RESUMEN
The increasing demand for water, food and energy poses challenges for the world´s sustainability. Tropical palm oil is currently the major source of vegetable oil worldwide with a production that exceeds 55 million tons per year, while generating over 200 million tons of palm oil mill effluent (POME). It could potentially be used as a substrate for production of microalgal biomass though. In this study, the microalgal strain Chlamydomonas biconvexa Embrapa|LBA40, originally isolated from a sugarcane vinasse stabilization pond, was selected among 17 strains tested for growth in POME retrieved from anaerobic ponds of a palm oil industrial plant located within the Amazon rainforest region. During cultivation in POME, C. biconvexa Embrapa|LBA40 biomass productivity reached 190.60 mgDW ⢠L-1 ⢠d-1 using 15L airlift flat plate photobioreactors. Carbohydrates comprised the major fraction of algal biomass (31.96%), while the lipidic fraction reached up to 11.3% of dry mass. Reductions of 99% in ammonium and nitrite, as well as 98% reduction in phosphate present in POME were detected after 5 days of algal cultivation. This suggests that the aerobic pond stage, usually used in palm oil industrial plants to reduce POME inorganic load, could be substituted by high rate photobioreactors, significantly reducing the time and area requirements for wastewater treatment. In addition, the complete mitochondrial genome of C. biconvexa Embrapa|LBA40 strain was sequenced, revealing a compact mitogenome, with 15.98 kb in size, a total of 14 genes, of which 9 are protein coding genes. Phylogenetic analysis confirmed the strain taxonomic status within the Chlamydomonas genus, opening up opportunities for future genetic modification and molecular breeding programs in these species.
Asunto(s)
Chlamydomonas/metabolismo , Microbiología Industrial/métodos , Aceite de Palma/metabolismo , Filogenia , Aguas Residuales/microbiología , Biodegradación Ambiental , Biomasa , Chlamydomonas/clasificación , Chlamydomonas/genética , Genoma MitocondrialRESUMEN
Blood donation is a social practice that helps treat diseases and maintain public health. The DoeSangue application was designed and developed to support donor recruitment and loyalty, strengthening health promotion and social engagement. We aimed to assess the DoeSangue application from the perspective of hematology and hemotherapy experts. A methodological, applied and qualitative research was carried out from September 2015 to July 2017 in Fortaleza, Ceará. The study was based on Participatory Interaction Design associated with Symbolic Interactionism. After conducting the first two steps, application design and development in a laboratory and assessment by donor users, the application was validated by eight experts from the Fortaleza's public blood center. For data collection, the 'application validation form with experts' was used based on a Likert-type scale, and a focus group was conducted. The tool was positively assessed by participants, with an average Content Validation Index of 0.88. Evaluators pointed out, among other features, the tool's ability to promote interactivity, mobilization and social engagement, in addition to contributing to gathering and loyalty of blood donors.
A doação de sangue é uma prática social que auxilia no tratamento de doenças e na manutenção da saúde coletiva. O aplicativo DoeSangue foi concebido e desenvolvido para apoiar a captação e a fidelização de doadores, fortalecendo a promoção da saúde e o engajamento social. Objetivou-se avaliar o aplicativo DoeSangue sob a perspectiva de especialistas das áreas de hematologia e hemoterapia. Realizou-se uma pesquisa metodológica, aplicada e qualitativa, de setembro de 2015 a julho de 2017, em Fortaleza, Ceará. O estudo fundamentou-se no Design de Interação Participativo associado ao Interacionismo Simbólico. Após as duas primeiras etapas - concepção e desenvolvimento do aplicativo em laboratório, e avaliação pelos usuários doadores - o aplicativo foi validado por oito especialistas do hemocentro público de Fortaleza. Para a coleta de dados, utilizou-se o 'formulário de validação do aplicativo com especialistas', com base na escala de Likert, e realizou-se um grupo focal. A ferramenta foi avaliada positivamente pelos participantes, com Índice de Validação de Conteúdo médio de 0,88. Os avaliadores apontaram, dentre outras funcionalidades, a capacidade da ferramenta em promover interatividade, mobilização e engajamento social, além de contribuir com a captação e a fidelização de doadores de sangue.
Asunto(s)
Donantes de Sangre , Promoción de la Salud , Brasil , Grupos Focales , Humanos , Investigación CualitativaRESUMEN
Resumo A doação de sangue é uma prática social que auxilia no tratamento de doenças e na manutenção da saúde coletiva. O aplicativo DoeSangue foi concebido e desenvolvido para apoiar a captação e a fidelização de doadores, fortalecendo a promoção da saúde e o engajamento social. Objetivou-se avaliar o aplicativo DoeSangue sob a perspectiva de especialistas das áreas de hematologia e hemoterapia. Realizou-se uma pesquisa metodológica, aplicada e qualitativa, de setembro de 2015 a julho de 2017, em Fortaleza, Ceará. O estudo fundamentou-se no Design de Interação Participativo associado ao Interacionismo Simbólico. Após as duas primeiras etapas - concepção e desenvolvimento do aplicativo em laboratório, e avaliação pelos usuários doadores - o aplicativo foi validado por oito especialistas do hemocentro público de Fortaleza. Para a coleta de dados, utilizou-se o 'formulário de validação do aplicativo com especialistas', com base na escala de Likert, e realizou-se um grupo focal. A ferramenta foi avaliada positivamente pelos participantes, com Índice de Validação de Conteúdo médio de 0,88. Os avaliadores apontaram, dentre outras funcionalidades, a capacidade da ferramenta em promover interatividade, mobilização e engajamento social, além de contribuir com a captação e a fidelização de doadores de sangue.
