RESUMEN
The caspase-3-cleaved presence was evaluated in this study in the heart of irradiated rats, during the decline of ventricular function. Female Wistar rats were irradiated with a single dose of radiation (15 Gy) delivered directly to the heart and the molecular, histological and physiological evaluations were performed at thirteen months post-irradiation. The expressions of procollagen type I, TGF-ß1 and caspase-3-cleaved were analyzed using Western blotting. Cardiac structural and functional alterations were investigated by echocardiography and electron microscopy. In the irradiated group, the levels of procollagen type I, TGF-ß1 and caspase-3-cleaved are increased. Significant histological changes (degeneration of heart tissue and collagen deposition) and functional (reduced ejection fraction) were observed. Data suggest that the cardiac function decline after exposure to ionizing radiation is related, in part, to increased collagen and increased caspase-3-cleaved.
Asunto(s)
Caspasa 3/metabolismo , Colágeno Tipo I/metabolismo , Corazón/efectos de la radiación , Miocardio/enzimología , Factor de Crecimiento Transformador beta1/metabolismo , Animales , Western Blotting , Relación Dosis-Respuesta en la Radiación , Ecocardiografía , Activación Enzimática/efectos de la radiación , Femenino , Microscopía Electrónica de Transmisión , Miocardio/ultraestructura , Ratas , Ratas WistarRESUMEN
Nucleotide excision repair (NER) is the most flexible of all known DNA-repair mechanisms, and XPG is a 3'-endonuclease that participates in NER. Mutations in this gene (ERCC5) may result in the human syndrome xeroderma pigmentosum (XP) and, in some cases, in the complex phenotype of Cockayne syndrome (CS). Two Brazilian XP siblings, who were mildly affected, were investigated and classified into the XP-G group. The cells from these patients were highly ultraviolet (UV) sensitive but not sensitive to photosensitized methylene blue, an agent that causes oxidative stress. This phenotype is in contrast to XP-G/CS cells, which are highly sensitive to this oxidative agent. Sequencing revealed a compound heterozygous genotype with two novel missense mutations: c.83C>A (p.Ala28Asp) and c.2904G>C (p.Trp968Cys). The first mutation maps to the catalytic site of the XPG protein, whereas the second may compromise binding to DNA. Functional assays indicated that the mutated alleles were unable to perform the complete repair of UV-irradiated plasmids; however, full correction was observed for oxidatively damaged plasmids. Therefore, the XP phenotype of these patients is caused by novel missense mutations that specifically affect DNA repair for UV- but not oxidative-stress-induced DNA damage, and implications for XP versus XP/CS phenotype are discussed.
Asunto(s)
Supervivencia Celular/efectos de la radiación , Reparación del ADN/efectos de la radiación , Proteínas de Unión al ADN/genética , Endonucleasas/genética , Proteínas Nucleares/genética , Factores de Transcripción/genética , Adolescente , Alelos , Secuencia de Aminoácidos , Brasil , Línea Celular , Clonación Molecular , Síndrome de Cockayne/genética , Daño del ADN/efectos de la radiación , Femenino , Fibroblastos/citología , Fibroblastos/efectos de la radiación , Heterocigoto , Humanos , Masculino , Datos de Secuencia Molecular , Mutación Missense , Estrés Oxidativo/efectos de la radiación , Fenotipo , Conformación Proteica , Alineación de Secuencia , Análisis de Secuencia de ADN , Rayos Ultravioleta , Xerodermia Pigmentosa/genética , Adulto JovenRESUMEN
Lanthanum (La) is a rare-earth metal with applications in agriculture, industry, and medicine. Since lanthanides show a broad spectrum of applications there is an increased risk of contamination for humans. We examined the effects of lanthanum in Jurkat cells and human peripheral lymphocytes (HPL), and we found that it was cytotoxic and genotoxic on both cell lines. Additionally, HPL were more sensitive to La treatment than Jurkat cells and necrosis was the pathway by which La induced cytotoxicity. Vitamin E was able to diminish the DNA strand breaks induced suggesting that oxidative stress may be involved in the genotoxic process.
Asunto(s)
Carcinógenos/toxicidad , Contaminantes Ambientales/toxicidad , Lantano/toxicidad , Linfocitos/efectos de los fármacos , Mutágenos/toxicidad , Supervivencia Celular/efectos de los fármacos , Daño del ADN/efectos de los fármacos , Relación Dosis-Respuesta a Droga , Antagonismo de Drogas , Combinación de Medicamentos , Contaminantes Ambientales/clasificación , Humanos , Células Jurkat , Lantano/clasificación , Linfocitos/patología , Necrosis/inducido químicamente , Vitamina E/farmacologíaRESUMEN
Oxyygen free radicals are highly reactive species that damage several cellular macromolecules and organelles, including membrane lipid peroxidation and produce DNA lesions. We have discussed here; i) The mechanism of radiation-induced cellular damage in bacteria through the intermediation of active oxygen species; ii) the cellular inactivation and the role of bacterial SOS and OxyR systems in the repair of lesions induced by H2O2 under low iron condition; iii) the lethal interaction between H2O2 and o-phenanthroline in E. coli; iv) the biological response induced by near-UV radiation mediated by active oxygen species and finally v) the mutagenic potential of popular plant extracts like guaraná (Paullinia cupana), mate (Ilex paraguariensis) and saiao (Kalanchoe brasiliensis), whose effects are eventually mediated by active oxygen species.