RESUMEN
A modeling study was conducted to evaluate the acid-base chemistry of streams within Shenandoah National Park, Virginia and to project future responses to sulfur (S) and nitrogen (N) atmospheric emissions controls. Many of the major stream systems in the park have acid neutralizing capacity (ANC) less than 20 microeq/L, levels at which chronic and/or episodic adverse impacts on native brook trout are possible. Model hindcasts suggested that none of these streams had ANC less than 50 microeq/L in 1900. Model projections, based on atmospheric emissions controls representative of laws already enacted as of 2003, suggested that the ANC of those streams simulated to have experienced the largest historical decreases in ANC will increase in the future. The levels of S deposition that were simulated to cause streamwater ANC to increase or decrease to three specified critical levels (0, 20, and 50 microeq/L) ranged from less than zero (ANC level not attainable) to several hundred kg/ha/year, depending on the selected site and its inherent acid-sensitivity, selected ANC endpoint criterion, and evaluation year for which the critical load was calculated. Several of the modeled streams situated on siliciclastic geology exhibited critical loads <0 kg/ha/year to achieve ANC >50 microeq/L in the year 2040, probably due at least in part to base cation losses from watershed soil. The median modeled siliciclastic stream had a calculated critical load to achieve ANC >50 microeq/L in 2100 that was about 3 kg/ha/year, or 77% lower than deposition in 1990, representing the time of model calibration.
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Ácidos/análisis , Atmósfera/análisis , Ríos/química , Azufre/análisis , Contaminación Química del Agua/análisis , Lluvia Ácida/análisis , Monitoreo del Ambiente , VirginiaRESUMEN
Levcromakalim (LKM; a K(ATP) channel opener) reverses hypoxic pulmonary vasoconstriction in isolated pulmonary arteries and perfused lungs. This vasorelaxation is blocked by glibenclamide (GLB; a K(ATP) channel blocker). We evaluated the hemodynamic effect of LKM followed by GLB in a chronically instrumented neonatal porcine model of pulmonary hypertension, created by exposing piglets to hypoxia (n = 7) or heat-killed group B streptococci (GBS) (n = 6). Hypoxia increased pulmonary arterial pressure (PAP), which LKM decreased, and GLB subsequently increased in a dose-dependent manner. Systemic arterial pressure (SAP) did not change with hypoxia but was also decreased by LKM and increased by GLB. GBS also led to increased PAP, but LKM significantly reduced only SAP, which was then increased by GLB. We conclude LKM is capable of reversing hypoxic, but not GBS-induced, pulmonary hypertension but lacks specificity for the neonatal pulmonary vasculature.
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Animales Recién Nacidos , Cromakalim/uso terapéutico , Hemodinámica/efectos de los fármacos , Hipertensión Pulmonar/tratamiento farmacológico , Vasodilatadores/uso terapéutico , Animales , Presión Sanguínea/efectos de los fármacos , Modelos Animales de Enfermedad , Gliburida/farmacología , Hipertensión Pulmonar/etiología , Hipertensión Pulmonar/fisiopatología , Hipoxia , Activación del Canal Iónico/efectos de los fármacos , Canales de Potasio/efectos de los fármacos , Canales de Potasio/fisiología , Streptococcus agalactiae , Porcinos , Resistencia Vascular/efectos de los fármacosRESUMEN
RATIONALE: Cigarette smoke exposure in the perinatal period increases the risk of various prenatal and postnatal complications, including sudden infant death syndrome (SIDS) and persistent pulmonary hypertension of the newborn (PPHN). We investigated the cellular effects of cigarette smoke extract (CSE) in the developing vasculature. METHODS: Vascular smooth muscle cells (VSMC) were isolated from neonatal porcine carotid arteries, splenic arteries, and main and resistance pulmonary arteries. Effects of CSE on VSMC proliferation, viability, apoptosis, and media nitrates and nitrites were evaluated. The effects of known constituents of CSE (nicotine, benzopyrene, acrolein, acetaldehyde), aged CSE, CSE with added hemoglobin, devolatilized CSE, CSE with added dithiothreitol (DTT), and reduced glutathione (GSH) on cell proliferation and viability were assessed. RESULTS: CSE caused a dose- and time-dependent decrease in neonatal VSMC numbers isolated from all four sites, mainly as a result of increased cell necrosis and not apoptosis. Nitrates and nitrites were below the threshold of detection. Nicotine and benzopyrene did not affect cell counts, while acrolein and acetaldehyde decreased cell counts in a dose-dependent manner. Addition of hemoglobin, devolatilization, and the addition of DTT or GSH slightly decreased CSE inhibition. CONCLUSIONS: CSE causes necrosis of neonatal VSMC, and this toxicity is mediated mainly by volatile components such as acrolein and acetaldehyde, possibly in association with nitric oxide and carbon monoxide.
