RESUMEN
Results of the study on adaptive immunity in patients with polypous rhinosinusitis (PRS) proved to depend on the degree of eosinophilia in the peripheral blood. The patients were allocated to two groups, one comprised of those having up to 150 eosinophils per 1 microliter the other of the patients with a higher eosinophil concentration. Patients of the former group had a significantly reduced number of CD3+, CD4+, CD8+, and CD20+cells in the peripheral blood that may indicate the necessity of administering immunotropic agents. The opposite picture is characteristic of the latter group in which a rise in the number of the above cells is associated with the increased amount of IgG- and IgA-positive cells. In this situation, the use of systemic immunotropic agents should be restricted. It is concluded that evaluation of systemic and local adaptive immunity is of importance for the choice of an adequate strategy for the treatment of patients with polypous rhinosinusitis.
Asunto(s)
Inmunidad Adaptativa , Eosinofilia/inmunología , Eosinófilos/inmunología , Pólipos Nasales/inmunología , Sinusitis/inmunología , Linfocitos T/inmunología , Adulto , Anciano , Antígenos CD20/inmunología , Complejo CD3/inmunología , Antígenos CD4/inmunología , Antígenos CD8/inmunología , Eosinofilia/sangre , Femenino , Humanos , Inmunoglobulina A/análisis , Inmunoglobulina A/inmunología , Inmunoglobulina G/análisis , Inmunoglobulina G/inmunología , Masculino , Persona de Mediana Edad , Pólipos Nasales/sangre , Sinusitis/sangreRESUMEN
This work was designed to study expression of Toll 1-10 receptors on the surface of cells present in inflammatory infiltrate from nasal polyps and peripheral blood of the patients with polypous rhinosinusitis. It was shown that the intensity of expression depended on the pathomorphological characteristics of nasal polyps. Tissues removed from the patients with polyps of the oedematous type contained more Toll-10 positive cells and showed enhanced expression of Toll-5 receptors on monocytes and lymphocytes, Toll-3 receptors on monocytes, granulocytes, and lymphocytes, and Toll-9 receptors on granulocytes. In contrast, patients with polypous rhinosinusitis and nasal polyps of the fibroedematous type exhibited suppressed expression of Tol-7 receptors on monocytes and Toll-10 receptors on granulocytes coupled to the reduced number of Toll-6 positive lymphocytes as well as enhanced expression of Toll-1 receptors on monocytes, Toll-4 and Toll-5 receptors on granulocytes, and Toll-5 receptors on lymphocytes. It is concluded that only Toll-1, Toll-3, Toll-4, Toll-5, Toll-7, Toll-9 and Toll-10 receptors of their ten types identified thus far in patients with polypous rhinosinusitis and two pathomorphological variants of nasal polyps undergo modulation of expression. These findings open up prospects for the use of new methods for the management of patients with polypous rhinosinusitis by affecting selected components of congenital immunity.
Asunto(s)
Pólipos Nasales/patología , Rinitis/patología , Sinusitis/patología , Receptores Toll-Like/biosíntesis , Femenino , Humanos , Masculino , Pólipos Nasales/inmunología , Rinitis/inmunología , Sinusitis/inmunología , Receptores Toll-Like/análisisRESUMEN
Method of measurement of biological fluids bactericidal activity against Staphylococcus aureus using laser flow cytometry has been developed and proposed for clinical use. Overall bactericidal activity of sera of healthy donors has been assessed by this method. Strong positive correlation between bactericidal activity measured by flow cytometry and ability of the sera of healthy donors to inhibit bacterial growth assessed by photometric method was determined. High degree of positive correlation between results of cytometry and classical microbiological method of measurement of mentioned parameters has been shown.
Asunto(s)
Actividad Bactericida de la Sangre , Donantes de Sangre , Citometría de Flujo/métodos , Infecciones Estafilocócicas/inmunología , Staphylococcus aureus/inmunología , Adolescente , Adulto , Vacunas Bacterianas/inmunología , Humanos , Rayos Láser , Persona de Mediana Edad , Fotometría , Sensibilidad y Especificidad , Staphylococcus aureus/crecimiento & desarrollo , Vacunación , Vacunas Combinadas/inmunologíaRESUMEN
A method for evaluation of serum bactericidal activity against S. aureus by laser flow cytofluorometry has been developed and proposed for clinical application. This method was used to study the overall bactericidal activity of donors' sera and to estimate the contribution of complement to the process of bacteriolysis.