RESUMEN
Somatolactin (SL) and SL receptor (SLR) belong to the growth hormone and cytokine type I receptor superfamilies, respectively. However, further research is required to define the duplications and functions of SL and its receptors in basal vertebrates including environmental background color adaptation in fish. In the present study, we cloned and sequenced SL and its putative receptor (SLR), classified and compared the sequences phylogenetically, and determined SL and SLR mRNA expression levels during long-term background color exposure in Cichlasoma dimerus, a freshwater South American cichlid. Our results show that C. dimerus SL and SLR share high sequence similarity with homologous from other perciform fish. Phylogenetic analysis indicates that C. dimerus SL belongs to the SLα clade sub-group. C. dimerus SLR is clearly a member of the GHR1 receptor subgroup, which includes the experimentally validated SLR from salmonids. Higher transcript levels of SLα in the pituitary and SLR in the epidermis and dermis cells of fish scales were observed in fish following long-term black background color exposure compared to those exposed to a white background. A higher number of melanophores was also observed in fish exposed for 10days to a black background compared to those exposed to a white background. These changes were concomitant to differences in SL or SLR transcript levels found in fish exposed to these two different background colors. Our results suggest, for the first time, that SLR is expressed in fish scales, and that there is an increase in SL in the pituitary and the putative SLR in likely target cells, i.e., melanophores, in long-term black background exposure in C. dimerus.
Asunto(s)
Aclimatación/genética , Cíclidos/genética , Proteínas de Peces/genética , Glicoproteínas/genética , Hormonas Hipofisarias/genética , Receptores de la Hormona Hipofisaria/genética , Receptores de Somatotropina/genética , Pigmentación de la Piel/genética , Secuencia de Aminoácidos , Animales , Secuencia de Bases , Cíclidos/fisiología , Clonación Molecular , Color , Ambiente , Proteínas de Peces/fisiología , Glicoproteínas/fisiología , Melanóforos/fisiología , Datos de Secuencia Molecular , Filogenia , Hormonas Hipofisarias/fisiología , ARN Mensajero/metabolismo , Receptores de la Hormona Hipofisaria/fisiología , Receptores de Somatotropina/fisiologíaRESUMEN
Many characteristics of the South American teleost fish Cichlasoma dimerus (body size, easy breeding, undemanding maintenance) make it amenable to laboratory studies. In the last years, many of the fundamental aspects of its reproductive and developmental biology have been addressed in our laboratory. Rather recently, the immunohistochemical localization of pituitary hormones involved in reproduction and in background color adaptation has been described in both adult and developing individuals, and the role of FSH in ovarian differentiation has been established. These findings have been correlated with mapping of some of their brain-derived controlling hormones. The latter include brain-derived gonadotropins which were shown to be active in vitro in the control of pituitary hormone secretions. The emerging picture shows C. dimerus as an interesting species in which many of their basic features have already been investigated and which conform a solid platform for comparative studies correlating neurohormones, pituitary hormones and behavior, from the molecular to the organismic level.
Asunto(s)
Cíclidos/embriología , Cíclidos/fisiología , Gonadotropinas/metabolismo , Ovario/embriología , Hormonas Hipofisarias/metabolismo , Testículo/embriología , Animales , Encéfalo/metabolismo , Femenino , Masculino , Ovario/citología , Hipófisis/anatomía & histología , Hipófisis/metabolismo , Reproducción , Testículo/citologíaRESUMEN
Many characteristics of the South American teleost fish Cichlasoma dimerus (body size, easy breeding, undemanding maintenance) make it amenable to laboratory studies. In the last years, many of the fundamental aspects of its reproductive and developmental biology have been addressed in our laboratory. Rather recently, the immunohistochemical localization of pituitary hormones involved in reproduction and in background color adaptation has been described in both adult and developing individuals, and the role of FSH in ovarian differentiation has been established. These findings have been correlated with mapping of some of their brain-derived controlling hormones. The latter include brain-derived gonadotropins which were shown to be active in vitro in the control of pituitary hormone secretions. The emerging picture shows C. dimerus as an interesting species in which many of their basic features have already been investigated and which conform a solid platform for comparative studies correlating neurohormones, pituitary hormones and behavior, from the molecular to the organismic level.(AU)
Asunto(s)
Masculino , Animales , Femenino , Encéfalo/metabolismo , Cíclidos/embriología , Cíclidos/fisiología , Gonadotropinas/metabolismo , Ovario/embriología , Hipófisis/metabolismo , Testículo/citología , Testículo/embriología , Hormonas Hipofisarias/metabolismo , ReproducciónRESUMEN
Many characteristics of the South American teleost fish Cichlasoma dimerus (body size, easy breeding, undemanding maintenance) make it amenable to laboratory studies. In the last years, many of the fundamental aspects of its reproductive and developmental biology have been addressed in our laboratory. Rather recently, the immunohistochemical localization of pituitary hormones involved in reproduction and in background color adaptation has been described in both adult and developing individuals, and the role of FSH in ovarian differentiation has been established. These findings have been correlated with mapping of some of their brain-derived controlling hormones. The latter include brain-derived gonadotropins which were shown to be active in vitro in the control of pituitary hormone secretions. The emerging picture shows C. dimerus as an interesting species in which many of their basic features have already been investigated and which conform a solid platform for comparative studies correlating neurohormones, pituitary hormones and behavior, from the molecular to the organismic level.