RESUMEN
A series of copolymers of acrylamide (Am) with 2-acrylamido-2-methylpropanesulfonic acid (AmMPSA) and N-2-hydroxypropyl-methacrylamide (HPMA) with acrylic acid (AA) have been synthesized. Complexes of gentamycin in the base form with these polymers and dextran sulfate were obtained. It is established that both poly(AmMPCA) and its complex with gentamycin exhibit an antiviral activity in vitro against human A(H3N2) influenza virus and herpes simplex virus of type 1 (HSV-1). The HPMA-AA copolymer and its complex with gentamycin are only active against HSV-1. Complexes of gentamycin with the polyanions based on the Am derivatives and dextran sulfate showed a high level of antibacterial activity against four bacterial strains. All the polymers studied as well as their complexes with gentamycin demonstrated a low toxicity in vitro, which makes them promising for the creation of preparations with combined antiviral and antimicrobial properties.
Asunto(s)
Resinas Acrílicas/farmacología , Alcanosulfonatos/farmacología , Antivirales/farmacología , Gentamicinas/farmacología , Herpes Simple/tratamiento farmacológico , Herpesvirus Humano 1 , Subtipo H3N2 del Virus de la Influenza A , Gripe Humana/tratamiento farmacológico , Aniones , Antibacterianos/farmacología , Línea Celular , Herpes Simple/metabolismo , Humanos , Gripe Humana/metabolismoRESUMEN
In 35 patients with herpes virus infection (males and females aged 25 to 45 years) the content of ceruloplasmin and immunoglobulins of the main classes in the blood sera and the content of IgA in saliva at the stages of exacerbation and remission were evaluated. For control, a group of 35 healthy donors of the same ages were used. In patients with relapsing herpes virus infection even at the period of remission reliably higher levels of ceruloplasmin and immunoglobulins of the main classes were registered in comparison with those in the group of healthy donors. This was indicative of the fact that constant antigenic load caused by virus persistence and, in our opinion, could be regarded as a sign of unfavorable prognosis. At the periods of exacerbation a reliable increased level of secretory IgA was registered in a group of patients with rare relapses of herpes virus infection in comparison with a group of patients with frequent relapses, which showed that patients with rare relapses had a better immune response.
Asunto(s)
Ceruloplasmina/análisis , Herpes Simple/inmunología , Herpes Simple/metabolismo , Inmunoglobulinas/sangre , Adulto , Biomarcadores/análisis , Femenino , Herpes Simple/sangre , Humanos , Inmunoglobulina A/análisis , Masculino , Persona de Mediana Edad , Recurrencia , Saliva/inmunologíaRESUMEN
Clinical isolates of the Herpes simplex virus had different susceptibility to acyclovir. Along with highly susceptible variants there were often isolated resistant variants and variants with intermediate susceptibility to the drug. All the clinical isolates were from the patients previously not treated with acyclovir. Therefore, it is possible to consider the drug resistance of the Herpes simplex virus to be natural. Two cultures of the virus differing in their susceptibility to acyclovir were simultaneously isolated from the affections of various localization in one patient. The study of the time course of the resistance development in the cell cultures showed that it depended on the drug dose and the subculture level. It is advisable to test the isolates of the Herpes simplex virus for their susceptibility to drugs used in the patient treatment. This will provide individual therapy of the patients with Herpes simplex.