RESUMEN
UNLABELLED: The diversity of the cultivable microbiota of the marine sponge Phorbas tenacior frequently found in the Mediterranean Sea was investigated, and its potential as a source of antimicrobial, antioxidant and antiplasmodial compounds was evaluated. The cultivable bacterial community was studied by isolation, cultivation and 16S rRNA gene sequencing. Twenty-three bacterial strains were isolated and identified in the Proteobacteria (α or γ classes) and Actinobacteria phyla. Furthermore, three different bacterial morphotypes localized extracellularly within the sponge tissues were revealed by microscopic observations. Bacterial strains were assigned to seven different genera, namely Vibrio, Photobacterium, Shewanella, Pseudomonas, Ruegeria, Pseudovibrio and Citricoccus. The strains affiliated to the same genus were differentiated according to their genetic dissimilarities using random amplified polymorphic DNA (RAPD) analyses. Eleven bacterial strains were selected for evaluation of their bioactivities. Three isolates Pseudovibrio P1Ma4, Vibrio P1MaNal1 and Citricoccus P1S7 revealed antimicrobial activity; Citricoccus P1S7 and Vibrio P1MaNal1 isolates also exhibited antiplasmodial activity, while two Vibrio isolates P1Ma8 and P1Ma5 displayed antioxidant activity. These data confirmed the importance of Proteobacteria and Actinobacteria associated with marine sponges as a reservoir of bioactive compounds. SIGNIFICANCE AND IMPACT OF THE STUDY: This study presents the first report on the diversity of the cultivable bacteria associated with the marine sponge Phorbas tenacior, frequently found in the Mediterranean Sea. Evaluation of the antiplasmodial, antimicrobial and antioxidant activities of the isolates has been investigated and allowed to select bacterial strains, confirming the importance of Proteobacteria and Actinobacteria as sources of bioactive compounds.
Asunto(s)
Actinobacteria/aislamiento & purificación , Microbiota , Poríferos/microbiología , Proteobacteria/aislamiento & purificación , Actinobacteria/clasificación , Actinobacteria/genética , Actinobacteria/fisiología , Animales , Antibiosis , Biodiversidad , Genes de ARNr , Mar Mediterráneo , Micrococcaceae/clasificación , Micrococcaceae/genética , Micrococcaceae/aislamiento & purificación , Micrococcaceae/fisiología , Filogenia , Plasmodium falciparum/fisiología , Proteobacteria/clasificación , Proteobacteria/genética , Proteobacteria/fisiología , ARN Ribosómico 16S/genética , Técnica del ADN Polimorfo Amplificado Aleatorio , Rhodobacteraceae/clasificación , Rhodobacteraceae/genética , Rhodobacteraceae/aislamiento & purificación , Rhodobacteraceae/fisiología , Vibrio/clasificación , Vibrio/genética , Vibrio/aislamiento & purificación , Vibrio/fisiologíaRESUMEN
Several molecules isolated from various marine organisms (microorganisms, algae, fungi, invertebrates, and vertebrates) are currently under study at an advanced stage of clinical trials, either directly or in the form of analogues deduced from structure-activity relationships. Some of them have already been marketed as drugs. The goal of this article is not to present a complete panorama of marine pharmacology but to show that new models and new mechanisms of action of marine substances bring new solutions for tackling some of the major public health problems of the 21st century. These include: malaria, which assails mainly the southern hemisphere; tuberculosis, an infectious disease once believed to be eliminated but alarmingly increasing, especially among HIV-positive populations; and osteoporosis and Alzheimer's disease, the extension of which are correlated with ageing populations, especially in the developed countries.
Asunto(s)
Enfermedad de Alzheimer/tratamiento farmacológico , Productos Biológicos/uso terapéutico , Enfermedades Transmisibles/tratamiento farmacológico , Industria Farmacéutica/métodos , Biología Marina/métodos , Osteoporosis/tratamiento farmacológico , Farmacología/métodos , Acuicultura/métodos , Acuicultura/tendencias , Ingeniería Biomédica/métodos , Ingeniería Biomédica/tendencias , Biotecnología/métodos , Biotecnología/tendencias , Industria Farmacéutica/tendencias , Humanos , Biología Marina/tendencias , Farmacología/tendenciasRESUMEN
The alkaloid harman, previously reported from some marine invertebrates, was identified as the antibiotic substance of the tunicate-associated bacterium Enterococcus faecium. It exhibited antibacterial activity (MIC, 0.017 mM) against the ichthyopathogenic strain Vibrio anguillarum.
