RESUMEN
The aims of this flow cytometry study were to quantify B lymphoid precursors known as hématogones across age and clinical conditions and to study the immunophenotypic profile of these benign immature B cells. A total of 406 consecutive marrow specimens were analyzed for hématogones using 4-color flow cytometry during a 19 month period (60% males and 40% females). The age range was 3 months to 89 years. Hématogones were present in 80% of the specimens. Morphologic analysis of the smears from each patient showed small numbers of hématogones (<13% of total cellularity). The B cell population was defined by CD19 + CD45 bright positivity, coexpression of other B lineage markers: CD20, CD22, CD10, CD29, CD38 and CD58 in addition to HLA-DR and CD34. In our study we found a significant decline in hématogones with increasing age but a broad range was found at all ages. Marrow from some adults contained relatively high numbers. Diagnosis in these patients included cytopenias, infections, and neoplastic diseases. Distinction of hématogones is critical for disease management particularly after therapy of paediatric B acute lymphoblastic leukaemia to monitor for minimal residual disease.
Asunto(s)
Células Precursoras de Linfocitos B/citología , Células Precursoras de Linfocitos B/inmunología , Adolescente , Adulto , Anciano , Anciano de 80 o más Años , Envejecimiento/inmunología , Envejecimiento/patología , Antígenos CD/metabolismo , Niño , Preescolar , Femenino , Citometría de Flujo , Humanos , Inmunofenotipificación , Lactante , Masculino , Persona de Mediana Edad , Estudios Prospectivos , Adulto JovenRESUMEN
The rat biliary and urinary metabolism of N2,N6-dimethyl-9-hydroxyellipticinium (an N6-methyl derivative of elliptinium acetate, an antitumor agent) is reported. Two main metabolites have been identified: the glucuronide and sulfate derivatives by conjugation of the hydroxy group at position 9. Excretion profiles in bile and urine are also given. It has to be noted that no metabolite corresponding to a demethylation at the indolic nitrogen has been identified. However, the evidence for an increased concentration of the oxidized form of glutathione in bile during the drug excretion supports the hypothesis of an oxidative metabolism of this drug in rat liver.
Asunto(s)
Alcaloides/farmacocinética , Antineoplásicos/farmacocinética , Bilis/metabolismo , Elipticinas/farmacocinética , Animales , Antineoplásicos/orina , Biotransformación , Fenómenos Químicos , Química Física , Cromatografía Líquida de Alta Presión , Elipticinas/orina , Glutatión/metabolismo , Masculino , Ratas , Ratas Endogámicas , Factores de TiempoRESUMEN
Evidences for the formation of the glutathione-hydroxyellipticine adduct during the rat metabolism of 9-methoxyellipticine are provided. These data suggest that such a cytotoxic molecule might behave as a pro-alkylating agent in vivo.