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Postpartum pubic symphysis diastasis (PSD) refers to an abnormal widening of the pubic symphysis after delivery. It is a rare and underdiagnosed condition that causes severe pain and can be complicated by osteomyelitis and abscess formation. This article reports a case of a para 4 woman in her 30s with postpartum PSD after vaginal delivery who presented with a prepubic abscess. Multimodal treatment consisted of incision and drainage, physiotherapy and prolonged antibiotic treatment.
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Osteomielitis , Diástasis de la Sínfisis Pubiana , Sínfisis Pubiana , Femenino , Humanos , Absceso/complicaciones , Osteomielitis/complicaciones , Osteomielitis/diagnóstico , Periodo Posparto , Diástasis de la Sínfisis Pubiana/diagnóstico por imagen , Diástasis de la Sínfisis Pubiana/cirugía , AdultoRESUMEN
BACKGROUND: Dilated cardiomyopathy (DCM) is the result of maladaptive cardiac remodeling, which involves microRNA regulation. In turn, microRNAs can contribute to the remodeling process by post-transcriptional modulation of gene expression networks. The exact role of microRNAs in the pathogenesis of DCM is largely unknown. Here, we used an inducible DCM mouse model that carries a human truncation mutation in the sarcomeric protein titin to dissect microRNA pathways in DCM development. METHODS AND RESULTS: MicroRNA microarray studies revealed up-regulation of microRNA-208b in the myocardium of DCM mice and DCM patients (p<0.05 compared to controls). In order to investigate the effect of microRNA-208b on cardiac remodeling, loss-of-function and gain-of-function studies were performed by repetitive injections of LNA-modified microRNA-208b mimics and antimiR-208b. MiR-208b overexpression resulted in cardiac hypertrophy, whereas miR-208b antagonisation prevented transition of adaptive to maladaptive remodeling in the DCM mouse model. In vitro studies identified several pro-hypertrophic transcription factors as potential targets of miR-208b, suggesting that microRNA-208b plays an important role in cardiac development and growth. MiR-208b was also upregulated in DCM patients, but not in heart failure patients due to ischemic heart disease or myocarditis. CONCLUSION: Our data suggests that miR-208b is involved in the remodeling process and pathogenesis of DCM by post-transcriptional gene expression modulation. MicroRNA-208b might be a novel therapeutic target for DCM.
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Cardiomiopatía Dilatada/genética , Regulación de la Expresión Génica , MicroARNs/genética , ARN/genética , Función Ventricular Izquierda/fisiología , Animales , Cardiomiopatía Dilatada/metabolismo , Cardiomiopatía Dilatada/fisiopatología , Línea Celular , Conectina/genética , Humanos , Masculino , Ratones , Ratones Noqueados , MicroARNs/antagonistas & inhibidores , MicroARNs/biosíntesis , Mutación , Miocardio/metabolismo , Miocardio/patología , Reacción en Cadena de la Polimerasa de Transcriptasa InversaRESUMEN
BACKGROUND: In neural invasion (NI), cancer cells are classically assumed to actively invade nerves and to cause local recurrence and pain. However, the opposite possibility, that nerves may reach cancer cells even in their preinvasive stage and thereby promote cancer spread, has not yet been genuinely considered. The present study analyzes the reaction of Schwann cells of peripheral nerves to carcinogenesis in pancreatic cancer and colon cancer. METHODS: Two novel 3D migration and Schwann cell outgrowth assays were developed to monitor the timing and the specificity of Schwann cell migration and cancer invasion toward peripheral neurons through digital-time-lapse microscopy and after blockade of nerve growth factor (NGF) signalling via siRNA or a small-molecule inhibitor of the p75(NTR) receptor. The frequency and emergence of the Schwann cell markers Sox10, S100, ALDH1L1, and glial-fibrillary-acidic-protein (GFAP) around cancer precursor lesions were studied in human and conditional murine pancreatic and colon cancer specimens using multiple immunolabeling. RESULTS: Schwann cells migrated toward pancreatic and colon cancer cells, but not toward benign cells, before the onset of cancer migration toward peripheral neurons. This chemoattraction was inhibited after blockade of p75(NTR)-signaling on Schwann and pancreatic cancer cells. Schwann cells were specifically detected around murine and human pancreatic intraepithelial neoplasias (PanINs) (mean percent of murine PanINs surrounded by Schwann cells = 78.9%, 95% CI = 70.9 to 86.8%, and mean percent of human PanINs surrounded by Schwann cells = 52.5%, 95% CI = 14.7 to 90.4%; human: n = 44, murine: n = 14) and intestinal adenomas (mean percent of murine adenomas surrounded by Schwann cells = 64.2%, 95% CI = 28.6 to 99.8%, and mean percent of human adenomas surrounded by Schwann cells = 17.2%, 95% CI = -126.9 to 161.4; human: n = 36, murine: n = 12). The Schwann cell presence in this premalignant stage was associated with the frequency of NI in the malignant phase. CONCLUSIONS: Schwann cells have particular and specific affinity to cancer cells. Emergence of Schwann cells in the premalignant phase of pancreatic and colon cancer implies that, in contrast with the traditional assumption, nerves-and not cancer cells-migrate first during NI.
