RESUMEN
Reassortment contributes to the evolution of RNA viruses with segmented genomes, including Bluetongue virus (BTV). Recently, co-circulation of natural and vaccine BTV variants in Europe, and their ensuing reassortment, were proposed to promote appearance of novel European BTV strains, with potential implications for pathogenicity, spread and vaccination policies. Similarly, the geographical features of the Mediterranean basin, which spans over portions of three continents, may facilitate the appearance of clinically relevant reassortants via co-circulation of BTV strains of African, Asian and European origins. In August-October 2017, BTV serotype 6 (BTV-6) was identified in young animals exhibiting classical clinical signs of Bluetongue (BT) at Israeli sheep and cattle farms. Sequencing and pairwise analysis of this Israeli BTV-6 isolate revealed the closest sequence homology of its serotype-defining Segment 2 was with that of South African reference BTV-6 strain 5011 (93.88% identity). In contrast, the other viral segments showed highest homology (97.0%-99.47% identity) with BTV-3, -4 and -9 of Mediterranean and African origins. Specifically, four viral segments were nearly identical (99.13%-99.47%), with Tunisian and Italian BTV-3 strains (TUN2016 and SAD2018, correspondingly). Together, our data suggest that Mediterranean co-circulation and reassortment of BTV-3 and BTV-6 drove the emergence of a novel and virulent BTV-6 strain.
Asunto(s)
Virus de la Lengua Azul/genética , Lengua Azul/virología , Virus Reordenados/genética , Animales , Animales Domésticos/virología , Animales Salvajes/virología , Lengua Azul/epidemiología , Virus de la Lengua Azul/inmunología , Bovinos/virología , Enfermedades de los Bovinos/epidemiología , Enfermedades de los Bovinos/virología , Femenino , Israel/epidemiología , Italia/epidemiología , Masculino , Filogenia , ARN Viral/genética , Análisis de Secuencia de ADN , Homología de Secuencia , Serogrupo , Ovinos/virología , Enfermedades de las Ovejas/epidemiología , Enfermedades de las Ovejas/virología , Túnez/epidemiologíaRESUMEN
During September 2016-February 2017, we detected epizootic hemorrhagic disease virus (EHDV) in ruminants in Israel. BLAST and phylogenetic analyses of segment 2 in 6 EHDVs isolated from field samples indicated a close relationship to the EHDV serotype 1 strain in Nigeria. Affected cattle had mostly mild or asymptomatic disease.