Asunto(s)
Citotoxicidad Inmunológica/efectos de los fármacos , Células Asesinas Naturales/citología , Células Asesinas Naturales/efectos de los fármacos , Pirimidinas/farmacología , Tiazoles/farmacología , Dasatinib , Humanos , Células Jurkat , Células K562 , Leucemia Mielógena Crónica BCR-ABL Positiva/tratamiento farmacológico , Inhibidores de Proteínas Quinasas/farmacología , Inhibidores de Proteínas Quinasas/uso terapéutico , Pirimidinas/uso terapéutico , Receptores de Antígenos de Linfocitos T/metabolismo , Transducción de Señal/efectos de los fármacos , Tiazoles/uso terapéutico , Familia-src Quinasas/antagonistas & inhibidoresRESUMEN
Our previous studies identified a role for MZM in the movement of lymphocytes into the splenic white pulp. Here we show that phagocytosis of colloidal carbon by marginal zone macrophages results in a splenic influx of B lymphocytes, and T lymphocytes of memory/activated phenotype, with concomitant upregulation of B Lymphocyte Chemoattractant (BLC, CXCL13) mRNA, a chemokine acting on B and memory/activated T lymphocytes. The recruitment of B cells and activated T cells to the spleen after phagocytic uptake would allow an immune response against blood-borne pathogens to be quickly and effectively mounted by bringing together the two key cell types responsible for generating humoral immunity.
Asunto(s)
Linfocitos B/inmunología , Quimiotaxis de Leucocito , Macrófagos/inmunología , Fagocitosis , Bazo/inmunología , Linfocitos T/inmunología , Animales , Carbono/sangre , Carbono/inmunología , Quimiocina CXCL13 , Quimiocinas CXC/genética , Quimiocinas CXC/metabolismo , Quimiotaxis de Leucocito/genética , Combinación de Medicamentos , Femenino , Memoria Inmunológica , Ratones , Ratones Endogámicos BALB C , Povidona , ARN Mensajero/análisis , ARN Mensajero/metabolismo , Regulación hacia ArribaRESUMEN
Normal snapper (Pagrus auratus Bloch and Schneider) serum was examined for natural IgM that binds to protease (bromelain) treated sheep erythrocytes (BrSRBC) in a model assay system that has been used to appraise natural IgM of various mammals. Normal snapper serum lysed BrSRBC while haemolysis was abrogated by heat inactivation of serum and in divalent cation-deficient conditions, indicative of classical complement mediated lysis. In addition, heat inactivated normal snapper serum agglutinated BrSRBC while phosphatidylcholine (PtC) liposomes partially inhibited both haemolysis and agglutination. Inhibition of haemolysis and agglutination may have been mediated by an interaction between immunoglobulin (Ig) and PtC as protein A purified snapper Ig bound to PtC liposomes. However it is not known if this binding was PtC specific nor if the binding was initiated by either the Fab and/or Fc domains of snapper Ig. BrSRBC plaque forming cells (PFC) were detected in the peritoneal cavity, spleen, head kidney and peripheral blood of normal snapper. The greatest proportion of BrSRBC PFC per B cell was within the peritoneal cavity followed by the spleen, peripheral blood and head kidney. Together, these data suggest that normal snapper serum may contain natural Ig that binds BrSRBC, activating the classical complement cascade.