RESUMEN

Polymerase chain reaction (PCR) was developed for the detection of simian T-lymphotropic virus type 1 (STLV-1) infection of P. hamadryas and direct sequencing using oligo-nucleotide primer pairs specific for the tax and env regions of the related human T-lymphotropic virus type 1 (HTLV-1). Excellent specificity was shown in the detection of STLV-1 provirus in infected baboons by PCR using HTLV-1-derived primers. The nucleotide sequences of env 467bp and tax 159bp of the proviral genome (env position 5700-6137, tax position 7373-7498 HTLV-1, according to Seiki et al., 1983) derived from STLV-1-infected P. hamadryas were analysed using PCR and direct sequencing techniques. Two STLV-1 isolates from different sources (Sukhumi main-SuTLV-1 and forest stocks-STLV-1F) were compared. Two variants of STLV-1 among P. hamadryas with different level of homology to HTLV-1 were wound (83.8% and 95.2%, respectively). A possible role of nucleotide changes in env and tax sequenced fragments and oncogenicity of STLV-1 variants is discussed.

Asunto(s)

Variación Genética/genética , Papio/microbiología , Virus Linfotrópico T Tipo 1 de los Simios/genética , Animales , Secuencia de Bases , ADN Viral/genética , ADN Viral/aislamiento & purificación , Infecciones por Deltaretrovirus/microbiología , Infecciones por Deltaretrovirus/veterinaria , Georgia (República) , Ganglios Linfáticos/microbiología , Linfocitos/microbiología , Datos de Secuencia Molecular , Reacción en Cadena de la Polimerasa , Provirus/genética , Análisis de Secuencia de ADN