RESUMEN
PURPOSE: The aim of the present study was to assess the effect of tumor advancement and surgery treatment on P-selectin expression (CD 62P), level of sP-selectin and platelet count. MATERIAL AND METHODS: The study involved 27 colorectal cancer patients (CRC). They were divided into two groups: group B1--18 patients (T2-3N0M0) and group B2--9 patients (T2-4N+M0). In CRC patients the blood was collected three times: 1) before surgery (A0), 2) 3 days after surgery and 3) 12 days after surgery. RESULTS: The results obtained showed that CD 62P expression in CRC patients was twice higher (5.36%) than in control (2.58%) (p < 0.001). The level of sP-selectin concentration in both groups (B1--74.22 ng/mL and B2--70.33 ng/mL) was significantly higher than in control (46.01 ng/mL) (p < 0.001). There was no significant differences in CD 62P expression, plasma sP-selectin concentration and in PLT count between group B1 and B2. Three days after surgery in both groups of patients we observed decreased CD 62P expression and sP-selectin level compared to the results before surgery (p < 0.05). Twelve days after surgery we found an increase in the CD 62P-positive platelets and sP-selectin in group B1 and B2. We found positive correlation between plasma sP-selectin concentration and PLT count in CRC. CONCLUSIONS: In the current study on colorectal cancer we observed platelet hyperactivation, irrespective of tumor clinical advancement. Surgical procedure, in the early period following radical tumor resection, does not totally eliminate platelet activation in vivo.
Asunto(s)
Plaquetas/metabolismo , Neoplasias Colorrectales/sangre , Neoplasias Colorrectales/cirugía , Selectina-P/sangre , Anciano , Anciano de 80 o más Años , Femenino , Citometría de Flujo , Humanos , Masculino , Persona de Mediana Edad , Recuento de PlaquetasRESUMEN
PURPOSE: Smoking is a significant risk factor of cardiac ischaemia. Changes in platelet count, morphology and platelet activation enhance the risk. MATERIAL AND METHODS: The objective of the study was to assess platelet parameters in smoking healthy subjects with reference to sex. In the group of women, 27% were smokers, in the group of men--49%. All the subjects were tested for platelet count (PLT), mean platelet volume (MPV), percentage of large platelets (L(PLT)), concentrations of beta-thromboglobulin, sP-selectin (soluble) and thrombopoietin, percentage of reticulated platelets (RP) and absolute count of reticulated platelet. RESULTS: Lower platelet count (237.00 +/- 39.52 vs 258.34 +/- 40.81 x 10(9)/l, p = 0.0002), higher percentage of reticulated platelets (1.39 +/- 0 .66 vs 1.04 +/- 0.35%, p = 0.04) and higher concentration of sP-selectin (52.66 +/- 18.54 vs 43.94 +/- 17.14 ng/ml, p = 0.03) were observed only in the group of smoking women, compared to non-smokers. In neither of the sexes smoking had an effect on the following parameters: mean platelet volume, percentage of large platelets, concentration of thrombopoietin, absolute count of reticulated platelet and concentration of beta1 -thromboglobulin. CONCLUSIONS: The results allow the hypothesis that women are more sensitive to smoking than men. Platelets in male smokers are less sensitive to smoking--the study showed no significant changes in the parameters.
Asunto(s)
Activación Plaquetaria/fisiología , Fumar/fisiopatología , Trombopoyesis/fisiología , Adulto , Femenino , Humanos , Masculino , Selectina-P/sangre , Recuento de Plaquetas/métodos , Factores Sexuales , Trombopoyetina/sangre , beta-Tromboglobulina/metabolismoRESUMEN
Reticulocyte count by manual method has been the assay traditionally used to evaluate the status of erythropoiesis in hematological disorders with disturbances in erythropoietic activity. However, due to its variability, it is rather a semiquantitative method. Automated reticulocyte counting based on flow cytometry has provided more objective and exact measure of reticulocytes. Besides traditional parameters, such as percentage and absolute number of reticulocytes, automatic reticulocyte counters can detect differences in the amounts of cellular RNA present in immature erythrocytes that reflect their maturational stages and evaluate indices of reticulocyte maturation such as RMI, HFR, IRF. These parameters can be used as the earliest signs of marrow engraftment after autologous or allogeneic bone marrow transplantation. Currently there is no strict agreement between various automated methods of reticulocyte evaluation. Additionally, lack of standardization and quality control materials for this assay compels determination of own, interlaboratory ranges of reference values for new proposed parameters. A group of 102 children aged from 3 months to 18 years with normal hematological parameters was examined. Samples of blood stained supravitally with thiazole orange were analyzed in a flow cytometer. Results for percentage, absolute number and immature reticulocyte fraction expressed as a mean +/- 2SD were: 2.00 +/- 1.56%, 88.8 +/- 68.94 x 10(3)/microliter, and 0.22 +/- 0.16, respectively. A poor correlation was found between IRF and other parameters, suggesting its independent role as a marker of erythropoietic activity. Automated reticulocyte counting will probably improve the diagnosis and monitoring of many hematological diseases.