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Movimiento Celular , Neoplasias del Colon/patología , Neoplasias Pancreáticas/patología , Lesiones Precancerosas/patología , Células de Schwann/patología , Aldehído Deshidrogenasa/metabolismo , Familia de Aldehído Deshidrogenasa 1 , Animales , Neoplasias del Colon/metabolismo , Proteína Ácida Fibrilar de la Glía , Humanos , Inmunohistoquímica , Isoenzimas/metabolismo , Ratones , Invasividad Neoplásica , Proteínas del Tejido Nervioso/metabolismo , Oxidorreductasas actuantes sobre Donantes de Grupo CH-NH , Neoplasias Pancreáticas/metabolismo , Lesiones Precancerosas/metabolismo , Retinal-Deshidrogenasa/metabolismo , Proteínas S100/metabolismo , Factores de Transcripción SOXE/metabolismo , Células de Schwann/metabolismoRESUMEN
AIM: To investigate molecular phenotypes of myocardial B19V-infection to determine the role of B19V in myocarditis and dilated cardiomyopathy (DCM). METHODS: Endomyocardial biopsies (EMBs) from 498 B19V-positive patients with myocarditis and DCM were analyzed using molecular methods and functional experiments. EMBs were obtained from the University Hospitals of Greifswald and Tuebingen and additionally from 36 German cardiology centers. Control tissues were obtained at autopsy from 34 victims of accidents, crime or suicide. Identification of mononuclear cell infiltrates in EMBs was performed using immunohistological staining. Anti-B19V-IgM and anti-B19V-IgG were analyzed by enzyme-linked immunosorbent assay (ELISA). B19V viral loads were determined using in-house quantitative real-time polymerase chain reaction (PCR). For B19V-genotyping a new B19V-genotype-specific restriction fragment length polymorphism (RFLP)-PCR was established. B19V-genotyping was verified by direct DNA-sequencing and sequences were aligned using BLAST and BioEdit software. B19V P6-promoter and HHV6-U94-transactivator constructs were generated for cell culture experiments. Transfection experiments were conducted using human endothelial cells 1. Luciferase reporter assays were performed to determine B19V-replication activity. Statistical analysis and graphical representation were calculated using SPSS and Prism5 software. RESULTS: The prevalence of B19V was significantly more likely to be associated with inflammatory cardiomyopathy (iCMP) compared to uninflamed DCM (59.6% vs 35.3%) (P < 0.0001). The detection of B19V-mRNA replication intermediates proved that replication of B19V was present. RFLP-PCR assays showed that B19V-genotype 1 (57.4%) and B19V-genotype 2 (36.7%) were the most prevalent viral genotypes. B19V-genotype 2 was observed more frequently in EMBs with iCMP (65.0%) compared to DCM (35%) (P = 0.049). Although there was no significant difference in gender-specific B19V-loads, women were more frequently infected with B19V-genotype 2 (44.6%) than men (36.0%) (P = 0.0448). Coinfection with B19V and other cardiotropic viruses was found in 19.2% of tissue samples and was associated with higher B19V viral load compared to B19V-monoinfected tissue (P = 0.0012). The most frequent coinfecting virus was human herpes virus 6 (HHV6, 16.5%). B19V-coinfection with HHV6 showed higher B19V-loads compared to B19V-monoinfected EMBs (P = 0.0033), suggesting that HHV6 had transactivated B19V. In vitro experiments confirmed a 2.4-fold increased B19V P6-promoter activity by the HHV6 U94-transactivator. CONCLUSION: The finding of significantly increased B19V loads in patients with histologically proven cardiac inflammation suggests a crucial role of B19V-genotypes and reactivation of B19V-infection by HHV6-coinfection in B19V-associated iCMP. Our findings suggest that B19V-infection of the human heart can be a causative event for the development of an endothelial cell-mediated inflammatory disease and that this is related to both viral load and genotype.
