1.
Appl Opt
; 32(6): 976-80, 1993 Feb 20.
Artículo
en Inglés
| MEDLINE
| ID: mdl-20802776
RESUMEN
Frequency-domain fluorometry relies on the measurement of the phase and amplitudes of the Fourier components of the time-dependent fluorescence signal. Experimental results that show that a conventional photomultiplier is subject to intensity-dependent phase shifts are presented. The measurements indicate that this is a problem well below the maximum linear current of the photomultiplier response. These results have important implications in frequency-domain fluorescence anisotropy experiments, in which the parallel and the perpendicular components of the emission intensity are inherently different from one another: a phase shift can be introduced by the photomultiplier.