RESUMEN
This study explores acid and alkaline pretreatments in order to enhance soybean straw biodegradability. The effects of sulfuric acid and sodium hydroxide for different pretreatment times at 30°C and 121°C on biomass dissolution and the subsequent enzymatic hydrolysis were investigated. The highest total conversion to reducing sugars of 93.9% was attained when soybean straw was pretreated with acid (4% H2SO4, 121°C, 1 h) and subsequently subjected to the enzymatic process. However, conversion of 86.5%, were reached only with the hydrolysis of the pretreated residue using mild conditions, (0.5% NaOH, 30°C, 48 h), involving the reduction cost of the process. In addition to this, this result was dramatically decreased when pectinase was removed from the enzyme cocktail. It has been also demonstrated that the reduction of the enzyme loading to less than half allowed obtaining about 96% of the reducing sugars attained with the highest enzyme dose.
Asunto(s)
Biotecnología/métodos , Glycine max/química , Hidróxido de Sodio/química , Ácidos Sulfúricos/química , Biodegradación Ambiental , Biomasa , Hidrólisis , Tallos de la Planta/química , Poligalacturonasa/química , Poligalacturonasa/metabolismo , Polisacáridos/química , Polisacáridos/metabolismo , ResiduosRESUMEN
The current study explores alkaline and alkaline peroxide pretreatments in order to achieve a method to improve saccharification of agricultural residues for ethanol production. The effects of reagent concentration and pretreatment time at 30°C and atmospheric pressure on biomass dissolution after the pretreatment and enzymatic hydrolysis of the pretreated biomass were investigated. In fact, although all pretreatments tested improved enzymatic hydrolysis of native residues, the best results were not achieved for the highest biomass loss. The maximum conversions to reducing sugars in the hydrolysis stage of 77.5% and 92.6% were obtained for rice hulls and straw pretreated by alkaline peroxide (4%, 24h) and alkaline (1%, 48 h) methods, respectively. For both pretreated residues, the reduction to more than half the recommended enzyme loading allowed obtaining more than 94% the reducing sugars attained with the recommended dose.