RESUMEN
The clinical data of 61 patients of salivary duct carcinoma admitted to the First Medical Centre of Chinese PLA General Hospital from January 2010 to December 2020 were retrospectively reviewed. A total of 55 patients (90.2%) were male and 6 (9.8%) were female. There were 51 patients (83.6%) aged≥50 years. The primary tumor of 45 patients (73.8%) were from the parotid gland. There were 35 patients (57.4%) who had cervical lymph node metastasis and 25 patients (41.0%) had distant metastasis. All patients underwent surgery and 50 of them (82.0%) received adjuvant radiotherapy. The 3-year and 5-year survival rates were 58.0% and 43.3%, respectively. Compared with the patients who had undergone surgery only, the survival rates of those who had postoperative adjuvant radiotherapy and chemoradiotherapy were higher. It can be seen that radical surgical treatment is necessary, and postoperative radiotherapy can reduce the recurrence rate and increase the survival rate to a certain extent.
Asunto(s)
Carcinoma , Neoplasias de las Glándulas Salivales , Carcinoma/patología , Femenino , Humanos , Masculino , Recurrencia Local de Neoplasia/patología , Estadificación de Neoplasias , Pronóstico , Radioterapia Adyuvante , Estudios Retrospectivos , Conductos Salivales/patología , Neoplasias de las Glándulas Salivales/patología , Neoplasias de las Glándulas Salivales/terapia , Tasa de SupervivenciaRESUMEN
OBJECTIVE: Atherosclerosis (AS) is a leading disease with high mortality and morbidity in the world. It has been demonstrated that exosomes can transfer some miRNAs or proteins to regulate the biological functions of human vascular smooth muscle cells (VSMCs) and promote the progression of AS. In this study, we mainly aimed at exploring potential functions of exosomes derived from ox-LDL exposed macrophages and investigating the potential mechanisms of exosome-mediated miR-106a-3p in regulating VSMCs and promoting AS. MATERIALS AND METHODS: Ox-LDL was used to treat THP-1 macrophages, CCK-8 assay was performed to detect cell viability, and flow cytometric analysis was used to detect cell apoptosis. Exosomes were isolated and collected with centrifugation, and were determined by transmission electron microscopy and WB assay. RT-PCR was used to detect the expressions of miRNAs in exosomes and VSMCs, WB assay was used to detect protein expressions. MiR-106a-3p mimic was transfected into VSMCs to verify its functions and the Luciferase gene reporter assay was performed to prove the binding site of miR-106a-3p and CASP9. Finally, GW4869, an inhibitor for exosome secretion, was used to block exosome secretion by ox-LDL induced THP-1 and to confirm the effects of miR-106a-3p on cell proliferation and apoptosis in VSMCs. RESULTS: We found that ox-LDL induced THP-1 could promote cell proliferation and repress cell apoptosis of VSMCs, then, exosomes were successfully isolated, which could promote cell proliferation and repressed cell apoptosis of VSMCs after adding into VSMCs. Furthermore, we found that miR-106a-3p was significantly increased in exosomes from ox-LDL induced THP-1 and its expression was also increased in VSMCs after adding into VSMCs. Moreover, miR-106a-3p overexpression could promote cell viability and repress cell apoptosis, as well as regulate associated protein expressions. Additionally, the Luciferase gene reporter assay confirmed that miR-106a-3p could directly bind with CASP9 and regulate Caspase signaling in VSMCs. Finally, blocking exosomes from ox-LDL induced THP-1 reduced the cell viability and promoted cell apoptosis in VSMCs. CONCLUSIONS: Above all, this study demonstrated that miR-106a-3p was increased in exosomes from ox-LDL induced THP-1 and it could promote cell proliferation and repress cell apoptosis of VSMCs. We found that the exosomes-mediated miR-106a-3p could directly bind with CASP9 and repress Caspase signaling pathway in VSMCs, which might provide a potential target for treating AS.
Asunto(s)
Apoptosis , Exosomas/metabolismo , Lipoproteínas LDL/metabolismo , Macrófagos/metabolismo , MicroARNs/metabolismo , Músculo Liso Vascular/metabolismo , Proliferación Celular , Células Cultivadas , Humanos , MicroARNs/genéticaRESUMEN
OBJECTIVE: To investigate the role of TGF-ß1 on epithelial mesenchymal transition (EMT) and invasion in oral squamous cell carcinoma cell line Tca8113. MATERIALS AND METHODS: Cultured Tca8113 cells were treated with different concentrations of TGF-ß1 for 24 h. The morphological changes were observed under phase-contrast microscopy. The mRNA and protein expression levels of EMT relative marker E-cadherin and Vimentin were detected by RT-PCR and Western blot. The effect of TGF-ß1 on migration and invasion ability of Tca8113 cells were detected using transwell method. RESULTS: The results demonstrated that TGF-ß1 could induce morphological changes in Tca8113 cells from epithelial to mesenchymal. The mRNA and protein level of epithelial marker E-cadherin was downregulated following treatment with TGF-ß1, whereas the mRNA and protein expression level of mesenchymal marker protein Vimentin was upregulated. Furthermore, TGF-ß1 significantly enhances the migration and invasiveness of Tca8113 cells, which were effectively reversed by TGF-ß1 inhibitor, LY2109761 CONCLUSIONS: TGF-ß1 enhances Tca8113 cells migration and invasion by inducing epithelial mesenchymal transition.
Asunto(s)
Carcinoma de Células Escamosas/patología , Transición Epitelial-Mesenquimal/fisiología , Neoplasias de la Boca/patología , Factor de Crecimiento Transformador beta1/metabolismo , Cadherinas/metabolismo , Línea Celular Tumoral , Células Epiteliales/patología , HumanosRESUMEN
We report an unusual case of Burkitt's lymphoma (BL) presenting with jaw lesions in a 14-year-old Chinese boy. The patient presented initially with mobile teeth in all 4 jaw quadrants, with corresponding radiographic detection of alveolar bone crest destruction and periapical bone resorption in the absence of clinically detectable jaw tumors. Moreover, radiographs taken only 17 days later showed clearly distinguishable signs of more extensive alveolar bone destruction compared with the initial radiographs.