Abstract Blood donation is a social practice that helps treat diseases and maintain public health. The DoeSangue application was designed and developed to support donor recruitment and loyalty, strengthening health promotion and social engagement. We aimed to assess the DoeSangue application from the perspective of hematology and hemotherapy experts. A methodological, applied and qualitative research was carried out from September 2015 to July 2017 in Fortaleza, Ceará. The study was based on Participatory Interaction Design associated with Symbolic Interactionism. After conducting the first two steps, application design and development in a laboratory and assessment by donor users, the application was validated by eight experts from the Fortaleza's public blood center. For data collection, the 'application validation form with experts' was used based on a Likert-type scale, and a focus group was conducted. The tool was positively assessed by participants, with an average Content Validation Index of 0.88. Evaluators pointed out, among other features, the tool's ability to promote interactivity, mobilization and social engagement, in addition to contributing to gathering and loyalty of blood donors.
Asunto(s)
Humanos , Donantes de Sangre , Promoción de la Salud , Brasil , Grupos Focales , Investigación CualitativaRESUMEN
BACKGROUND: Omega fatty acids are a family of polyunsaturated fats associated with several health benefits. Lipases are enzymes with potential application in several food processes such as flavor and aroma, surfactants and formulations for the dairy and bakery industries. In this study, single cell oil and lipase production by Candida viswanathii CCR8137 were evaluated simultaneously from renewable carbon sources under nitrogen limitation. METHODS: Enzyme and single cell oil were obtained in submerged cultivations supplemented with triolein, tributyrin, corn oil, sunflower oil, canola oil and olive oil. The effects of glucose on lipid accumulation, fatty acid profile, enzyme production and cell morphology were also evaluated. RESULTS: The highest lipid accumulation (44.5%, w/w) was obtained from triolein, whereas olive oil was the best inducer of lipase synthesis (26.8 U/mL). Nitrogen limiting cultivations were a key parameter for an organic source which showed higher lipid accumulation and enzyme production than the tested inorganic nitrogen source. Glucose was a poor inducer of lipase synthesis, though increased values of lipid accumulation were observed from this carbon source with a maximum of 63.1% (w/w). The fatty acid profile of lipids produced by C. viswanathii CCR8137 showed a high content of omega-9 fatty acid (C18:1 n-9). The addition of glucose to the culture media resulted in the synthesis of essential fatty acids: vaccenic, linolenic and eicosadienoic acids. CONCLUSIONS: Therefore, C. viswanathii CCR8137 strain can be considered as an oleaginous yeast able to accumulate high concentrations of intracellular lipids, which are potential additives for food industry applications as well as being able to simultaneously synthesize high yields of lipase.
Asunto(s)
Candida/metabolismo , Glucosa/farmacología , Lipasa/metabolismo , Aceites de Plantas/farmacología , Triglicéridos/farmacología , Trioleína/farmacología , Glucosa/metabolismo , Metabolismo de los Lípidos , Aceites de Plantas/metabolismo , Análisis de la Célula Individual , Triglicéridos/metabolismo , Trioleína/metabolismoRESUMEN
Standard diagnostic methods currently in use for the identification of helminth infections in ruminants are based on the morphological analysis of immature and adult stages of parasites. This paper describes a method for the semiquantitative identification of nematodes, mainly Trichostrongyloidea, at species-level resolution. The method is based on amplification and fragment analysis followed by minisequencing of the ITS-2 region (internal transcribed spacer 2) of the ribosomal DNA of parasite eggs or larvae. This method allows for the identification of seven genera (Chabertia, Cooperia, Haemonchus, Oesophagostomum, Ostertagia, Teladorsagia, and Trichostrongylus) and 12 species (Chabertia ovina, Cooperia curticei, Cooperia punctata, Cooperia oncophora/Cooperia surnabada, Haemonchus contortus, Haemonchus placei, Haemonchus longistipes, Oesophagostomum asperum, Oesophagostomum radiatum, Ostertagia ostertagi, Trichostrongylus axei, and Trichostrongylus colubriformis) of infectious nematodes of domestic ruminants. The concordance between the morphological and molecular analyses in the detection of genera ranged from 0.84 to 0.99, suggesting the proposed detection method is specific, semiquantitative, less laborious, and highly cost-efficient.
