Longitudinal in vivo imaging of spontaneous calcium transients and neuronal morphology demonstrated that prolonged silencing did not alter spine densities or synaptic input strength. Furthermore, selective induction of Kir2.1 in parvalbumin interneurons increased the activity of surrounding neurons in a distance-dependent manner. This high-resolution, inducible interference and interval imaging of individual cells (high I5, HighFive) method thus allows visualizing temporally precise, genetic perturbations of defined cells.SIGNIFICANCE STATEMENT Gene function is studied by KO or overexpression of a specific gene followed by analyses of phenotypic changes. However, being able to predict and analyze exactly those cells in which genetic manipulation will occur is not possible. We combined two prominent transgene overexpression methods to fluorescently highlight the targeted cells appropriately before cell type-specific transgene induction. By inducing a potassium channel that decreases neuronal firing, we investigated how neuronal networks in the living mouse brain possibly compensate swift changes in cellular activities. Unlike in vitro, known compensatory homeostatic mechanisms, such as changes in synapses, were not observed in vivo Overall, we demonstrated with our method rapid genetic manipulation and analysis of neuronal activities as well as precision transgene expression.
Asunto(s)
Interneuronas , Neuronas , Ratones , Animales , Neuronas/fisiología , Transgenes , Homeostasis/fisiología , Canales de Potasio/metabolismoRESUMEN
Alcohol intoxication at early ages is a risk factor for the development of addictive behavior. To uncover neuronal molecular correlates of acute ethanol intoxication, we used stable-isotope-labeled mice combined with quantitative mass spectrometry to screen more than 2,000 hippocampal proteins, of which 72 changed synaptic abundance up to twofold after ethanol exposure. Among those were mitochondrial proteins and proteins important for neuronal morphology, including MAP6 and ankyrin-G. Based on these candidate proteins, we found acute and lasting molecular, cellular, and behavioral changes following a single intoxication in alcohol-naïve mice. Immunofluorescence analysis revealed a shortening of axon initial segments. Longitudinal two-photon in vivo imaging showed increased synaptic dynamics and mitochondrial trafficking in axons. Knockdown of mitochondrial trafficking in dopaminergic neurons abolished conditioned alcohol preference in Drosophila flies. This study introduces mitochondrial trafficking as a process implicated in reward learning and highlights the potential of high-resolution proteomics to identify cellular mechanisms relevant for addictive behavior.
Asunto(s)
Intoxicación Alcohólica , Neuronas Dopaminérgicas , Etanol , Hipocampo , Proteínas del Tejido Nervioso , Intoxicación Alcohólica/metabolismo , Intoxicación Alcohólica/patología , Animales , Conducta Adictiva/inducido químicamente , Neuronas Dopaminérgicas/efectos de los fármacos , Neuronas Dopaminérgicas/metabolismo , Relación Dosis-Respuesta a Droga , Drosophila melanogaster , Etanol/administración & dosificación , Etanol/toxicidad , Técnicas de Silenciamiento del Gen , Hipocampo/efectos de los fármacos , Hipocampo/metabolismo , Ratones , Mitocondrias/metabolismo , Proteínas del Tejido Nervioso/genética , Proteínas del Tejido Nervioso/metabolismo , Transporte de Proteínas/efectos de los fármacosRESUMEN
BACKGROUND: Little is known why proteins and RNAs exhibit half-lives varying over several magnitudes. Despite many efforts, a conclusive link between half-lives and gene function could not be established suggesting that other determinants may influence these molecular attributes. RESULTS: Here, I find that with increasing gene age there is a gradual and significant increase of protein and RNA half-lives, protein structure, and other molecular attributes that tend to affect protein abundance. These observations are accommodated in a hypothesis which posits that new genes at 'birth' are not optimized and thus their products exhibit low half-lives and less structure but continuous mutagenesis eventually improves these attributes. Thus, the protein and RNA products of the oldest genes obtained their high degrees of stability and structure only after billions of years while the products of younger genes had less time to be optimized and are therefore less stable and structured. Because more stable proteins with lower turnover require less transcription to maintain the same level of abundance, reduced transcription-associated mutagenesis (TAM) would fixate the changes by increasing gene conservation. CONCLUSIONS: Consequently, the currently observed diversity of molecular attributes is a snapshot of gene products being at different stages along their temporal path of optimization.
