RESUMEN
Cardiovascular disease is the number one cause of death in the United States. Deployment of stents and vascular grafts has been a major therapeutic method for treatment. However, restenosis, incomplete endothelialization, and thrombosis hamper the long term clinical success. As a solution to meet these current challenges, we have developed a native endothelial ECM mimicking self-assembled nanofibrous matrix to serve as a new treatment model. The nanofibrous matrix is formed by self-assembly of peptide amphiphiles (PAs), which contain nitric oxide (NO) donating residues, endothelial cell adhesive ligands composed of YIGSR peptide sequence, and enzyme-mediated degradable sites. NO was successfully released from the nanofibrous matrix rapidly within 48 h, followed by sustained release over period of 30 days. The NO releasing nanofibrous matrix demonstrated a significantly enhanced proliferation of endothelial cells (51+/-3% to 67+/-2%) but reduced proliferation of smooth muscle cells (35+/-2% to 16+/-3%) after 48 h of incubation. There was also a 150-fold decrease in platelet attachment on the NO releasing nanofibrous matrix (470+/-220 platelets/cm(2)) compared to the collagen-I (73+/-22 x 10(3)platelets/cm(2)) coated surface. The nanofibrous matrix has the potential to be applied to various cardiovascular implants as a self-assembled coating, thereby providing a native endothelial extracellular matrix (ECM) mimicking environment.
Asunto(s)
Materiales Biomiméticos/farmacología , Materiales Biocompatibles Revestidos/farmacología , Endotelio/efectos de los fármacos , Óxido Nítrico/metabolismo , Péptidos/farmacología , Tensoactivos/farmacología , Secuencia de Aminoácidos , Aorta/citología , Prótesis Vascular , Implantación de Prótesis Vascular , Adhesión Celular/efectos de los fármacos , Proliferación Celular , Colágeno Tipo I/farmacología , Células Endoteliales/citología , Células Endoteliales/efectos de los fármacos , Humanos , Concentración de Iones de Hidrógeno/efectos de los fármacos , Microscopía Fluorescente , Datos de Secuencia Molecular , Miocitos del Músculo Liso/citología , Miocitos del Músculo Liso/efectos de los fármacos , Nanofibras/ultraestructura , Péptidos/química , Adhesividad Plaquetaria/efectos de los fármacos , Solventes , Acero Inoxidable/farmacología , Venas Umbilicales/citologíaRESUMEN
Nitrosothiols (RSNO), formed from thiols and metabolites of nitric oxide (*NO), have been implicated in a diverse set of physiological and pathophysiological processes, although the exact mechanisms by which they are formed biologically are unknown. Several candidate nitrosative pathways involve the reaction of *NO with O(2), reactive oxygen species (ROS), and transition metals. We developed a strategy using extracellular ferrocyanide to determine that under our conditions intracellular protein RSNO formation occurs from reaction of *NO inside the cell, as opposed to cellular entry of nitrosative reactants from the extracellular compartment. Using this method we found that in RAW 264.7 cells RSNO formation occurs only at very low (<8 microM) O(2) concentrations and exhibits zero-order dependence on *NO concentration. Indeed, RSNO formation is not inhibited even at O(2) levels <1 microM. Additionally, chelation of intracellular chelatable iron pool (CIP) reduces RSNO formation by >50%. One possible metal-dependent, O(2)-independent nitrosative pathway is the reaction of thiols with dinitrosyliron complexes (DNIC), which are formed in cells from the reaction of *NO with the CIP. Under our conditions, DNIC formation, like RSNO formation, is inhibited by approximately 50% after chelation of labile iron. Both DNIC and RSNO are also increased during overproduction of ROS by the redox cycler 5,8-dimethoxy-1,4-naphthoquinone. Taken together, these data strongly suggest that cellular RSNO are formed from free *NO via transnitrosation from DNIC derived from the CIP. We have examined in detail the kinetics and mechanism of RSNO formation inside cells.
Asunto(s)
Hierro/metabolismo , Macrófagos/metabolismo , Óxido Nítrico/metabolismo , Óxidos de Nitrógeno/metabolismo , Proteínas/metabolismo , S-Nitrosotioles/metabolismo , Animales , Hipoxia de la Célula , Línea Celular , Espacio Extracelular/metabolismo , Espacio Intracelular/metabolismo , Quelantes del Hierro/metabolismo , Macrófagos/citología , Ratones , Oxidación-Reducción , Estrés Oxidativo , Oxígeno , Especies Reactivas de Oxígeno/metabolismo , Factores de TiempoRESUMEN
Respiratory syncytial virus (RSV) infection has been shown to reduce Na+-driven alveolar fluid clearance in BALB/c mice in vivo. To investigate the cellular mechanisms by which RSV inhibits amiloride-sensitive epithelial Na+ channels (ENaC), the main pathways through which Na+ ions enter lung epithelial cells, we infected human Clara-like lung (H441) cells with RSV that expresses green fluorescent protein (rRA2). 3-6 days later patch clamp recordings showed that infected cells (i.e. cells expressing green fluorescence; GFP+) had significantly lower whole-cell amiloride-sensitive currents and single channel activity (NPo) as compared with non-infected (GFP-), non-inoculated, or cells infected with UV-inactivated RSV. Both alpha and beta ENaC mRNA levels were significantly reduced in GFP+ cells as measured by real-time reverse transcription-PCR. Infection with RSV increased expression of the inducible nitric-oxide synthase (iNOS) and nitrite concentration in the culture medium; nuclear translocation of NF-kappaB p65 subunit and NF-kappaB activation were also up-regulated. iNOS up-regulation in GFP+ cells was prevented by knocking down IkappaB kinase gamma before infection. Furthermore, pretreatment of H441 cells with the specific iNOS inhibitor 1400W (1 microM) resulted in a doubling of the amiloride-sensitive Na+ current in GFP+ cells. Additionally, preincubation of H441 cells with A77-1726 (20 microM), a de novo UTP synthesis inhibitor, and 1400W completely reversed the RSV inhibition of amiloride-sensitive currents in GFP+ cells. Thus, both UTP- and iNOS-generated reactive species contribute to ENaC down-regulation in RSV-infected airway epithelial cells.
