RESUMEN
OBJECTIVE: To investigate the effects of hyperbaric oxygen preconditioning (HBO-PC) on neuronal apoptosis, Ca2+ concentration, and Caspases expression after spinal cord injury (SCI) in rats. MATERIALS AND METHODS: A total of 36 rats were randomly divided into control group (CON group), hyperbaric oxygen preconditioning group (HBO-PC group) and spinal cord injury group (SCI group), with 12 rats in each group. Rats in group HBO-PC were given HBO-PC intervention before modeling. SCI model was established by modified Allen method in group HBO-PC and group SCI. Basso-Beattie-Bresnahan (BBB) locomotor rating scale and motor evoked potential (MEP) examination were used to assess the neurological function. The expression of apoptosis gene caspase (3, 7, 8, 12) mRNA was detected by reverse transcription-polymerase chain reaction (RT-PCR). The concentration of Ca2+ in spinal cord tissue of each group was detected. RESULTS: CON group, HBO-PC group, and SCI group were gradually diminishing in BBB score and potential value and amplitude of MEP, respectively. The differences between groups were statistically significant (p<0.05). The expressions of Caspase-3 and 7, 8 and 12 mRNA in SCI group were significantly higher than those in CON group and HBO-PC group, respectively (p<0.05). There was no significant difference between CON group and HBO-PC group (p>0.05). The concentrations of Ca2+ in the CON group, HBO-PC group and SCI group were gradually increased; differences between groups were statistically significant (p<0.05). CONCLUSIONS: HBO-PC can reduce the loss of motor function of SCI rats, which may inhibit the activation of endoplasmic reticulum pathway of neural apoptosis, and reduce the calcium overload through inhibiting the expressions of pro-apoptotic proteins (Caspase-3/7/8/12), thus reducing the cell apoptosis and protecting neurons.
Asunto(s)
Apoptosis , Calcio/metabolismo , Caspasas/biosíntesis , Oxigenoterapia Hiperbárica , Neuronas/patología , Traumatismos de la Médula Espinal/metabolismo , Traumatismos de la Médula Espinal/patología , Animales , Potenciales Evocados Motores/fisiología , Femenino , Locomoción/fisiología , Masculino , Ratas , Traumatismos de la Médula Espinal/enzimologíaRESUMEN
The results of treating chronic lymphedema using microwave heating are described in 45 patients with postmastectomy lymphedema of the upper extremity. Forty-five patients showed a significant (p < 0.01) reduction in the amount of peripheral edema after two courses of treatment. The incidence and severity of secondary acute inflammation (erysipelas) were also sharply reduced (p < 0.01). The elasticity of the soft tissue was restored.
Asunto(s)
Brazo , Calor/uso terapéutico , Linfedema/terapia , Mastectomía , Microondas , Complicaciones Posoperatorias/terapia , Adulto , Anciano , Erisipela/etiología , Erisipela/terapia , Femenino , Humanos , Linfedema/etiología , Masculino , Persona de Mediana Edad , Complicaciones Posoperatorias/etiología , Índice de Severidad de la EnfermedadRESUMEN
We reported that malignant cells are inactivated by photo-excited TiO2 particles (Cai, R.-X., et al. (1992) Cancer Res. 52, 2346-2348). In the present study, the process of cell death of a human bladder cell line T24 with the irradiated TiO2 was investigated by monitoring the time course change in intracellular calcium concentration ([Ca2+]i) under UV irradiation using calcium fluorescence indicator, fura-2. In the presence of photo-excited TiO2 particles (100 micrograms/ml), [Ca2+]i showed a rapid two-step increase; while in the absence of TiO2, it exhibited only a slight and monotonous increase or maintained a constant level. The rapid elevation of [Ca2+]i in the presence of photo-excited TiO2 particles was caused by the influx of extracellular Ca2+ through the plasma membrane and cell death occurred only after the second rapid elevation of [Ca2+]i. These results suggested that the cell membrane permeability to Ca2+ was promoted prior to cell death.
Asunto(s)
Calcio/análisis , Titanio/farmacología , Permeabilidad de la Membrana Celular , Supervivencia Celular , Etidio , Fura-2 , Humanos , Factores de Tiempo , Titanio/efectos de la radiación , Células Tumorales Cultivadas/efectos de los fármacos , Células Tumorales Cultivadas/ultraestructura , Rayos UltravioletaRESUMEN
A patient with carcinosarcoma of the urethra occurring 15 years after a radical cystoprostatectomy for transitional cell carcinoma is presented. The tumor arose from the penile urethra and invaded the glans penis and penile corpus cavernosum. The tumor was composed of mixed carcinoma (squamous cell carcinoma, adenocarcinoma and transitional cell carcinoma) and rhabdomyosarcoma. Biopsy of the right inguinal lymph node showed simultaneous metastasis of sarcomatous and transitional cell carcinoma elements.