RESUMEN
High levels of propionic acid (PPA) comparable to those of human propionic acidemia were achieved in blood (1-5 mmol/l) and brain (1 micromol/g) of rats by administering saline-buffered propionate (pH 7.4) subcutaneously twice a day from the 6th to the 28th day of life. PPA doses ranged from 1.44 to 1.92 micromol/g body weight as a function of animal age. Control rats were treated with saline in the same volumes. Growth and development of physical landmarks were assessed by monitoring the following parameters daily: body weight, upper incisor eruption, eye opening, and hair coat. Development of some reflexes was also monitored, and a specific subset of motor skills was evaluated at days 14 and 21 of life by the free-fall righting test and the spontaneous alternation test. Chronic PPA administration had no effect on body weight, cerebral cortex weight, or cerebellum weight, but caused slight but significant delays in the day of appearance of hair coat and eye opening, indicating an effect of PPA on the development of physical parameters. Free-fall righting was impaired in PPA-treated animals. On the other hand, PPA administration had no effect on the performance of the animals in the spontaneous alternation tests. Long-term effects of early PPA administration were investigated by assessing animal performance in an aversive task (two-way shuttle avoidance task) and in a nonaversive (open-field task) behavioral task at 60 days of age. PPA-treated rats did not habituate to the open field, and presented a lack of retention of the shuttle-avoidance task. Our results suggest that early postnatal PPA administration to rats alters normal development and induces long-term behavioral deficits in aversive and nonaversive tasks.
Asunto(s)
Acidosis/psicología , Conducta Animal/efectos de los fármacos , Sistema Nervioso/crecimiento & desarrollo , Propionatos/sangre , Envejecimiento/metabolismo , Animales , Reacción de Prevención/efectos de los fármacos , Cerebelo/crecimiento & desarrollo , Cerebelo/metabolismo , Corteza Cerebral/crecimiento & desarrollo , Corteza Cerebral/metabolismo , Femenino , Crecimiento/efectos de los fármacos , Actividad Motora/efectos de los fármacos , Embarazo , Propionatos/farmacocinética , Ratas , Ratas Wistar , Reflejo/efectos de los fármacosRESUMEN
A nine-year-old boy sustained a penetrating injury to the perineum. He later presented with urinary tract infections and a history of passing splinters in the urine. Investigation revealed a foreign body in the urinary bladder, and at suprapubic cystotomy splinters of wood and fragments of cloth were removed.
Asunto(s)
Migración de Cuerpo Extraño/complicaciones , Vejiga Urinaria , Infecciones Urinarias/etiología , Niño , Migración de Cuerpo Extraño/cirugía , Humanos , Masculino , Infecciones Urinarias/cirugíaRESUMEN
A nine-year-old boy sustained a penetrating injury to the perineum. He later presented with urinary tract infections and a history of passing splinters in the urine. Investigation revealed a foreign body in the urinary bladder, and at suprapubic cystotomy splinters of wood and fragments of cloth were removed. (AU)
Asunto(s)
Humanos , Niño , Masculino , Cuerpos Extraños , Heridas Penetrantes/complicaciones , Vejiga Urinaria , Infecciones Urinarias/etiologíaRESUMEN
A nine-year-old boy sustained a penetrating injury to the perineum. He later presented with urinary tract infections and a history of passing splinters in the urine. Investigation revealed a foreign body in the urinary bladder, and at suprapubic cystotomy splinters of wood and fragments of cloth were removed.