Asunto(s)
Infecciones por Nematodos/veterinaria , Rumiantes/parasitología , Trichostrongyloidea/aislamiento & purificación , Animales , Bovinos , Enfermedades de los Bovinos/parasitología , ADN de Helmintos , ADN Ribosómico , Cabras , Haemonchus/genética , Haemonchus/aislamiento & purificación , Infecciones por Nematodos/parasitología , Técnicas de Amplificación de Ácido Nucleico/veterinaria , Oesophagostomum/genética , Oesophagostomum/aislamiento & purificación , Ostertagia/genética , Ostertagia/aislamiento & purificación , Ovinos , Strongyloidea/genética , Trichostrongyloidea/genética , Trichostrongylus/genéticaRESUMEN
The neuromuscular adaptations between ergometer-based high-intensity interval training (HIIT-T; n = 15), whole-body high-intensity interval training (HIIT-WB; n = 12) and moderate-intensity continuous training (MICT; n = 14) were compared in forty-one healthy men randomized to 16 weeks of training (3x per week). Two-way repeated measures analysis of variance (ANOVA) showed countermovement (CMJ) and squat (SJ) jump height (HIIT-T: 8.5 ± 13.3%; 3.1 ± 9.7%, HIIT-WB: 6.4 ± 9.8%, 10.4 ± 16.1% and MICT: 2.2 ± 9.5%; 4.4 ± 12.1%, respectively), SJ peak power (HIIT-T: 1.7 ± 3.9%; HIIT-WB : 6.4 ± 7.9%; MICT: 0.5 ± 6.5%) and CMJ rate of force development (HIIT-T: 58.1 ± 50.5%; HIIT-WB: 36.9 ± 54.2%; MICT: 38.4 ± 64.3%) improved similarly in all training groups (all p < 0.05). CMJ peak power increased only after HIIT-T (4.3 ± 5.5%) and HIIT-WB (4.5 ± 5.2%), while no differences were observed in both the rectus femoris and vastus lateralis maximal electromyographic amplitude. Finally, marked improvements were also observed in the number of repetitions in the HIIT-WB protocol at the eighth week, with no further improvement at the sixteenth week. These data suggest that 16 weeks of HIIT-WB is capable to improve neuromuscular function to a similar extent as HIIT-T and MICT.
Asunto(s)
Adaptación Fisiológica , Entrenamiento de Intervalos de Alta Intensidad/métodos , Músculo Esquelético/fisiología , Acondicionamiento Físico Humano/métodos , Electromiografía , Prueba de Esfuerzo/métodos , Humanos , Contracción Isométrica , Masculino , Ejercicio Pliométrico , Adulto JovenRESUMEN
Evolution has equipped poxvirus genomes with the coding capacity for several virus-host interaction products which interfere with host cell gene expression and protein function, creating an adequate intracellular environment for a productive infection. We show here that Vaccinia virus (VACV) induces the expression of the cellular transcription factor EGR-1 (early growth response-1) in Mouse Embryonic Fibroblasts (MEFs) through the MEK (mitogen-activated protein kinase (MAPK)/ERK)/ERK (extracellular signal-regulated kinases) pathway, from 3 to 12 h post infection (h.p.i.). By using starved egr-1 knockout (egr-1-/-) MEFs, we demonstrate that VACV replication is reduced by ~1 log in this cell line. Although western blotting and electron microscopy analyses revealed no difference in VACV gene expression or morphogenesis, the specific infectivity of VACV propagated in egr-1-/- MEFs was lower than virus propagated in wild type (WT) cells. This lower infectivity was due to decreased VACV DNA replication during the next cycle of infection. Taken together, these results revealed that EGR-1 appears to facilitate VACV replication in starved fibroblasts by affecting viral particles infectivity.
Asunto(s)
Proteína 1 de la Respuesta de Crecimiento Precoz/genética , Regulación de la Expresión Génica , Interacciones Huésped-Patógeno/genética , Virus Vaccinia/fisiología , Vaccinia/genética , Vaccinia/virología , Animales , Línea Celular , Replicación del ADN , ADN Viral , Modelos Animales de Enfermedad , Proteína 1 de la Respuesta de Crecimiento Precoz/metabolismo , Fibroblastos/metabolismo , Fibroblastos/virología , Eliminación de Gen , Sistema de Señalización de MAP Quinasas , Ratones , Ratones Noqueados , Fosforilación , Vaccinia/metabolismo , Replicación ViralRESUMEN
BACKGROUND: Leptospirosis is caused by pathogenic spirochetes of the genus Leptospira spp. This zoonotic disease is distributed globally and affects domestic animals, including cattle. Leptospira interrogans serogroup Sejroe serovar Hardjo and Leptospira borgpetersenii serogroup Sejroe serovar Hardjo remain important species associated with this reproductive disease in livestock production. Previous studies on Brazilian livestock have reported that L. interrogans serovar Hardjo is the most prevalent leptospiral agent in this country and is related to clinical signs of leptospirosis, which lead to economic losses in production. Here, we described the isolation of three clinical strains (Norma, Lagoa and Bolivia) obtained from leptospirosis outbreaks that occurred in Minas Gerais state in 1994 and 2008. RESULTS: Serological and molecular typing using housekeeping (secY and 16SrRNA) and rfb locus (ORF22 and ORF36) genes were applied for the identification and comparative analysis of Leptospira spp. Our results identified the three isolates as L. interrogans serogroup Sejroe serovar Hardjo and confirmed the occurrence of this bacterial strain in Brazilian livestock. Genetic analysis using ORF22 and ORF36 grouped the Leptospira into serogroup Sejroe and subtype Hardjoprajitno. Genetic approaches were also applied to compare distinct serovars of L. interrogans strains by verifying the copy numbers of the IS1500 and IS1533 insertion sequences (ISs). The IS1500 copy number varied among the analyzed L. interrogans strains. CONCLUSION: This study provides evidence that L. interrogans serogroup Sejroe serovar Hardjo subtype Hardjoprajitno causes bovine leptospirosis in Brazilian production. The molecular results suggested that rfb locus (ORF22 and ORF36) could improve epidemiological studies by allowing the identification of Leptospira spp. at the serogroup level. Additionally, the IS1500 and IS1533 IS copy number analysis suggested distinct genomic features among closely related leptospiral strains.