Asunto(s)
Músculo Liso Vascular/efectos de los fármacos , Nicotiana , Plantas Tóxicas , Humo/efectos adversos , Acetaldehído/toxicidad , Acroleína/toxicidad , Animales , Apoptosis/efectos de los fármacos , División Celular/efectos de los fármacos , Supervivencia Celular/efectos de los fármacos , Células Cultivadas , Medios de Cultivo , Músculo Liso Vascular/citología , Nicotina/toxicidad , Nitratos/metabolismo , Nitritos/metabolismo , PorcinosRESUMEN
Nitric oxide (NO), which is known to inhibit systemic vascular smooth muscle cell proliferation, is used in the management of neonatal pulmonary hypertension. Our objectives were to determine: (1) if endogenous NO production by neonatal porcine pulmonary artery smooth muscle cells (PASMCs) varied with oxygen tension in vitro, and (2) the effect of exogenous NO and inducible NO synthase (iNOS) stimulators and inhibitors on PASMC proliferation and apoptosis. PASMCs were exposed to different conditions (varying PO(2), NO donors and scavengers, iNOS stimulators and inhibitors) and proliferation, apoptosis, and cyclic guanosine 5(')-monophosphate (cGMP) assessed. PASMCs proliferated best between 5 and 10% O(2) but cGMP levels were similar at all oxygen levels. NO donors (S-nitroso-N-acetyl-penicillamine, NOC-12, NOC-18) inhibited PASMC proliferation in a dose-dependent manner with associated cGMP increases, while NO scavengers (carboxy-PTIO), iNOS stimulators (interleukin-1beta, lipopolysaccharide), and iNOS inhibitors (aminoethylisothiourea) did not affect proliferation or cGMP. No changes in apoptosis were found at the concentrations of NO donors or iNOS stimulators used. These results suggest that while exogenous NO inhibits PASMC proliferation, endogenous NO may not regulate proliferation during changes in oxygen tension or cytokine levels. Endothelial derived and inhaled NO may attenuate smooth muscle hyperplasia and vascular remodeling. Inducible NOS in porcine PASMCs appears resistant to stimulation with interleukin-1beta or lipopolysaccharide. The mechanisms underlying hypoxia-mediated changes in PASMC proliferation require investigation.