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Biología Computacional , Proteínas/genética , Proteínas/metabolismo , ARN/genética , ARN/metabolismo , Animales , Semivida , Células HeLa , Humanos , Ratones , Mutagénesis , Especificidad de la Especie , Transcripción GenéticaRESUMEN
Recent evidence suggests that the extracellular protein milieu is much more complex than previously assumed as various secretome analyses from different cell types described the release of hundreds to thousands of proteins. The extracellular function of many of these proteins has yet to be determined particularly in the context of three-dimensional tissues with abundant cell-cell contacts. Toward this goal, we developed a strategy of dual SILAC labeling astrocytic cultures for in silico exclusion of unlabeled proteins from serum or neurons used for stimulation. For constitutive secretion, this strategy allowed the precise quantification of the extra-to-intracellular protein ratio of more than 2000 identified proteins. Ratios covered 4 orders of magnitude indicating that the intracellular vs extracellular contributions of different proteins can be variable. Functionally, the secretome of labeled forebrain astrocytic cultures specifically changed within hours after adding unlabeled, "physiological" forebrain neurons. "Nonphysiological" cerebellar hindbrain neurons, however, elicited a different, highly repulsive secretory response. Our data also suggest a significant association of constitutive secretion with the classical secretion pathway and regulated secretion with unconventional pathways. We conclude that quantitative proteomics can help to elucidate general principles of cellular secretion and provide functional insight into the abundant extracellular presence of proteins.
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Aminoácidos/metabolismo , Comunicación Celular , Proteoma/metabolismo , Proteómica/métodos , Animales , Astrocitos/citología , Astrocitos/metabolismo , Western Blotting , Células Cultivadas , Marcaje Isotópico/métodos , Espectrometría de Masas , Neuroglía/citología , Neuroglía/metabolismo , Neuronas/citología , Neuronas/metabolismo , Prosencéfalo/citología , Ratas , Transducción de SeñalRESUMEN
Neuronal processing is classically conceptualized as dendritic input, somatic integration, and axonal output. The axon initial segment, the proposed site of action potential generation, usually emanates directly from the soma. However, we found that axons of hippocampal pyramidal cells frequently derive from a basal dendrite rather than from the soma. This morphology is particularly enriched in central CA1, the principal hippocampal output area. Multiphoton glutamate uncaging revealed that input onto the axon-carrying dendrites (AcDs) was more efficient in eliciting action potential output than input onto regular basal dendrites. First, synaptic input onto AcDs generates action potentials with lower activation thresholds compared with regular dendrites. Second, AcDs are intrinsically more excitable, generating dendritic spikes with higher probability and greater strength. Thus, axon-carrying dendrites constitute a privileged channel for excitatory synaptic input in a subset of cortical pyramidal cells.
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Axones/fisiología , Dendritas/fisiología , Hipocampo/fisiología , Células Piramidales/fisiología , Transmisión Sináptica/fisiología , Potenciales de Acción/fisiología , Animales , Axones/ultraestructura , Simulación por Computador , Dendritas/ultraestructura , Potenciales Postsinápticos Excitadores/fisiología , Femenino , Hipocampo/ultraestructura , Inmunohistoquímica , Masculino , Ratones , Ratones Endogámicos C57BL , Ratones Transgénicos , Microscopía Confocal , Modelos Neurológicos , Técnicas de Cultivo de Órganos , Técnicas de Placa-Clamp , Células Piramidales/ultraestructura , Ratas , Ratas WistarRESUMEN
Ocular dominance (OD) columns, alternating regions of left and right eye input in the visual cortex of higher mammals, have long been thought to develop from an initially intermingled state by an activity-dependent process. While indirect evidence points to potential alternative mechanisms based on molecular cues, direct proof for a molecular difference between left- and right eye columns is missing. Here, we show that heat shock protein 90 alpha (Hsp90α) is expressed in a clustered fashion in the developing cat visual cortex. Clusters of Hsp90α-positive cells are in register with OD columns of the ipsilateral eye as early as postnatal day 16, when OD columns have just appeared. Importantly, a periodic, clustered expression of Hsp90α is already present weeks before OD columns have started to form, suggesting that molecular differences between future left and right eye OD columns may contribute to the segregated termination of eye specific afferents in the developing visual cortex.