Asunto(s)
Enfermedades de los Bovinos/microbiología , Brotes de Enfermedades/veterinaria , Leptospira interrogans/aislamiento & purificación , Leptospirosis/veterinaria , Animales , Brasil/epidemiología , Bovinos , Enfermedades de los Bovinos/epidemiología , Elementos Transponibles de ADN , ADN Bacteriano , ADN Ribosómico , Genes Bacterianos , Sitios Genéticos , Leptospira interrogans/clasificación , Leptospira interrogans/genética , Leptospirosis/epidemiología , Leptospirosis/microbiología , Tipificación Molecular , Sistemas de Lectura AbiertaRESUMEN
In this paper we show that the absorption spectrum of the microalgae Nannochloropsis oceanica exhibits changes in response to the modulation of incident light. A model was used to analyze the contribution of different active pigments to the total absorption in the photosynthetically active radiation region and suggested consistent diel oscillations in the optical activity of carotenoids.
RESUMEN
Sugarcane ethanol is produced at large scale generating wastes that could be used for microalgae biomass production in a biorefinery strategy. In this study, forty microalgae strains were screened for growth in sugarcane vinasse at different concentrations. Two microalgae strains, Micractinium sp. Embrapa|LBA32 and C. biconvexa Embrapa|LBA40, presented vigorous growth in a light-dependent manner even in undiluted vinasse under non-axenic conditions. Microalgae strains presented higher biomass productivity in vinasse-based media compared to standard Bold's Basal Medium in cultures performed using 15L airlift flat plate photobioreactors. Chemical composition analyses showed that proteins and carbohydrates comprise the major fractions of algal biomass. Glucose was the main monosaccharide detected, ranging from 46% to 76% of the total carbohydrates content according to the strain and culture media used. This research highlights the potential of using residues derived from ethanol plants to cultivate microalgae for the production of energy and bioproducts.
Asunto(s)
Técnicas de Cultivo de Célula/métodos , Microalgas/crecimiento & desarrollo , Saccharum/química , Residuos , Biomasa , Carbohidratos/análisis , Etanol/metabolismo , Microalgas/metabolismo , Fotobiorreactores/microbiologíaRESUMEN
This study evaluated the feasibility of using the Ribulose Bisphosphate Carboxylase Large subunit gene (rbcL) and the Internal Transcribed Spacers 1 and 2 of the nuclear rDNA (nuITS1 and nuITS2) markers for identifying a very diverse, albeit poorly known group, of green microalgae from neotropical inland waters. Fifty-one freshwater green microalgae strains isolated from Brazil, the largest biodiversity reservoir in the neotropics, were submitted to DNA barcoding. Currently available universal primers for ITS1-5.8S-ITS2 region amplification were sufficient to successfully amplify and sequence 47 (92%) of the samples. On the other hand, new sets of primers had to be designed for rbcL, which allowed 96% of the samples to be sequenced. Thirty-five percent of the strains could be unambiguously identified to the species level based either on nuITS1 or nuITS2 sequences' using barcode gap calculations. nuITS2 Compensatory Base Change (CBC) and ITS1-5.8S-ITS2 region phylogenetic analysis, together with morphological inspection, confirmed the identification accuracy. In contrast, only 6% of the strains could be assigned to the correct species based solely on rbcL sequences. In conclusion, the data presented here indicates that either nuITS1 or nuITS2 are useful markers for DNA barcoding of freshwater green microalgae, with advantage for nuITS2 due to the larger availability of analytical tools and reference barcodes deposited at databases for this marker.
Asunto(s)
Chlorophyta/clasificación , Chlorophyta/genética , Código de Barras del ADN Taxonómico , Microalgas/clasificación , Microalgas/genética , Brasil , ADN de Plantas , ADN Espaciador Ribosómico , Marcadores Genéticos , Filogenia , Análisis de Secuencia de ADNRESUMEN
Resumo O objetivo do presente estudo foi analisar o equilíbrio dinâmico em praticantes experientes e iniciantes de Brazilian Jiu-Jitsu (BJJ) e grupo controle. A amostra do presente estudo foi composta por 34 participantes, com idades entre 20 e 42 anos, divididos em três grupos: 10 praticantes de BJJ Experientes, 12 praticantes de BJJ Iniciantes e 12 não praticantes de BJJ que compuseram o grupo controle. A tarefa do protocolo consistiu na busca do equilíbrio sobre um estabilômetro, com o objetivo de manter a plataforma o mais perto possível da posição horizontal (ângulo de 0 a 3º de desvio máximo) durante cada tentativa de 90 segundos. Os resultados demonstram diferenças significativas entre os grupos [F(2,31) = 30,24; p < 0,001]. Através dos testes de "post-hoc" foram detectadas diferenças entre o grupo Experientes (46 ± 14 s) e os outros dois grupos, Iniciantes (25 ± 6 s, p < 0,001) e Grupo Controle (19 ± 4 s, p < 0,001). Todavia, não foram encontradas diferenças significativas entre os Grupo Controle e Iniciantes (p = 0,421). Os resultados sugerem que o tempo de prática de BJJ pode influenciar no desempenho do controle do equilíbrio.