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In patients with acute myocarditis, viral genome can be detected in plasma and peripheral leukocytes. Its relationship with active myocardial inflammation, however, is not well understood. Myocardial edema as a feature of inflammation and myocardial necrosis or fibrosis can be frequently observed in patients with acute myocarditis by cardiovascular magnetic resonance (CMR). We assessed the association of viral genome presence in peripheral blood samples with myocardial edema and irreversible injury. We examined consecutive patients with clinically suspected myocarditis after an episode of viral illness. State-of-the-art methods were used for detecting myocardial edema and irreversible injury using CMR and viral genome applying reverse transcribed, nested polymerase chain reaction in peripheral blood samples. The specificity of viral amplification products was confirmed by automatic DNA sequencing. Of a total of 55 patients (53.5 ± 15.6 years), 21 were positive for viral genome in peripheral leukocytes. Interestingly, 18 (86%) of these patients also showed global myocardial edema, as compared to only 7/34 (21%) without PCR evidence for viral genome. The overall agreement between CMR criteria for edema and viral PCR was 84%. In contrast, there was no significant relationship of viral genome presence with myocardial necrosis or scars. In patients with clinically suspected myocarditis, myocardial edema but not irreversible myocardial injury is associated with the presence of viral genome in peripheral blood.
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ADN Viral/sangre , Edema Cardíaco/diagnóstico , Genoma Viral , Imagen por Resonancia Magnética , Miocarditis/diagnóstico , Miocardio/patología , Reacción en Cadena de la Polimerasa de Transcriptasa Inversa , Virosis/diagnóstico , Adulto , Anciano , Automatización de Laboratorios , Distribución de Chi-Cuadrado , Medios de Contraste , Edema Cardíaco/sangre , Edema Cardíaco/tratamiento farmacológico , Edema Cardíaco/patología , Edema Cardíaco/fisiopatología , Edema Cardíaco/virología , Femenino , Fibrosis , Humanos , Masculino , Persona de Mediana Edad , Miocarditis/sangre , Miocarditis/tratamiento farmacológico , Miocarditis/patología , Miocarditis/fisiopatología , Miocarditis/virología , Necrosis , Valor Predictivo de las Pruebas , Estudios Prospectivos , Análisis de Secuencia de ADN , Volumen Sistólico , Función Ventricular Izquierda , Virosis/sangre , Virosis/tratamiento farmacológico , Virosis/patología , Virosis/fisiopatología , Virosis/virologíaRESUMEN
Hospital transfers from nursing homes are frequent, costly, often preventable, and can have negative effects on the residents' health. The present study investigated the current situation in Carinthia (Austria) regarding the characteristics of relocated nursing home residents, the proportion of avoidable transfers, the consequences of relocation from the physicians' and nurses' perspectives and ways for improving nursing home care. Retrospectively, the documentations of a regional hospital (N=4149), a rescue service (N=10754), and a social insurance agency (N=7051) were analysed; qualitative interviews with physicians (N=25) and nursing administrators (N=16) were conducted. A considerable proportion of these transports seemed to be avoidable: for example, about 40% of the ambulatory treatments in the emergency department of the investigated hospital were inappropriate. Options for improving the current situation will be discussed.
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Hogares para Ancianos/estadística & datos numéricos , Casas de Salud/estadística & datos numéricos , Readmisión del Paciente/estadística & datos numéricos , Transferencia de Pacientes/estadística & datos numéricos , Procedimientos Innecesarios/estadística & datos numéricos , Anciano , Anciano de 80 o más Años , Actitud del Personal de Salud , Austria , Estudios Transversales , Servicio de Urgencia en Hospital/estadística & datos numéricos , Femenino , Humanos , Masculino , Dinámica Poblacional , Revisión de Utilización de Recursos/estadística & datos numéricosRESUMEN
The main cause of death from novel (swine origin) influenza A/H1N1 infection is acute respiratory distress syndrome. Most fatal cases are immunocompromised patients or patients with a severe underlying disease. Here, we report a fatal case of acute interstitial myocarditis associated with novel influenza A/H1N1 infection in an immunocompetent young woman. A previously healthy 18-year-old woman experienced malaise, diarrhea, and fever for several days prior to a sudden collapse at home. Autopsy revealed a predominantly lymphocytic myocarditis in the absence of a significant respiratory tract infection. Infection with novel (swine origin) influenza A/H1N1 was confirmed by PCR analysis of blood as well as myocardial tissue. Influenza-caused diarrhea with consecutive hypokalemia potentially contributed to the fatal outcome of the myocarditis, characterized by ventricular fibrillation. In conclusion, sudden death by myocarditis may be a rare complication of novel influenza A/H1N1 infection in otherwise healthy individuals, even in the absence of significant respiratory tract infection.
