RESUMEN

One of the most economically important bacterial pathogens of plants and plant products is Dickeya dadantii. This bacterium causes soft rot disease in tubers and other parts of the potato and other plants of the Solanaceae family. The application of restricted host range bacteriophages as biocontrol agents has recently gained widespread interest. This study purposed to isolate the infectious agent of the potato and evaluate its biocontrol by bacteriophages. Two phytopathogenic strains were isolated from infected potatoes, identified based on biochemical and 16S rRNA gene sequencing, and submitted to GenBank as D. dadantii strain pis3 (accession no. HQ423668) and D. dadantii strain sip4 (accession no. HQ423669). Their bacteriophages were isolated from Caspian Sea water by enriching the water filtrate with D. dadantii strains as hosts using spot or overlay methods. On the basis of morphotypes, the isolated bacteriophages were identified as members of the Myoviridae and Siphoviridae families and could inhibit the growth of antibiotic resistant D. dadantii strains in culture medium. Moreover, in Dickeya infected plants treated with bacteriophage, no disease progression was detected. No significant difference was seen between phage-treated and control plants. Thus, isolated bacteriophages can be suggested for the biocontrol of plant disease caused by Dickeya strains.

Asunto(s)

Bacteriófagos/aislamiento & purificación , Agentes de Control Biológico/aislamiento & purificación , Dickeya chrysanthemi/crecimiento & desarrollo , Dickeya chrysanthemi/virología , Enfermedades de las Plantas/microbiología , Solanum tuberosum/microbiología , Bacteriófagos/clasificación , Secuencia de Bases , Agentes de Control Biológico/clasificación , ADN Bacteriano/genética , Dickeya chrysanthemi/efectos de los fármacos , Dickeya chrysanthemi/aislamiento & purificación , Pruebas de Sensibilidad Microbiana , Datos de Secuencia Molecular , Myoviridae/clasificación , Myoviridae/aislamiento & purificación , ARN Ribosómico 16S/genética , Análisis de Secuencia de ADN , Siphoviridae/clasificación , Siphoviridae/aislamiento & purificación

RESUMEN

One of the most economically important bacterial pathogens of plants and plant products is Dickeya dadantii. This bacterium causes soft rot disease in tubers and other parts of the potato and other plants of the Solanaceae family. The application of restricted host range bacteriophages as biocontrol agents has recently gained widespread interest. This study purposed to isolate the infectious agent of the potato and evaluate its biocontrol by bacteriophages. Two phytopathogenic strains were isolated from infected potatoes, identified based on biochemical and 16S rRNA gene sequencing, and submitted to GenBank as D. dadantii strain pis3 (accession no. HQ423668) and D. dadantii strain sip4 (accession no. HQ423669). Their bacteriophages were isolated from Caspian Sea water by enriching the water filtrate with D. dadantii strains as hosts using spot or overlay methods. On the basis of morphotypes, the isolated bacteriophages were identified as members of the Myoviridae and Siphoviridae families and could inhibit the growth of antibiotic resistant D. dadantii strains in culture medium. Moreover, in Dickeya infected plants treated with bacteriophage, no disease progression was detected. No significant difference was seen between phage-treated and control plants. Thus, isolated bacteriophages can be suggested for the biocontrol of plant disease caused by Dickeya strains..(AU)

Asunto(s)

Bacteriófagos/aislamiento & purificación , Agentes de Control Biológico/aislamiento & purificación , Dickeya chrysanthemi/crecimiento & desarrollo , Dickeya chrysanthemi/virología , Enfermedades de las Plantas/microbiología , Bacteriófagos/clasificación , Secuencia de Bases , Agentes de Control Biológico/clasificación , ADN Bacteriano/genética , Pruebas de Sensibilidad Microbiana , Datos de Secuencia Molecular , Myoviridae/clasificación , Myoviridae/aislamiento & purificación , Dickeya chrysanthemi , Dickeya chrysanthemi/aislamiento & purificación , /genética , Análisis de Secuencia de ADN , Siphoviridae/clasificación , Siphoviridae/aislamiento & purificación

RESUMEN

One of the most economically important bacterial pathogens of plants and plant products is Dickeya dadantii. This bacterium causes soft rot disease in tubers and other parts of the potato and other plants of the Solanaceae family. The application of restricted host range bacteriophages as biocontrol agents has recently gained widespread interest. This study purposed to isolate the infectious agent of the potato and evaluate its biocontrol by bacteriophages. Two phytopathogenic strains were isolated from infected potatoes, identified based on biochemical and 16S rRNA gene sequencing, and submitted to GenBank as D. dadantii strain pis3 (accession no. HQ423668) and D. dadantii strain sip4 (accession no. HQ423669). Their bacteriophages were isolated from Caspian Sea water by enriching the water filtrate with D. dadantii strains as hosts using spot or overlay methods. On the basis of morphotypes, the isolated bacteriophages were identified as members of the Myoviridae and Siphoviridae families and could inhibit the growth of antibiotic resistant D. dadantii strains in culture medium. Moreover, in Dickeya infected plants treated with bacteriophage, no disease progression was detected. No significant difference was seen between phage-treated and control plants. Thus, isolated bacteriophages can be suggested for the biocontrol of plant disease caused by Dickeya strains.

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Asunto(s)

Bacteriófagos/aislamiento & purificación , Agentes de Control Biológico/aislamiento & purificación , Dickeya chrysanthemi/crecimiento & desarrollo , Dickeya chrysanthemi/virología , Enfermedades de las Plantas/microbiología , Solanum tuberosum/microbiología , Secuencia de Bases , Bacteriófagos/clasificación , Agentes de Control Biológico/clasificación , ADN Bacteriano/genética , Pruebas de Sensibilidad Microbiana , Datos de Secuencia Molecular , Myoviridae/clasificación , Myoviridae/aislamiento & purificación , Dickeya chrysanthemi/efectos de los fármacos , Dickeya chrysanthemi/aislamiento & purificación , /genética , Análisis de Secuencia de ADN , Siphoviridae/clasificación , Siphoviridae/aislamiento & purificación