Abstract The aim of the present study was to analyze the dynamic balance in experienced and beginners practitioners of Brazilian Jiu-Jitsu (BJJ) and control group. The sample was composed by 34 participants with ages ranging between 20 and 42 years old, divided into three groups: 10 experienced BJJ practitioners, 12 beginners BJJ practitioners and 12 non-practitioners of BJJ that composed the control group. The task of the protocol consisted on the search for balance over a stabilometer, aiming at keeping the platform as close as possible of the horizontal position during each attempt. The results show significant differences among the groups [F(2.31) = 30,24; p < 0.001]. The post-hoc test revealed differences among the experienced BJJ group (46 ± 14 s) and the other groups (Beginners BJJ: 25 ± 6 s, p < 0.001; Control group: 19 ± 4 s, p < 0.001). However, no significant differences were found between control and beginners BJJ groups (p = 0.421). The results suggest that the time of BJJ practice may influence on the balance control performance.
Asunto(s)
Humanos , Adulto , Desempeño Psicomotor , Artes Marciales , Destreza Motora , Recolección de DatosRESUMEN
Medicinal plants are used throughout the world, and the regulations defining their proper use, such as identification of the correct species and verification of the presence, purity and concentration of the required chemical compounds, are widely recognized. Herbal medicines are made from vegetal drugs, the processed products of medicinal species. These processed materials present a number of challenges in terms of botanical identification, and according to the World Health Organization (WHO), the use of incorrect species is a threat to consumer safety. The samples used in this study consisted of the dried leaves, flowers and roots of 257 samples from 8 distinct species approved by the WHO for the production of medicinal herbs and sold in Brazilian markets. Identification of the samples in this study using DNA barcoding (matK, rbcL and ITS2 regions) revealed that the level of substitutions may be as high as 71%. Using qualitative and quantitative chemical analyses, this study identified situations in which the correct species was being sold, but the chemical compounds were not present. Even more troubling, some samples identified as substitutions using DNA barcoding contained the chemical compounds from the correct species at the minimum required concentration. This last situation may lead to the use of unknown species or species whose safety for human consumption remains unknown. This study concludes that DNA barcoding should be used in a complementary manner for species identification with chemical analyses to detect and quantify the required chemical compounds, thus improving the quality of this class of medicines.
Asunto(s)
ADN de Plantas/genética , Plantas Medicinales/genética , Brasil , Código de Barras del ADN Taxonómico/métodos , Flores/genética , Humanos , Hojas de la Planta/genética , Raíces de Plantas/genética , Análisis de Secuencia de ADN/métodos , Organización Mundial de la SaludRESUMEN
The virus responsible for an outbreak of infectious laryngotracheitis (ILT) in a multi-age flock of egg layer chickens under quarantine in Brazil was characterized. Layer chickens from this area with circulating gallid herpesvirus 1 (GaHV 1) were evaluated using histopathology and molecular characterization techniques based on sequences of infected-cell polypeptide 4 (ICP4) and thymidine kinase (TK) genes. The infected chickens that were analyzed were PCR-positive for GaHV-1 in the trachea and negative in most trigeminal ganglia. The lack of ILT lesions in the conjunctiva and respiratory tissues, combined with detection of viral DNA in the trachea, was found to be associated with latent infection. The sequences from five farms obtained in the present study were identical, and there were no deletions within the 272- to 283-bp region of the ICP4 gene, as observed in the sequences of vaccine strains (CEO and TCO). The lack of a deletion in the ICP4 fragment analyzed in this study indicates that the chickens were infected with a field virus. The absence of the T252M mutation in a fragment of the TK gene, in addition to the low mortality rate observed, suggests that the outbreak in the state of Minas Gerais was not caused by a highly virulent strain but rather by a field virus of lower virulence. In addition, using phylogenetic reconstructions, it was found that this field strain was grouped together in a separate branch, apart from the previously characterized Brazilian strains. The introduction of vectored vaccines apparently has been effective in reducing clinical disease and lesions, and preventing new outbreaks of disease.
Asunto(s)
Pollos , Infecciones por Herpesviridae/veterinaria , Herpesvirus Gallináceo 1/aislamiento & purificación , Oviposición/fisiología , Enfermedades de las Aves de Corral/virología , Animales , Secuencia de Bases , Brasil/epidemiología , ADN Viral/genética , Femenino , Regulación Viral de la Expresión Génica/fisiología , Infecciones por Herpesviridae/epidemiología , Infecciones por Herpesviridae/virología , Herpesvirus Gallináceo 1/genética , Filogenia , Enfermedades de las Aves de Corral/epidemiología , Proteínas Virales/genética , Proteínas Virales/metabolismoRESUMEN
The purpose of the present study was to analyze the electromyographic (EMG) signals of the rectus femoris (RF), vastus lateralis (VL), semitendinosus (ST) and short head of the biceps femoris (BF) during the performance of stationary running at different intensities in aquatic and dry land environments. The sample consisted of 12 female volunteers who performed the stationary running exercise in aquatic and dry land environments at a submaximal cadence (80 beats·min(-1) controlled by a metronome) and at maximal velocity, with EMG signal measurements from the RF, VL, ST and BF muscles. The results showed a distinct pattern between environments for each muscle examined. For the submaximal cadence of 80 beats·min(-1), there was a reduced magnitude of the EMG signal in the aquatic environment, except for the ST muscle, the pattern of which was similar in both environments. In contrast to the submaximal cadence, the pattern of the EMG signal from all of the muscles showed similar magnitudes for both environments and phases of movement at maximal velocity, except for the VL muscle. Therefore, the EMG signals from the RF, VL, ST and BF muscles of women during stationary running had different patterns of activation over the range of motion between aquatic and dry land environments for different intensities. Moreover, the neuromuscular responses of the lower limbs were optimized by an increase in intensity from submaximal cadence to maximal velocity.
