RESUMEN
General anesthetics produce anesthesia by depressing central nervous system activity. Activation of inhibitory GABA(A) receptors plays a central role in the action of many clinically relevant general anesthetics. Even so, there is growing evidence that anesthetics can act at a presynaptic locus to inhibit neurotransmitter release. Our own data identified the neurotransmitter release machinery as a target for anesthetic action. In the present study, we sought to examine the site of anesthetic action more closely. Exocytosis was stimulated by directly elevating the intracellular Ca(2+) concentration at neurotransmitter release sites, thereby bypassing anesthetic effects on channels and receptors, allowing anesthetic effects on the neurotransmitter release machinery to be examined in isolation. Three different PC12 cell lines, which had the expression of different release machinery proteins stably suppressed by RNA interference, were used in these studies. Interestingly, there was still significant neurotransmitter release when these knockdown PC12 cells were stimulated. We have previously shown that etomidate, isoflurane, and propofol all inhibited the neurotransmitter release machinery in wild-type PC12 cells. In the present study, we show that knocking down synaptotagmin I completely prevented etomidate from inhibiting neurotransmitter release. Synaptotagmin I knockdown also diminished the inhibition produced by propofol and isoflurane, but the magnitude of the effect was not as large. Knockdown of SNAP-25 and SNAP-23 expression also changed the ability of these three anesthetics to inhibit neurotransmitter release. Our results suggest that general anesthetics inhibit the neurotransmitter release machinery by interacting with multiple SNARE and SNARE-associated proteins.
Asunto(s)
Anestésicos Generales/farmacología , Catecolaminas/metabolismo , Exocitosis/efectos de los fármacos , Proteína 25 Asociada a Sinaptosomas/genética , Sinaptotagmina I/genética , Animales , Etomidato/farmacología , Expresión Génica , Isoflurano/farmacología , Células PC12 , Propofol/farmacología , ARN Interferente Pequeño , Ratas , Vesículas Sinápticas/efectos de los fármacos , Vesículas Sinápticas/metabolismo , Proteína 25 Asociada a Sinaptosomas/metabolismo , Sinaptotagmina I/metabolismoRESUMEN
In sympathetic neurons, it is well-established that the neurotransmitters, norepinephrine (NE), neuropeptide Y (NPY), and ATP are differentially coreleased from the same neurons. In this study, we determined whether synaptotagmin (syt) I, the primary Ca(2+) sensor for regulated release, could function as the protein that differentially regulates release of these neurotransmitters. Plasmid-based RNA interference was used to specifically and stably silence expression of syt I in a model secretory cell line. Whereas stimulated release of NPY and purines was abolished, stimulated catecholamine (CA) release was only reduced by approximately 50%. Although expression levels of tyrosine hydroxylase, the rate-limiting enzyme in the dopamine synthesis pathway, was unaffected, expression of the vesicular monoamine transporter 1 was reduced by 50%. To evaluate whether NPY and CAs are found within the same vesicles and whether syt I is found localized to each of these NPY- and CA-containing vesicles, we used immunocytochemistry to determine that syt I colocalized with large dense core vesicles, with NPY, and with CAs. Furthermore, both CAs and NPY colocalized with one another and with large dense core vesicles. Electron micrographs show that large dense core vesicles are synthesized and available for release in cells that lack syt I. These results are consistent with syt I regulating differential release of transmitters.
Asunto(s)
Dopamina/metabolismo , Neuropéptido Y/metabolismo , Norepinefrina/metabolismo , Vesículas Sinápticas/metabolismo , Sinaptotagmina I/metabolismo , Adenosina Trifosfato/metabolismo , Animales , Expresión Génica , Inmunohistoquímica , Microscopía Electrónica de Transmisión , Células PC12 , Interferencia de ARN , Ratas , Vesículas Sinápticas/ultraestructura , Sinaptotagmina I/genética , Tirosina 3-Monooxigenasa/metabolismoRESUMEN
RNA interference (RNAi) has quickly become a very powerful technique for specifically suppressing or knocking down the expression of any desired gene. Many fields of research, including neuroscience, have benefitted from RNAi methods. It has been well documented that different small interfering RNAs (siRNAs) and small hairpin RNAs (shRNAs) vary greatly in terms of their effectiveness, and much attention has been focused on guidelines and algorithms for the selection of effective siRNAs. However, it has not been widely appreciated that a single shRNA-expressing plasmid can also produce widely varying levels of knockdown in different stably transfected cell lines derived from the same transfection. Here we report that knockdown of three distinct target proteins varies from minimal to almost complete in independent, stably transfected PC12 cell lines. This variability in knockdown among cell lines emphasizes the importance of characterizing a number of cell lines when attempting to establish stable knockdown cell lines, but also offers the possibility of studying the effects of graded levels of protein expression.