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Muerte Súbita Cardíaca/etiología , Subtipo H1N1 del Virus de la Influenza A/aislamiento & purificación , Gripe Humana/virología , Miocarditis/patología , Adolescente , ADN Viral/análisis , Muerte Súbita Cardíaca/patología , Resultado Fatal , Femenino , Humanos , Inmunocompetencia , Subtipo H1N1 del Virus de la Influenza A/genética , Gripe Humana/patología , Miocarditis/virología , Reacción en Cadena de la PolimerasaRESUMEN
Interdisciplinary collaboration between nurses and physicians contributes to optimal patient outcomes. Both insufficient knowledge of each other's roles and competencies and the power position of physicians impede nurse-physician collaboration. Health care managers play an important role in the promotion of nurse-physician collaboration. Leadership is associated with masculine traits, but female attributes are crucial in social relationships. Austrian health care management students (n=141, response rate: 93 %) rated themselves, the typical nurse and the typical physician with respect to masculine and feminine traits using the Bem sex-role-inventory (BSRI). The respondents saw themselves as equally masculine and feminine (androgynous self-concept); nurses were rated as significantly more masculine than feminine, whereas physicians were described as masculine sex-typed and significantly less feminine than nurses. For health care managers who also have to promote interdisciplinary collaboration an androgynous self-concept can be regarded as advantageous. They need to reflect on their ideas about nurses and physicians in order to manage the challenge of promoting interprofessional co-operation.
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Identidad de Género , Relaciones Interprofesionales , Orientación , Femenino , Humanos , Masculino , Enfermeras y Enfermeros/psicología , Médicos/psicología , AutoimagenRESUMEN
Despite of intensive professionalisation efforts and changed legal frameworks the public image of nursing is still full of gender-stereotypes. This study investigates to what extend public stereotypes of "typically female" and "typically male" are reflected in the self-concept and ideals of nurses. Using a cross-sectional research design 174 female nurses were surveyed regarding their sex-role orientation and ideals (femininity and masculinity) using a standardized questionnaire (BSRI). Contrary to stereotypes the respondents indentified more with masculine traits. Masculine attributes predominated especially in ideas about the ideal nurse. No differences between nurses who work in different areas could be revealed. In all endeavors regarding autonomy and masculinity the significance of the feminine function of "caring" must not be forgotten. Teachers in healthcare and nursing play a central role in sensibilising students for gender issues.
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Identidad de Género , Rol de la Enfermera/psicología , Personal de Enfermería en Hospital/psicología , Autoimagen , Estudios Transversales , Femenino , Alemania , Humanos , Investigación en Enfermería , Inventario de Personalidad/estadística & datos numéricos , Psicometría , EstereotipoRESUMEN
Due to demographic changes and the growing demand for a nursing workforce in the gerontological field adequate nursing curricula are required. This paper describes the development of the international online gerontological master degree programme GEROM. Health and nursing science institutions from six European countries are collaborating in this EU-project. The project milestones as well as the study programme architecture are introduced. Furthermore, the evaluation concept is illustrated. Collaboration across the partners is affected by some problems; varying accreditation procedures and cultural differences inhibit the implementation of a joint degree. Institutional commitment and communication between the partners are essential for a successful process.
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Educación a Distancia , Educación de Postgrado en Enfermería , Enfermería Geriátrica/educación , Cooperación Internacional , Acreditación , Curriculum , Europa (Continente) , Humanos , Sistemas en Línea , Desarrollo de ProgramaRESUMEN
The daily routine of nurses in long term care units is characterized by a variety of physical and psychological burdens. This study is dedicated to work load, burnout, health and coping of this target group. Registered nurses were surveyed in two federal states of Austria, namely in Vienna (n = 78) and Carinthia (n = 59). The individuals of the, on average, older Viennese sample who often feel destined for this occupation face younger colleagues from rural areas whose motivations are predominantly pro-social and economic. The results show an alarming proportion of emotional exhaustion (23%) and aversion to residents (17%). The participants felt particularly burdened by work conditions and the old people they are caring for. Carinthian nurses estimated their state of health to be better than that of their Viennese colleagues. The Viennese sample was found to be significantly more discontent than the Carinthian one. The study underlines the high risk of burnout for nurses in long term care units.