RESUMEN
Helminthiases are among the most important livestock diseases worldwide, in particular for small ruminants, which are the focus of this review. Resource Allocation Theory implies that high-productivity farm animals proportionate insufficient resources for adequate coping with stressful conditions. Significant differences between breeds and within breeds are seen, as well as genotype vs. environment interactions. With improvement of genetic host resistance to infection, transmission of infection will be impacted. On the other hand, genetic improvement of resilience can lead to a reduction in clinical signs of disease, but not necessarily reduce transmission of infection to other animals. Faecal egg count (FEC) is the main measurement used to evaluate helminthiasis load, despite the fact that the protocols and analytical methods can affect the results, and the FEC data frequently shows aggregative, negative skewed distribution, and a high coefficient of variation. Mass selection where heritability is generally medium to low generally produces slow results and low economic returns. Many studies have been published linking resistance to nematodes in livestock to Quantitative Trait Loci and most studies have concentrated on chromosomes where the major histocompatibility complex region is located. Nevertheless, these complex traits have been seen to be affected by thousands of variants that each has a small effect. More recent studies have shown that genome-wide selection strategies can be useful in selecting animals for improved production and resistance traits in this case.
Asunto(s)
Crianza de Animales Domésticos/métodos , Cruzamiento/métodos , Helmintiasis Animal/prevención & control , Selección Genética , Enfermedades de las Ovejas/prevención & control , Alelos , Animales , Antihelmínticos/uso terapéutico , Biomarcadores , Resistencia a la Enfermedad/genética , Resistencia a Medicamentos/genética , Evolución Molecular , Interacción Gen-Ambiente , Estudios de Asociación Genética/veterinaria , Genética de Población , Estudio de Asociación del Genoma Completo/veterinaria , Enfermedades de las Cabras/tratamiento farmacológico , Enfermedades de las Cabras/genética , Enfermedades de las Cabras/prevención & control , Cabras/genética , Cabras/parasitología , Helmintiasis Animal/tratamiento farmacológico , Helmintiasis Animal/genética , Interacciones Huésped-Parásitos/genética , Humanos , Mutación , Recuento de Huevos de Parásitos , Sitios de Carácter Cuantitativo , Ovinos/genética , Ovinos/parasitología , Enfermedades de las Ovejas/tratamiento farmacológico , Enfermedades de las Ovejas/genética , Especificidad de la EspecieRESUMEN
ETHNOPHARMACOLOGICAL RELEVANCE: Quina is a popular name originally attributed to Cinchona pubescens Vahl (=Cinchona succirubra) and Cinchona. calisaya Wedd., species native from Peru that have the antimalarial alkaloid quinine. In Brazil, bitter barks substitutes for the Peruvian species began to be used centuries ago, and they still are sold in popular markets. To assess the authenticity and the conditions on which samples of quinas have been commercialized, using the DNA barcode, chemical and biological assays. MATERIALS AND METHODS: Starting with 28 samples of barks acquired on a popular market, 23 had their DNA extracted successfully. The regions matK and rbcL were amplified and sequenced for 15 and 23 samples, respectively. Phytochemical analyses were performed by chromatographic methods, and biological essays were done by antimalarial tests in vitro. RESULTS: The identified species belonged to six different families, many of them endangered or with no correlation with use in traditional medicine as a Brazilian quina. The absence of typical bitter chemical substances indicated that barks have been collected from other species or from very young trees. The results of biological essays confirm the lack of standardization of the sold materials. CONCLUSION: The integrated approaches proved to be efficient to evaluate medicinal plants sold in popular markets and can be useful for promoting their better use and conservation.
Asunto(s)
Cinchona/química , Conservación de los Recursos Naturales , Medicina Tradicional/métodos , Plantas Medicinales/química , Antimaláricos/química , Antimaláricos/economía , Antimaláricos/aislamiento & purificación , Secuencia de Bases , Brasil , Cinchona/genética , Comercio , Código de Barras del ADN Taxonómico , Etnofarmacología , Humanos , Medicina Tradicional/economía , Corteza de la Planta , Extractos Vegetales/química , Extractos Vegetales/economía , Extractos Vegetales/farmacología , Plantas Medicinales/genéticaRESUMEN
Anthelmintic resistance is an increasing problem that threatens livestock production worldwide. Understanding of the genetic basis of benzimidazole resistance recently allowed the development of promising molecular diagnostic tools. In this study, isolates of Haemonchus contortus obtained from goats, sheep and buffaloes raised in Brazil were screened for presence of the polymorphism Phe200Tyr in the β-tubulin 1 gene, which confers resistance to benzimidazole. The allelic frequency of the mutation conferring resistance ranged from 7% to 43%, and indicated that resistance to benzimidazole could be found in nematodes isolated from all the ruminant species surveyed. Although significant variation in the frequency of the F200Y mutation was observed between different herds or host species, no significant variation could be found in populations isolated from animals within the same herd. These findings suggest that screening of samples from a few animals has the potential to provide information about the benzimidazole resistance status of the entire herd, which would enable a considerable reduction in the costs of diagnosis for the producer. Molecular diagnosis has practical advantages, since it can guide the choice of anthelmintic drug that will be used, before its application in the herd, thus reducing the economic losses driven by anthelmintic resistance.