Asunto(s)
Silenciador del Gen/efectos de los fármacos , Silenciador del Gen/fisiología , Interferencia de ARN/fisiología , ARN Interferente Pequeño/farmacología , Animales , Regulación Neoplásica de la Expresión Génica/efectos de los fármacos , Proteínas Fluorescentes Verdes/metabolismo , Células PC12/fisiología , Procesamiento Postranscripcional del ARN/efectos de los fármacos , Procesamiento Postranscripcional del ARN/fisiología , Ratas , Transfección/métodosRESUMEN
Adaptor protein 3 (AP-3) is a vesicle-coat protein that forms a heterotetrameric complex. Two types of AP-3 subunits are found in mammalian cells. Ubiquitous AP-3 subunits are expressed in all tissues of the body, including the brain. In addition, there are neuronal AP-3 subunits that are thought to serve neuron-specific functions such as neurotransmitter release. In this study, we show that overexpression of neuronal AP-3 in mouse chromaffin cells results in a striking decrease in the neurotransmitter content of individual vesicles (quantal size), whereas deletion of all AP-3 produces a dramatic increase in quantal size; these changes were correlated with alterations in dense-core vesicle size. AP-3 appears to localize in the trans-Golgi network and possibly immature secretory vesicles, where it may be involved in the formation of neurosecretory vesicles.
Asunto(s)
Complejo 3 de Proteína Adaptadora/fisiología , Células Cromafines/ultraestructura , Neurotransmisores/metabolismo , Vesículas Secretoras/ultraestructura , Complejo 3 de Proteína Adaptadora/genética , Animales , Células Cultivadas , Células Cromafines/metabolismo , Ratones , Ratones Mutantes , Mutación , Neuronas/metabolismo , Neuronas/ultraestructura , Vesículas Secretoras/metabolismo , Eliminación de Secuencia , Activación Transcripcional , Red trans-Golgi/metabolismo , Red trans-Golgi/ultraestructuraRESUMEN
Synaptotagmin (syt) I is a Ca2+-binding protein that is well accepted as a major sensor for Ca2+-regulated release of transmitter. However, controversy remains as to whether syt I is the only protein that can function in this role and whether the remaining syt family members also function as Ca2+ sensors. In this study, we generated a PC12 cell line that continuously expresses a short hairpin RNA (shRNA) to silence expression of syt I by RNA interference. Immunoblot and immunocytochemistry experiments demonstrate that expression of syt I was specifically silenced in cells that stably integrate the shRNA-syt I compared with control cells stably transfected with the empty shRNA vector. The other predominantly expressed syt isoform, syt IX, was not affected, nor was the expression of the SNARE (soluble N-ethylmaleimide-sensitive factor attachment protein receptor) proteins when syt I levels were knocked down. Resting Ca2+ and stimulated Ca2+ influx imaged with fura-2 were not altered in syt I knockdown cells. However, evoked release of catecholamine detected by carbon fiber amperometry and HPLC was significantly reduced, although not abolished. Human syt I rescued the release events in the syt I knockdown cells. The reduction of stimulated catecholamine release in the syt I knockdown cells strongly suggests that although syt I is clearly involved in catecholamine release, it is not the only protein to regulate stimulated release in PC12 cells, and another protein likely has a role as a Ca2+ sensor for regulated release of transmitter.