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Predominio Ocular/fisiología , Proteínas HSP90 de Choque Térmico/metabolismo , Corteza Visual/crecimiento & desarrollo , Animales , Gatos , Corteza Visual/metabolismo , Percepción Visual/fisiologíaRESUMEN
The turnover of each protein in the mammalian proteome is a functionally important characteristic. Here, we employed high-resolution mass spectrometry to quantify protein dynamics in nondividing mammalian cells. The ratio of externally supplied versus endogenous amino acids to de novo protein synthesis was about 17:1. Using subsaturating SILAC labeling, we obtained accurate turnover rates of 4106 proteins in HeLa and 3528 proteins in C2C12 cells. Comparison of these human and mouse cell lines revealed a highly significant turnover correlation of protein orthologs and thus high species conservation. Functionally, we observed statistically significant trends for the turnover of phosphoproteins and gene ontology categories that showed extensive covariation between mouse and human. Likewise, the members of some protein complexes, such as the proteasome, have highly similar turnover rates. The high species conservation and the low complex variances thus imply great regulatory fine-tuning of protein turnover.
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Biosíntesis de Proteínas , Proteoma/análisis , Proteómica/métodos , Secuencia de Aminoácidos , Aminoácidos/metabolismo , Animales , Puntos de Control del Ciclo Celular , Biología Computacional , Secuencia Conservada , Semivida , Células HeLa , Humanos , Marcaje Isotópico , Espectrometría de Masas , Ratones , Datos de Secuencia Molecular , Péptidos/metabolismo , Fosforilación , Proteolisis , Proteoma/metabolismo , Especificidad de la EspecieRESUMEN
High spatial and temporal resolution of conditional gene expression is typically difficult to achieve in whole tissues or organisms. We synthesized two reversibly inhibited, photoactivatable ('caged') doxycycline derivatives with different membrane permeabilities for precise spatial and temporal light-controlled activation of transgenes based on the 'Tet-on' system. After incubation with caged doxycycline or caged cyanodoxycycline, we induced gene expression by local irradiation with UV light or by two-photon uncaging in diverse biological systems, including mouse organotypic brain cultures, developing mouse embryos and Xenopus laevis tadpoles. The amount of UV light needed for induction was harmless as we detected no signs of toxicity. This method allows high-resolution conditional transgene expression at different spatial scales, ranging from single cells to entire complex organisms.
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Doxiciclina/farmacología , Expresión Génica/efectos de los fármacos , Expresión Génica/efectos de la radiación , Animales , Animales Modificados Genéticamente , Doxiciclina/análogos & derivados , Doxiciclina/química , Técnicas de Cultivo de Embriones , Desarrollo Embrionario/efectos de los fármacos , Desarrollo Embrionario/genética , Desarrollo Embrionario/efectos de la radiación , Femenino , Proteínas Fluorescentes Verdes/genética , Hipocampo/efectos de los fármacos , Hipocampo/metabolismo , Hipocampo/efectos de la radiación , Larva/efectos de los fármacos , Larva/genética , Larva/efectos de la radiación , Ratones , Ratones Transgénicos , Microscopía de Fluorescencia por Excitación Multifotónica , Fotobiología , Embarazo , Proteínas Recombinantes/genética , Técnicas de Cultivo de Tejidos , Rayos Ultravioleta , Xenopus laevis/genética , Xenopus laevis/crecimiento & desarrolloAsunto(s)
Doxiciclina/análogos & derivados , Regulación de la Expresión Génica/efectos de la radiación , Fotoquímica , Rayos Ultravioleta , Animales , Células CHO/efectos de la radiación , Cricetinae , Cricetulus , Doxiciclina/farmacología , Glucuronidasa/genética , Glucuronidasa/metabolismo , Glucuronidasa/efectos de la radiación , Proteínas Fluorescentes Verdes/genética , Proteínas Fluorescentes Verdes/metabolismo , Proteínas Fluorescentes Verdes/efectos de la radiaciónRESUMEN
Dendritic spines on pyramidal neurons receive the vast majority of excitatory input and are considered electrobiochemical processing units, integrating and compartmentalizing synaptic input. Following synaptic plasticity, spines can undergo morphological plasticity, which possibly forms the structural basis for long-term changes in neuronal circuitry. Here, we demonstrate that spines on CA1 pyramidal neurons from organotypic slice cultures show bidirectional activity-dependent morphological plasticity. Using two-photon time-lapse microscopy, we observed that low-frequency stimulation induced NMDA receptor-dependent spine retractions, whereas theta burst stimulation led to the formation of new spines. Moreover, without stimulation the number of spine retractions was on the same order of magnitude as the stimulus-induced spine gain or loss. Finally, we found that the ability of neurons to eliminate spines in an activity-dependent manner decreased with developmental age. Taken together, our data show that hippocampal neurons can undergo bidirectional morphological plasticity; spines are formed and eliminated in an activity-dependent way.