A resistência aos anti-helmínticos é um problema crescente que ameaça a produção pecuária em todo o mundo. A compreensão da base genética da resistência ao benzimidazol permitiu, recentemente, o desenvolvimento de métodos diagnósticos moleculares promissores. Neste estudo, isolados de Haemonchus contortus obtidos a partir de rebanhos de caprinos, ovinos e bubalinos criados no Brasil foram avaliados quanto à presença do polimorfismo F200Y no gene da β-tubulina1, o qual confere resistência ao benzimidazol. A frequência alélica da mutação variou de 7% a 43%, indicando que a resistência ao benzimidazol pode ser encontrada em nematoides isolados a partir de todas as espécies de ruminantes pesquisadas. Embora tenha sido observada variação significativa das frequências de mutação F200Y entre rebanhos/espécies hospedeiros distintos, não foi encontrada variação significativa entre populações isoladas de animais dentro de um mesmo rebanho. Estes achados sugerem que a avaliação de amostras de alguns poucos animais tem o potencial de fornecer informações sobre o nível de resistência ao benzimidazol de todo o rebanho, possibilitando uma redução considerável dos custos de diagnóstico para o produtor. O diagnóstico molecular apresenta vantagens práticas, uma vez que pode guiar a escolha da base anti-helmíntica a ser utilizada antes da sua aplicação no rebanho, reduzindo, portanto, as perdas ocasionadas pela resistência aos fármacos anti-helmínticos.
Asunto(s)
Animales , Femenino , Masculino , Antihelmínticos/farmacología , Antihelmínticos/uso terapéutico , Bencimidazoles/farmacología , Bencimidazoles/uso terapéutico , Búfalos/parasitología , Enfermedades de las Cabras/tratamiento farmacológico , Cabras/parasitología , Hemoncosis/veterinaria , Haemonchus/efectos de los fármacos , Enfermedades de las Ovejas/tratamiento farmacológico , Ovinos/parasitología , Resistencia a Medicamentos/genética , Enfermedades de las Cabras/parasitología , Hemoncosis/tratamiento farmacológico , Hemoncosis/parasitología , Haemonchus/genética , Mutación , Enfermedades de las Ovejas/parasitologíaRESUMEN
A recent (November 2010) outbreak of infectious laryngotracheitis (ILT) in a multi-age laying hen facility in Minas Gerais state, Brazil, is described. Previous ILT outbreak in laying hens was only notified in São Paulo state, Brazil, in 2002. In the outbreak described here, the affected population was approximately eight million hens, with flock sizes ranging from 100,000 to 2,900,000 chickens. The average mortality ranged from 1 to 6%, and morbidity was around 90% (most of the twenty seven farms of the area were positive for ILT virus). Three multi-age laying farms from one company were selected for this report. Clinical signs included prostration, dyspnea, conjunctivitis, occasional swelling of the paranasal sinuses and bloody mucous nasal discharge. Severely affected chickens presented with dyspnea, gasping and became cyanotic before death. At necropsy, these chickens had fibrinous exudate blocking the larynx and the lumen of cranial part of the trachea. In addition, conjunctivitis with intense hyperemia, edema and sinuses with caseous exudate were present. On histopathology, there were marked necrosis and desquamation of respiratory ephitelium and conjunctiva with numerous syncytial cells formation and fibrinous exudate. Moderate to marked non suppurative (especially lymphocytes and plasma cells) infiltration in the lamina propria also was observed. Sixteen out of 20 examined chickens, eosinophilic intranuclear inclusion bodies were observed in the syncytial cells. The DNA extracted from larynx and trachea produced positive PCR results for ILT virus (ILTV) DNA using formalin-fixed, paraffin embedded (FFPE) samples. Amplicons from a small region of ICP4 gene were submitted to sequencing and showed 100% identity with ILTV EU104910.1 (USA strain), 99% with ILTV JN596963.1 (Australian strain) and 91% with ILTV JN580316.1 (Gallid herpesvirus 1 CEO vaccine strain) and JN580315.1 (Gallid herpesvirus 1 TCO vaccine strain).
Um surto recente (Novembro de 2010) de laringotraqueite infecciosa (LTI) em granjas de postura de múltiplas idades em Minas Gerais, Brasil, é descrito. Um surto de LTI em galinhas de postura havia sido previamente relatado apenas no Estado de São Paulo em 2002. No surto aqui descrito, a população afetada foi de aproximadamente oito milhões de galinhas, com lotes variando de 100.000 a 2.900.000 galinhas. A mortalidade média variou de 1 a 6% e a morbidade atingiu cerca de 90% (a maioria das 27 granjas foram positivas para o virus da LTI). Três granjas com aves de múltiplas idades pertencentes a uma empresa foram selecionadas para o presente relato. Os sinais clinicos incluíram prostração, dispneia, conjuntivite, edema ocasional dos seios paranasais e secreção nasal mucosa e/ou sanguinolenta. As aves severamente afetadas apresentaram acentuada dispneia, aparente engasgo e tornaram-se cianóticas antes da morte. Nestas aves, exsudato fibrinoso denso obstruindo o lúmen da laringe e parte cranial da traqueia foi observado na necropsia. Havia também, conjuntivite com hiperemia intensa e edema, além de sinusite com exsudato caseoso. Na histopatologia, observaram-se necrose e descamação acentuada do epitélio respiratório e da conjuntiva com formação de numerosos sincícios e exsudato fibrinoso. Além disso, infiltrado inflamatório mononuclear (especialmente linfócitos e plasmócitos) moderado a acentuado na lâmina própria foi observado. Corpúsculos de inclusão intranucleares nas células sinciciais foram observados em 16 das 20 aves examinadas. Resultados positivos pela PCR para o virus da LTI foram obtidos de DNA extraído das laringes e traqueias utilizando amostras fixadas em formol e incluidas na parafina. O produto amplificado de uma região pequena do gen ICP4 foi submetido ao sequenciamento e quando comparado com outras sequências depositadas no Genbank mostrou os seguintes resultados: 100% de identidade com uma estirpe do virus de LTI dos Estados Unidos (JN596963.1), 99% de identidade com uma estirpe Australiana e 91% com a estirpe vacinal CEO (JN580316.1) e TCO (JN580315.1).