Asunto(s)
Antibacterianos/aislamiento & purificación , Enterococcus faecium/metabolismo , Harmina/análogos & derivados , Harmina/aislamiento & purificación , Urocordados/microbiología , Animales , Antibacterianos/biosíntesis , Antibacterianos/farmacología , Enterococcus faecium/aislamiento & purificación , Harmina/biosíntesis , Harmina/farmacología , Pruebas de Sensibilidad Microbiana , Staphylococcus aureus/efectos de los fármacos , Vibrio/efectos de los fármacosRESUMEN
A methanol adduct of puupehenone (1), 15alpha-methoxypuupehenol (2), an artifact resulting from the action of MeOH on puupehenone, was isolated during purification of the CH(2)Cl(2) extract of the New Caledonian marine sponge Hyrtios sp., as the major constituent. Its chemical structure was elucidated by 2D NMR experiments. Compound 2 displayed similar antimicrobial and antifungal activity as puupehenone and a lower cytotoxic activity toward KB cells with ED(50) values of 6 and 0.5 microg/mL, respectively. Compound 2 was slightly more active against three strains of Plasmodium falciparum than puupehenone.
Asunto(s)
Poríferos/química , Sesquiterpenos/aislamiento & purificación , Animales , Decápodos/efectos de los fármacos , Ensayos de Selección de Medicamentos Antitumorales , Humanos , Metanol/química , Pruebas de Sensibilidad Microbiana , Plasmodium falciparum/efectos de los fármacos , Sesquiterpenos/química , Sesquiterpenos/farmacología , Análisis EspectralRESUMEN
We found that the magnesium salt of ilimaquinone, named 201-F, specifically disassembled dynamically unstable microtubules in fibroblasts and various epithelial cell lines. Unlike classical tubulin- interacting drugs such as nocodazole or colchicine which affect all classes of microtubules, 201-F did not depolymerize stable microtubules. In WIF-B-polarized hepatic cells, 201-F disrupted the Golgi complex and inhibited albumin and alpha1-antitrypsin secretion to the same extent as nocodazole. By contrast, 201-F did not impair the transport of membrane proteins to the basolateral surface, which was only affected by the total disassembly of cellular microtubules. Transcytosis of two apical membrane proteins-the alkaline phosphodiesterase B10 and dipeptidyl peptidase IV-was affected to the same extent by 201-F and nocodazole. Taken together, these results indicate that only dynamically unstable microtubules are involved in the transport of secretory proteins to the plasma membrane, and in the transcytosis of membrane proteins to the apical surface. By contrast, stable microtubules, which are not functionally affected by 201-F treatment, are involved in the transport of membrane proteins to the basolateral surface. By specifically disassembling highly dynamic microtubules, 201-F is an invaluable tool with which to study the functional specialization of stable and dynamic microtubules in living cells.
Asunto(s)
Microtúbulos/metabolismo , Proteínas/metabolismo , Albúminas/metabolismo , Animales , Transporte Biológico , Línea Celular , Perros , Células HeLa , Humanos , Microtúbulos/efectos de los fármacos , Nocodazol/farmacología , Fosfodiesterasa I , Hidrolasas Diéster Fosfóricas/metabolismo , Quinonas/farmacología , alfa 1-Antitripsina/metabolismoRESUMEN
The influence of sesquiterpene quinones and of a sesquiterpene hydroquinone, isolated from the sponge Smenospongia sp. on normal and tumour cells, was investigated. Most showed cytotoxic effects on L1210 leukemia cells. However, their activity on normal cells, such as murine spleen lymphocytes and human peripheral lymphocytes, revealed different behaviours: some of them inhibited, while other enhanced mitogen-stimulated lymphocyte proliferation. These biological studies revealed products modulating immune responses.