RESUMEN
The present study aimed to evaluate the in vitro antibacterial and antioxidant activities and the in vivo wound healing performance of a polysaccharide isolated from Glycyrrhiza glabra named PSG. It was structurally characterized by Fourier transformed infrared (FT-IR) spectroscopy, which confirmed the presence of different polysaccharides functional bands. The antioxidant capacity of PSG was determined in vitro and evaluated in vivo through the examination of wound healing capacity. Thirty two rats were randomly divided into four groups: group I was treated with physiological serum (negative control); group II was treated with "CYTOL CENTELLA®"; group III was treated with glycerol and group IV was treated with polysaccharide. The response to treatments was assessed by macroscopic, histologic, and biochemical parameters. Data revealed that our sample exhibited potential antioxidant activities and accelerated significantly the wound healing process, after ten days of treatment, proved by the higher wound appearance scores and a higher content of collagen confirmed by histological examination, when compared with control and "CYTOL CENTELLA®". Overall, these findings proved that this polysaccharide isolated from Glycyrrhiza glabra could be considered as a natural bioactive polymer for therapeutic process in wound healing applications.
O presente estudo teve como objetivo avaliar as atividades antibacteriana e antioxidante in vitro e o desempenho cicatricial in vivo de um polissacarídeo isolado de Glycyrrhiza glabra denominado PSG. Foi caracterizado estruturalmente por espectroscopia no infravermelho com transformada de Fourier (FT-IR), que confirmou a presença de diferentes bandas funcionais de polissacarídeos. A capacidade antioxidante da PSG foi determinada in vitro e avaliada in vivo através do exame da capacidade de cicatrização de feridas. Trinta e dois ratos foram divididos aleatoriamente em quatro grupos: o grupo I foi tratado com soro fisiológico (controle negativo); o grupo II foi tratado com "CYTOL CENTELLA®"; o grupo III foi tratado com glicerol e o grupo IV foi tratado com polissacarídeo. A resposta aos tratamentos foi avaliada por parâmetros macroscópicos, histológicos e bioquímicos. Os dados revelaram que nossa amostra apresentou atividades antioxidantes potenciais e acelerou significativamente o processo de cicatrização da ferida, após dez dias de tratamento, comprovado pelos maiores escores de aparência da ferida e maior teor de colágeno confirmado pelo exame histológico, quando comparado ao controle e "CYTOL CENTELLA® ". No geral, esses achados provaram que esse polissacarídeo isolado de Glycyrrhiza glabra pode ser considerado um polímero bioativo natural para processos terapêuticos em aplicações de cicatrização de feridas.
Asunto(s)
Animales , Ratas , Polisacáridos/efectos adversos , Cicatrización de Heridas , Ratas Wistar , Glycyrrhiza , AntioxidantesRESUMEN
The present study aimed to evaluate the in vitro antibacterial and antioxidant activities and the in vivo wound healing performance of a polysaccharide isolated from Glycyrrhiza glabra named PSG. It was structurally characterized by Fourier transformed infrared (FT-IR) spectroscopy, which confirmed the presence of different polysaccharides functional bands. The antioxidant capacity of PSG was determined in vitro and evaluated in vivo through the examination of wound healing capacity. Thirty two rats were randomly divided into four groups: group I was treated with physiological serum (negative control); group II was treated with "CYTOL CENTELLA®"; group III was treated with glycerol and group IV was treated with polysaccharide. The response to treatments was assessed by macroscopic, histologic, and biochemical parameters. Data revealed that our sample exhibited potential antioxidant activities and accelerated significantly the wound healing process, after ten days of treatment, proved by the higher wound appearance scores and a higher content of collagen confirmed by histological examination, when compared with control and "CYTOL CENTELLA®". Overall, these findings proved that this polysaccharide isolated from Glycyrrhiza glabra could be considered as a natural bioactive polymer for therapeutic process in wound healing applications.