Asunto(s)
Animales , Pollos/virología , Herpesvirus Gallináceo 1/aislamiento & purificación , Reacción en Cadena de la Polimerasa/veterinaria , Disnea/veterinariaRESUMEN
A recent (November 2010) outbreak of infectious laryngotracheitis (ILT) in a multi-age laying hen facility in Minas Gerais state, Brazil, is described. Previous ILT outbreak in laying hens was only notified in São Paulo state, Brazil, in 2002. In the outbreak described here, the affected population was approximately eight million hens, with flock sizes ranging from 100,000 to 2,900,000 chickens. The average mortality ranged from 1 to 6%, and morbidity was around 90% (most of the twenty seven farms of the area were positive for ILT virus). Three multi-age laying farms from one company were selected for this report. Clinical signs included prostration, dyspnea, conjunctivitis, occasional swelling of the paranasal sinuses and bloody mucous nasal discharge. Severely affected chickens presented with dyspnea, gasping and became cyanotic before death. At necropsy, these chickens had fibrinous exudate blocking the larynx and the lumen of cranial part of the trachea. In addition, conjunctivitis with intense hyperemia, edema and sinuses with caseous exudate were present. On histopathology, there were marked necrosis and desquamation of respiratory ephitelium and conjunctiva with numerous syncytial cells formation and fibrinous exudate. Moderate to marked non suppurative (especially lymphocytes and plasma cells) infiltration in the lamina propria also was observed. Sixteen out of 20 examined chickens, eosinophilic intranuclear inclusion bodies were observed in the syncytial cells. The DNA extracted from larynx and trachea produced positive PCR results for ILT virus (ILTV) DNA using formalin-fixed, paraffin embedded (FFPE) samples. Amplicons from a small region of ICP4 gene were submitted to sequencing and showed 100% identity with ILTV EU104910.1 (USA strain), 99% with ILTV JN596963.1 (Australian strain) and 91% with ILTV JN580316.1 (Gallid herpesvirus 1 CEO vaccine strain) and JN580315.1 (Gallid herpesvirus 1 TCO vaccine strain).(AU)
Um surto recente (Novembro de 2010) de laringotraqueite infecciosa (LTI) em granjas de postura de múltiplas idades em Minas Gerais, Brasil, é descrito. Um surto de LTI em galinhas de postura havia sido previamente relatado apenas no Estado de São Paulo em 2002. No surto aqui descrito, a população afetada foi de aproximadamente oito milhões de galinhas, com lotes variando de 100.000 a 2.900.000 galinhas. A mortalidade média variou de 1 a 6% e a morbidade atingiu cerca de 90% (a maioria das 27 granjas foram positivas para o virus da LTI). Três granjas com aves de múltiplas idades pertencentes a uma empresa foram selecionadas para o presente relato. Os sinais clinicos incluíram prostração, dispneia, conjuntivite, edema ocasional dos seios paranasais e secreção nasal mucosa e/ou sanguinolenta. As aves severamente afetadas apresentaram acentuada dispneia, aparente engasgo e tornaram-se cianóticas antes da morte. Nestas aves, exsudato fibrinoso denso obstruindo o lúmen da laringe e parte cranial da traqueia foi observado na necropsia. Havia também, conjuntivite com hiperemia intensa e edema, além de sinusite com exsudato caseoso. Na histopatologia, observaram-se necrose e descamação acentuada do epitélio respiratório e da conjuntiva com formação de numerosos sincícios e exsudato fibrinoso. Além disso, infiltrado inflamatório mononuclear (especialmente linfócitos e plasmócitos) moderado a acentuado na lâmina própria foi observado. Corpúsculos de inclusão intranucleares nas células sinciciais foram observados em 16 das 20 aves examinadas. Resultados positivos pela PCR para o virus da LTI foram obtidos de DNA extraído das laringes e traqueias utilizando amostras fixadas em formol e incluidas na parafina. O produto amplificado de uma região pequena do gen ICP4 foi submetido ao sequenciamento e quando comparado com outras sequências depositadas no Genbank mostrou os seguintes resultados: 100% de identidade com uma estirpe do virus de LTI dos Estados Unidos (JN596963.1), 99% de identidade com uma estirpe Australiana e 91% com a estirpe vacinal CEO (JN580316.1) e TCO (JN580315.1).(AU)