RESUMEN

A new immunocytochemical methodology has been applied to value the proliferation in the periodontal tissue following experimental tooth movement. Orthodontic tooth movement has been obtained in Guinea pigs by a coil sporing, and a proliferation marker administrated "in vivo"; the proliferation marker used is Bromodeoxyuridine, a thimidine analogue which is taken up by DNA synthesizing nuclei, and afterwards intensified by two monoclonal antibodies, where the second is FITC coniugated. Instead of some technical difficulties, the results are encouraging for further development.

Asunto(s)

Resorción Ósea/patología , Periodoncio/patología , Técnicas de Movimiento Dental/efectos adversos , Animales , Anticuerpos Monoclonales , Resorción Ósea/etiología , Resorción Ósea/inmunología , Bromodesoxiuridina , División Celular , Cobayas , Inmunohistoquímica , Aparatos Ortodóncicos/efectos adversos