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1.
Front Plant Sci ; 14: 1324152, 2023.
Artículo en Inglés | MEDLINE | ID: mdl-38034568

RESUMEN

Introduction: Nondestructive detection of thin-skinned fruit bruising is one of the main challenges in the automated grading of post-harvest fruit. The structured-illumination reflectance imaging (SIRI) is an emerging optical technique with the potential for detection of bruises. Methods: This study presented the pioneering application of low-cost visible-LED SIRI for detecting early subcutaneous bruises in 'Korla' pears. Three types of bruising degrees (mild, moderate and severe) and ten sets of spatial frequencies (50, 100, 150, 200, 250, 300, 350, 400, 450 and 500 cycles m-1) were analyzed. By evaluation of contrast index (CI) values, 150 cycles m-1 was determined as the optimal spatial frequency. The sinusoidal pattern images were demodulated to get the DC, AC, and RT images without any stripe information. Based on AC and RT images, texture features were extracted and the LS-SVM, PLS-DA and KNN classification models combined the optimized features were developed for the detection of 'Korla' pears with varying degrees of bruising. Results and discussion: It was found that RT images consistently outperformed AC images regardless of type of model, and LS-SVM model exhibited the highest detection accuracy and stability. Across mild, moderate, severe and mixed bruises, the LS-SVM model with RT images achieved classification accuracies of 98.6%, 98.9%, 98.5%, and 98.8%, respectively. This study showed that visible-LED SIRI technique could effectively detect early bruising of 'Korla' pears, providing a valuable reference for using low-cost visible LED SIRI to detect fruit damage.

2.
Front Plant Sci ; 13: 952942, 2022.
Artículo en Inglés | MEDLINE | ID: mdl-36035725

RESUMEN

Citrus fruits are susceptible to fungal infection after harvest. To reduce the economic loss, it is necessary to reject the infected citrus fruit before storage and transportation. However, the infected area in the early stage of decay is almost invisible on the fruit surface, so the detection of early decayed citrus is very challenging. In this study, a structured-illumination reflectance imaging (SIRI) system combined with a visible light-emitting diode (LED) lamp and a monochrome camera was developed to detect early fungal infection in oranges. Under sinusoidal modulation illumination with spatial frequencies of 0.05, 0.15, and 0.25 cycles mm-1, three-phase-shifted images with phase offsets of - 2π/3, 0, and 2π/3 were acquired for each spatial frequency. The direct component (DC) and alternating component (AC) images were then recovered by image demodulation using a three-phase-shifting approach. Compared with the DC image, the decayed area can be clearly identified in the AC image and RT image (AC/DC). The optimal spatial frequency was determined by analyzing the AC image and pixel intensity distribution. Based on the texture features extracted from DC, AC, and RT images, four kinds of classification models including partial least square discriminant analysis (PLS-DA), support vector machine (SVM), least squares-support vector machine (LS-SVM), and k-nearest neighbor (KNN) were established to detect the infected oranges, respectively. Model optimization was also performed by extracting important texture features. Compared to all models, the PLS-DA model developed based on eight texture features of RT images achieved the optimal classification accuracy of 96.4%. This study showed for the first time that the proposed SIRI system combined with appropriate texture features and classification model can realize the early detection of decayed oranges.

3.
Foods ; 10(12)2021 Dec 03.
Artículo en Inglés | MEDLINE | ID: mdl-34945536

RESUMEN

Watercore is an internal physiological disorder affecting the quality and price of apples. Rapid and non-destructive detection of watercore is of great significance to improve the commercial value of apples. In this study, the visible and near infrared (Vis/NIR) full-transmittance spectroscopy combined with analysis of variance (ANOVA) method was used for online detection of watercore apples. At the speed of 0.5 m/s, the effects of three different orientations (O1, O2, and O3) on the discrimination results of watercore apples were evaluated, respectively. It was found that O3 orientation was the most suitable for detecting watercore apples. One-way ANOVA was used to select the characteristic wavelengths. The least squares-support vector machine (LS-SVM) model with two characteristic wavelengths obtained good performance with the success rates of 96.87% and 100% for watercore and healthy apples, respectively. In addition, full-spectrum data was also utilized to determine the optimal two-band ratio for the discrimination of watercore apples by ANOVA method. Study showed that the threshold discrimination model established based on O3 orientation had the same detection accuracy as the optimal LS-SVM model for samples in the prediction set. Overall, full-transmittance spectroscopy combined with the ANOVA method was feasible to online detect watercore apples, and the threshold discrimination model based on two-band ratio showed great potential for detection of watercore apples.

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