RESUMEN

Three Brucella abortus strains were isolated from joint hygromas from cows in northern Togo. Two deletions in the 5' side of the gene BruAb2_0168 were identified. As this gene is used for species identification, these deletions have consequences for diagnostic procedures. Multiple locus variable number of tandem repeat (VNTR) analysis was therefore performed for species identification. The strains showed unique VNTR profiles, providing some of the first genotypic data from West Africa. More molecular and epidemiological data are needed from the region, in order to better understand transmission patterns and develop suitable diagnostic assays.

Asunto(s)

Brucella abortus/genética , Brucella abortus/aislamiento & purificación , Enfermedades de los Bovinos/diagnóstico , Genes Bacterianos , Linfangioma Quístico/veterinaria , Técnicas de Diagnóstico Molecular/métodos , Eliminación de Secuencia , Animales , Bovinos , Enfermedades de los Bovinos/microbiología , ADN Bacteriano/química , ADN Bacteriano/genética , Errores Diagnósticos , Linfangioma Quístico/diagnóstico , Datos de Secuencia Molecular , Análisis de Secuencia de ADN , Togo