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Animales Recién Nacidos/fisiología , Músculo Liso Vascular/citología , Óxido Nítrico/fisiología , Porcinos/fisiología , Animales , Apoptosis/fisiología , División Celular/fisiología , Células Cultivadas , GMP Cíclico/análisis , Daño del ADN , Ensayo de Inmunoadsorción Enzimática , Regulación del Desarrollo de la Expresión Génica , Inmunohistoquímica , Interleucina-1/farmacología , Lipopolisacáridos/farmacología , Músculo Liso Vascular/fisiología , Nitratos/análisis , Óxido Nítrico/biosíntesis , Donantes de Óxido Nítrico/farmacología , Óxido Nítrico Sintasa/fisiología , Óxido Nítrico Sintasa de Tipo II , Compuestos Nitrosos/farmacología , Oxígeno/fisiología , Penicilamina/análogos & derivados , Penicilamina/farmacología , Inhibidores de Fosfodiesterasa/farmacología , Arteria Pulmonar/citología , Arteria Pulmonar/fisiología , Purinonas/farmacologíaRESUMEN
Peptide growth factors are involved in hypoxia-mediated neonatal pulmonary vascular remodeling. The role of hypoxia in the release of selected peptide growth factors from neonatal porcine pulmonary artery smooth muscle cells (PASMC) was examined. PASMC were exposed to different oxygen tensions and the cells were counted electronically and the conditioned media analyzed for basic fibroblast growth factor (bFGF), endothelin-1 (ET-1), platelet-derived growth factor (PDGF), and vascular endothelial growth factor (VEGF). The effect of conditioned media on PASMC proliferation was also measured. Hypoxia (1% oxygen) and hypoxia-conditioned media increased PASMC numbers, and this mitogenic effect was abolished by anti-bFGF, but not by anti-PDGF or anti-VEGF. Hpyoxia increased bFGF and VEGF release but not PDGF or ET-1. This suggests that PASMC-derived bFGF and VEGF may participate in hypoxic neonatal pulmonary vascular remodeling.
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Animales Recién Nacidos/fisiología , Sustancias de Crecimiento/metabolismo , Hipoxia/veterinaria , Músculo Liso Vascular/fisiopatología , Porcinos/fisiología , Animales , Western Blotting/veterinaria , Recuento de Células/veterinaria , División Celular , Células Cultivadas , Medios de Cultivo Condicionados , Factores de Crecimiento Endotelial/análisis , Factores de Crecimiento Endotelial/metabolismo , Endotelina-1/análisis , Endotelina-1/metabolismo , Ensayo de Inmunoadsorción Enzimática/veterinaria , Factor 2 de Crecimiento de Fibroblastos/análisis , Factor 2 de Crecimiento de Fibroblastos/metabolismo , Sustancias de Crecimiento/análisis , Hipoxia/fisiopatología , L-Lactato Deshidrogenasa/análisis , Linfocinas/análisis , Linfocinas/metabolismo , Músculo Liso Vascular/metabolismo , Factor de Crecimiento Derivado de Plaquetas/análisis , Factor de Crecimiento Derivado de Plaquetas/metabolismo , Arteria Pulmonar/metabolismo , Arteria Pulmonar/fisiopatología , Azul de Tripano/química , Factor A de Crecimiento Endotelial Vascular , Factores de Crecimiento Endotelial VascularRESUMEN
When liming running waters, dosers must compensate for different flow and water qualities and for the downstream inflow from acid tributaries which creates mixing zones. At a certain point in the mixing zone, a constant or fluctuating chemical disequilibrium will appear due to transformation processes. In laboratory assays, over-saturated solutions of aluminium with ongoing active precipitation of aluminium have been found to be especially toxic to fish. Recent experiments in a mixing zone in the limed River Audna, Norway, have confirmed this phenomenon. Atlantic salmon (Salmo salar L.) and sea trout (Salmo trutta L.) smolts were exposed to acid and limed waters and mixtures of the two waters downstream from the point of connection. In the acid tributary (mean values: pH=4.8, Ca=1.3 mg litre (-1)), Ali 236 microg litre(-1)=), LT5) was 22 and 40 h for Atlantic salmon and sea trout, respectively. In the mixing zone (pH=4.8-6.5, Ca=1.2-3.2 mg litre(-1), Ali=50-240 microg litre(-1)), LT50 was 7 h for both species, masking the normal species difference in tolerance. Osmoregulatory failure and rapid gill lesions occurred in the mixing zone as an effect of the transformation of Al into high molecular weight precipitating species. This is the first documentation of the existence of such highly toxic mixing zones in nature, and the results clearly show that the mixing zone is even more toxic to fish than acid aluminium-rich waters.
RESUMEN
Eight cases of histologically proved cortical carcinoma are reviewed and compared with several series from the literature. Emphasis is given to defining endocrinologic function in these tumors and to the role of nonoperative treatments. Whereas surgical therapy offered significant demonstrated in any of the patients.