RESUMEN
Three types of heterogeneous preparations and four types of preparations of polysaccharide nature were obtained in studies aimed at the isolation of active compounds from Aspergillus flavus conidia bearing their biological stimulatory activity. Extraction with trichloroacetic acid at 0 degrees C yielded a preparation in which the protein component predominated over the polysaccharide moiety at a ratio of 3 : 1. In the preparation isolated from the phenolic phase of the phenol-water mixture at 68 degrees C the protein polysaccharide ratio was 1 : 1. In the material extracted in the aqueous phase and in that obtained by extraction with acetic acid at 100 degrees C the polysaccharide portion highly predominated (8 : 1 and 7 : 1 respectively).
Asunto(s)
Aspergillus flavus/metabolismo , Polisacáridos/análisis , Aspergillus flavus/crecimiento & desarrollo , Proteínas Fúngicas/análisis , Ácidos Nucleicos/análisis , Polisacáridos/aislamiento & purificaciónRESUMEN
A lipopolysaccharide was isolated by extraction of Aspergillus flavus conidia with 45% phenol at 68-70 degrees C. Quantitative analysis revealed 7% nucleic acids, 5.5% proteins, 46% polysaccharides and 49% liquids, of which 12% were covalently bound. Glucose, mannose, galactose and fucose were detected as monosaccharide components of the polysaccharide moiety by gas chromatography; palmitic acid, stearic acid, oleic acid, linoleic acid and myristic acid were mainly present in the lipidic fraction. This material differs from the bacterial lipopolysaccharides, both in composition of the polysaccharide moiety and representation of fatty acids in the lipidic fraction.