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J Zoo Wildl Med ; 32(1): 17-24, 2001 Mar.
Artículo en Inglés | MEDLINE | ID: mdl-12790390

RESUMEN

Humoral immune responses of black-footed penguins (Spheniscus demersus) to DNA-mediated immunization with a beta-galactosidase reporter gene expression plasmid were evaluated. Six male and 6 female adult penguins received either test plasmid, pCMV-beta, containing the beta-galactosidase gene or control plasmid, pCI, lacking a gene for expression. Three birds from each group were used previously in a diluent control group and given one injection of sterile saline. All samples were screened for anti-beta-galactosidase antibodies by indirect enzyme-linked immunosorbent assay with anti-chicken immunoglobulin G as secondary antibody. Antibodies to beta-galactosidase were detected in the sera of pCMV-beta-inoculated penguins, with a peak response on day 21. Antibody titers of the test plasmid group versus both control groups on days 21, 28, and 42 differed significantly. These results demonstrate that black-footed penguins can be safely transfected with the gene encoding beta-galactosidase and will mount a humoral response against the in vivo-expressed protein. Knowledge from this initial study can be applied to the development of DNA-mediated vaccines against specific infectious diseases of penguins.


Asunto(s)
Formación de Anticuerpos , Enfermedades de las Aves/prevención & control , Aves/inmunología , Genes Reporteros , Vacunas de ADN/administración & dosificación , beta-Galactosidasa/inmunología , Animales , Animales de Zoológico , Ensayo de Inmunoadsorción Enzimática/veterinaria , Femenino , Inmunización/veterinaria , Inmunización Secundaria , Inmunoglobulina G/inmunología , Masculino , Plásmidos , Distribución Aleatoria , Vacunas de ADN/inmunología , beta-Galactosidasa/genética
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