Asunto(s)
Calcio/metabolismo , Catecolaminas/metabolismo , Fusión de Membrana/fisiología , Vesículas Sinápticas/metabolismo , Sinaptotagmina I/metabolismo , Animales , Silenciador del Gen , Células PC12 , Ratas , Sinaptotagmina I/genéticaRESUMEN
BACKGROUND: SNAP-25 is a synaptic protein known to be involved in exocytosis of synaptic vesicles in neurons and of large dense-core vesicles in neuroendocrine cells. Its role in exocytosis has been studied in SNAP-25 knockout mice, in lysed synaptosomes lacking functional SNAP-25 and in cells after treatment with botulinum toxins A or E that specifically cleave SNAP-25. These studies have shown that SNAP-25 appears to be required for most but not all evoked secretion. In order to further study the role of SNAP-25 in catecholamine secretion from PC12 cells we have used the recently developed technique of RNA interference to generate PC12 cell lines with virtually undetectable levels of SNAP-25. RNA interference is the sequence-specific silencing or knockdown of gene expression triggered by the introduction of double-stranded RNA into a cell. RNA interference can be elicited in mammalian cells in a number of ways, one of which is by the expression of small hairpin RNAs from a transfected plasmid. Selection of stably transfected cell lines expressing a small hairpin RNA allows one-time characterization of the degree and specificity of gene silencing and affords a continuing source of well-characterized knockdown cells for experimentation. RESULTS: A PC12 cell line stably transfected with a plasmid expressing an shRNA targeting SNAP-25 has been established. This SNAP-25 knockdown cell line has barely detectable levels of SNAP-25, but normal levels of other synaptic proteins. Catecholamine secretion elicited by depolarization of the SNAP-25 knockdown cells was reduced to 37% of control. CONCLUSION: Knockdown of SNAP-25 in PC12 cells reduces but does not eliminate evoked secretion of catecholamines. Transient expression of human SNAP-25 in the knockdown cells rescues the deficit in catecholamine secretion.
Asunto(s)
Silenciador del Gen , Proteína 25 Asociada a Sinaptosomas/genética , Transfección/métodos , Animales , Catecolaminas/metabolismo , Estimulación Eléctrica , Exocitosis/fisiología , Expresión Génica/genética , Células PC12 , Plásmidos , RatasRESUMEN
Presynaptic N-type Ca2+ channels (CaV2.2, alpha1B) are thought to bind to SNARE (SNAP-25 receptor) complex proteins through a synaptic protein interaction (synprint) site on the intracellular loop between domains II and III of the alpha1B subunit. Whether binding of syntaxin to the N-type Ca2+ channels is required for coupling Ca2+ ion influx to rapid exocytosis has been the subject of considerable investigation. In this study, we deleted the synprint site from a recombinant alpha1B Ca2+ channel subunit and transiently transfected either the wild-type alpha1B or the synprint deletion mutant into mouse pheochromocytoma (MPC) cell line 9/3L, a cell line that has the machinery required for rapid stimulated exocytosis but lacks endogenous voltage-dependent Ca2+ channels. Secretion was elicited by activation of exogenously transfected Ca2+ channel subunits. The current-voltage relationship was similar for the wild-type and mutant alpha1B-containing Ca2+ channels. Although total Ca2+ entry was slightly larger for the synprint deletion channel, compared with the wild-type channel, when Ca2+ entry was normalized to cell size and limited to cells with similar Ca2+ entry (approximately 150 x 10(6) Ca2+ ions/pF cell size), total secretion and the rate of secretion, determined by capacitance measurements, were significantly reduced in cells expressing the synprint deletion mutant channels, compared with wild-type channels. Furthermore, the amount of endocytosis was significantly reduced in cells with the alpha1B synprint deletion mutant, compared with the wild-type subunit. These results suggest that the synprint site is necessary for efficient coupling of Ca2+ influx through alpha1B-containing Ca2+ channels to exocytosis.
