RESUMEN
We evaluated the utility of the commercial Allplex genital ulcer real-time PCR multiplex assay for detecting Treponema pallidum, herpes simplex virus 1 (HSV-1) and 2 (HSV-2), and Chlamydia trachomatis serovar L (lymphogranuloma venereum [LGV]) DNA in mucosal and genital ulcers in the context of suspected syphilis. In total, 374 documented genital and mucosal ulcers from patients with and without syphilis presenting at several sexually transmitted infection (STI) centers in France from October 2010 to December 2016 were analyzed at the National Reference Center (CNR) for Bacterial STIs at Cochin Hospital in Paris. T. pallidum subsp. pallidum detection results were compared with the final diagnosis based on a combination of clinical examination, serological results, and in-house nested PCR (nPCR). Detections of HSV and LGV were validated against reference methods. We found that 44.6% of the 374 samples tested were positive for T. pallidum subsp. pallidum, 21% for HSV, and 0.8% for LGV. No positive results were obtained for 30.7% of samples, and 4.8% presented coinfections. For T. pallidum subsp. pallidum detection, the overall sensitivity was 80% (95% confidence interval [CI], 76.1 to 84.1%), specificity was 98.8% (95% CI, 97.7 to 99.9%), positive predictive value was 98.8% (95% CI, 97.7 to 99.9%) and negative predictive value was 80.2% (95% CI, 76.2 to 84.2%), with a rate of concordance with the reference method of 92.5% (k = 0.85). This PCR multiplex assay is suitable for T. pallidum subsp. pallidum detection in routine use and facilitates the simultaneous rapid detection of a broad panel of pathogens relevant in a context of suspected syphilis lesions.
Asunto(s)
Sífilis , Treponema pallidum , Francia , Humanos , Reacción en Cadena de la Polimerasa Multiplex , Paris , Sífilis/diagnóstico , Treponema pallidum/genética , ÚlceraRESUMEN
Varicella-zoster virus (VZV) resistance to acyclovir (ACV) has only been reported in rare cases of immunocompromised patients. We report the first case of an immunocompetent patient with ACV-resistant VZV keratitis associated with a nucleotide deletion in the VZV thymidine kinase gene, leading to production of a truncated protein.
Asunto(s)
Aciclovir/uso terapéutico , Antivirales/uso terapéutico , Farmacorresistencia Viral , Herpesvirus Humano 3/efectos de los fármacos , Huésped Inmunocomprometido , Queratitis Herpética/tratamiento farmacológico , Queratitis Herpética/virología , Aciclovir/farmacología , Antivirales/farmacología , Herpesvirus Humano 3/genética , Humanos , Masculino , Persona de Mediana Edad , Proteínas Mutantes/genética , Eliminación de Secuencia , Timidina Quinasa/genéticaAsunto(s)
Anticuerpos Monoclonales/efectos adversos , Anticuerpos Antineoplásicos/efectos adversos , Antineoplásicos/efectos adversos , Subtipo H3N2 del Virus de la Influenza A , Gripe Humana/etiología , Aplasia Pura de Células Rojas/inducido químicamente , Aplasia Pura de Células Rojas/complicaciones , Alemtuzumab , Anticuerpos Monoclonales Humanizados , Enfermedad Crónica , Humanos , Masculino , Persona de Mediana EdadRESUMEN
We observed a prolonged shedding of virus 14 and 28 days after symptom onset in two patients with pandemic H1N1 influenza, who did not have immunodepression and were treated with neuraminidase inhibitor. This prolonged shedding was not associated with the emergence of resistance mutation H275Y in the viral neuraminidase gene.
Asunto(s)
Subtipo H1N1 del Virus de la Influenza A , Gripe Humana/diagnóstico , Gripe Humana/transmisión , Esparcimiento de Virus , Femenino , Francia , Humanos , Gripe Humana/virología , Masculino , Persona de Mediana Edad , Adulto JovenRESUMEN
The recent emergence of seasonal influenza A(H1N1) strains resistant to oseltamivir makes it necessary to monitoring carefully the susceptibility of human influenza viruses to neuraminidase inhibitors. We report the prevalence of the oseltamivir resistance among influenza A viruses circulating in south-western France over the past three years: seasonal influenza A(H1N1), seasonal influenza A(H3N2), and the influenza A(H1N1)v viruses associated with the ongoing 2009 pandemic. The main result of the study is the absence of oseltamivir resistance in the pandemic H1N1 strains studied so far (n=129).