RESUMEN

The amino acid sequence of this neuropeptide was elucidated by means of a combined approach of enzymatic digestions, manual and automatic Edman degradations, and mass spectrometry. It is a 72 residue peptide (molecular mass 8388 Da), with six cysteines forming three disulfide bridges connecting residues 7-43, 23-39, and 26-52, with blocked N- and C-termini, and lacking the amino acids histidine, methionine, and tryptophan. The CHH-I of Procambarus bouvieri is compared with the other known CHHs from Orconectes limosus (98.6% identity), Homarus americanus isomorph A (83.3% identity), Homarus americanus isomorph B (79.2% identity), and Carcinus maenas (61.1% identity).

Asunto(s)

Astacoidea/química , Hormonas de Invertebrados/química , Proteínas del Tejido Nervioso/química , Secuencia de Aminoácidos , Animales , Proteínas de Artrópodos , Braquiuros , Cromatografía Líquida de Alta Presión , Hormonas de Invertebrados/aislamiento & purificación , Datos de Secuencia Molecular , Nephropidae , Proteínas del Tejido Nervioso/aislamiento & purificación , Sistemas Neurosecretores/química , Mapeo Peptídico , Homología de Secuencia de Aminoácido , Especificidad de la Especie