Asunto(s)
Canales de Calcio Tipo N/química , Canales de Calcio Tipo N/metabolismo , Animales , Sitios de Unión/genética , Canales de Calcio Tipo N/genética , Señalización del Calcio , Línea Celular Tumoral , Exocitosis , Ratones , Datos de Secuencia Molecular , Neurotransmisores/metabolismo , Feocromocitoma/genética , Feocromocitoma/metabolismo , Proteínas Recombinantes/química , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Proteínas SNARE , Eliminación de Secuencia , Sinapsis/metabolismo , Transfección , Proteínas de Transporte Vesicular/metabolismoRESUMEN
N- and P/Q-type Ca2+ channels are abundant in nerve terminals where they interact with proteins of the release apparatus, including syntaxin 1A and SNAP-25. In previous studies on N- or P/Q-type Ca2+ channels, syntaxin 1A co-expression reduced current amplitudes, increased voltage-dependent inactivation and/or enhanced G-protein inhibition. However, these studies were conducted in Ca2+ channels that exhibited significant voltage-dependent inactivation. We previously reported that N-type current in bovine chromaffin cells exhibits very little voltage-dependent inactivation and we identified the Ca2+ channel subunits involved. This study was undertaken to determine the effect of syntaxin 1A on this weakly inactivating Ca2+ channel. Co-expression of syntaxin 1A with the weakly inactivating bovine N-type Ca2+ channels in Xenopus oocytes did not appear to alter inactivation but dramatically reduced current amplitudes, without changing cell surface expression. To further understand the mechanisms of syntaxin 1A regulation of this weakly inactivating channel, we examined mutants of the alpha1B subunit, beta2a subunit and syntaxin 1A. We determined that the synprint site of alpha1B and the C-terminal third of syntaxin 1A were necessary for the reduced current amplitude. In addition we show that enhanced G-protein-dependent modulation of the Ca2+ current by syntaxin 1A cannot explain the large suppression of Ca2+ current observed. Of most significance, syntaxin 1A increased voltage-dependent inactivation in channels containing mutant beta2a subunits that cannot be palmitoylated. Our data suggest that changes in inactivation can not explain the reduction in current amplitude produced by co-expressing syntaxin and a weakly inactivating Ca2+ channel.
Asunto(s)
Antígenos de Superficie/farmacología , Canales de Calcio Tipo N/efectos de los fármacos , Canales de Calcio Tipo N/metabolismo , Proteínas del Tejido Nervioso/farmacología , Animales , Antígenos de Superficie/química , Antígenos de Superficie/genética , Bloqueadores de los Canales de Calcio/metabolismo , Canales de Calcio Tipo N/genética , Canales de Calcio Tipo N/fisiología , Bovinos , Conductividad Eléctrica , Femenino , Proteínas de Unión al GTP/fisiología , Mutación/fisiología , Proteínas del Tejido Nervioso/química , Proteínas del Tejido Nervioso/genética , Oocitos/efectos de los fármacos , Oocitos/metabolismo , Isoformas de Proteínas/genética , Estructura Terciaria de Proteína/fisiología , Ratas , Sintaxina 1 , Xenopus , omega-Conotoxina GVIA/metabolismoRESUMEN
We have characterized a recently established mouse pheochromocytoma cell line (MPC 9/3L) as a useful model for studying neurotransmitter release and neuroendocrine secretion. MPC 9/3L cells express many of the proteins involved in Ca2+-dependent neurotransmitter release but do not express functional endogenous Ca2+-influx pathways. When transfected with recombinant N-type Ca2+ channel subunits alpha1B,beta2a,alpha2delta (Cav2.2), the cells expressed robust Ca2+ currents that were blocked by omega-conotoxin GVIA. Activation of N-type Ca2+ currents caused rapid increases in membrane capacitance of the MPC 9/3L cells, indicating that the Ca2+ influx was linked to exocytosis of vesicles similar to that reported in chromaffin or PC12 cells. Synaptic protein interaction (synprint) sites, like those found on N-type Ca2+ channels, are thought to link voltage-dependent Ca2+ channels to SNARE proteins involved in synaptic transmission. Interestingly, MPC 9/3L cells transfected with either LC-type (alpha1C, beta2a, alpha2delta, Cav1.2) or T-type (alpha1G, beta2a, alpha2delta, Cav3.1) Ca2+ channel subunits, which do not express synprint sites, expressed appropriate Ca2+ currents that supported rapid exocytosis. Thus MPC 9/3L cells provide a unique model for the study of exocytosis in cells expressing specific Ca2+ channels of defined subunit composition without complicating contributions from endogenous channels. This model may help to distinguish the roles that different Ca2+ channels play in Ca2+-dependent secretion.