Asunto(s)
Células Epiteliales/metabolismo , Canales Epiteliales de Sodio/biosíntesis , Óxido Nítrico Sintasa de Tipo II/metabolismo , Mucosa Respiratoria/metabolismo , Infecciones por Virus Sincitial Respiratorio/metabolismo , Virus Sincitiales Respiratorios/metabolismo , Uridina Trifosfato/metabolismo , Compuestos de Anilina/farmacología , Animales , Antiinflamatorios no Esteroideos/farmacología , Línea Celular , Crotonatos , Regulación hacia Abajo/efectos de los fármacos , Regulación hacia Abajo/genética , Células Epiteliales/virología , Canales Epiteliales de Sodio/genética , Técnicas de Silenciamiento del Gen , Humanos , Hidroxibutiratos/farmacología , Quinasa I-kappa B/genética , Quinasa I-kappa B/metabolismo , Pulmón , Ratones , Ratones Endogámicos BALB C , Óxido Nítrico Sintasa de Tipo II/genética , Nitrilos , Nitritos/metabolismo , Mucosa Respiratoria/virología , Infecciones por Virus Sincitial Respiratorio/genética , Virus Sincitiales Respiratorios/genética , Sodio , Toluidinas , Factor de Transcripción ReIA/genética , Factor de Transcripción ReIA/metabolismo , Regulación hacia Arriba/efectos de los fármacos , Regulación hacia Arriba/genética , Uridina Trifosfato/genéticaRESUMEN
Recent advances in techniques that allow sensitive and specific measurement of S-nitrosothiols (RSNOs) have provided evidence for a role for these compounds in various aspects of nitric oxide (NO) biology. The most widely used approach is to couple reaction chemistry that selectively reduces RSNOs by one electron to produce NO, with the sensitive detection of the latter under anaerobic conditions using ozone based chemiluminescence in NO analyzers. Herein, we report a novel reaction that is readily adaptable for commercial NO analyzers that utilizes hydrogen sulfide (H2S), a gas that can reduce RSNO to NO and, analogous to NO, is produced by endogenous metabolism and has effects on diverse biological functions. We discuss factors that affect H2S based methods for RSNO measurement and discuss the potential of H2S as an experimental tool to measure RSNO.
Asunto(s)
Sulfuro de Hidrógeno , S-Nitrosotioles/análisis , Animales , Humanos , S-Nitrosotioles/químicaRESUMEN
One of the most important biological reactions of nitric oxide (nitrogen monoxide, *NO) is its reaction with transition metals, of which iron is the major target. This is confirmed by the ubiquitous formation of EPR-detectable g=2.04 signals in cells, tissues, and animals upon exposure to both exogenous and endogenous *NO. The source of the iron for these dinitrosyliron complexes (DNIC), and its relationship to cellular iron homeostasis, is not clear. Evidence has shown that the chelatable iron pool (CIP) may be at least partially responsible for this iron, but quantitation and kinetic characterization have not been reported. In the murine cell line RAW 264.7, *NO reacts with the CIP similarly to the strong chelator salicylaldehyde isonicotinoyl hydrazone (SIH) in rapidly releasing iron from the iron-calcein complex. SIH pretreatment prevents DNIC formation from *NO, and SIH added during the *NO treatment "freezes" DNIC levels, showing that the complexes are formed from the CIP, and they are stable (resistant to SIH). DNIC formation requires free *NO, because addition of oxyhemoglobin prevents formation from either *NO donor or S-nitrosocysteine, the latter treatment resulting in 100-fold higher intracellular nitrosothiol levels. EPR measurement of the CIP using desferroxamine shows quantitative conversion of CIP into DNIC by *NO. In conclusion, the CIP is rapidly and quantitatively converted to paramagnetic large molecular mass DNIC from exposure to free *NO but not from cellular nitrosothiol. These results have important implications for the antioxidative actions of *NO and its effects on cellular iron homeostasis.
Asunto(s)
Hierro/química , Óxido Nítrico/metabolismo , Animales , Antioxidantes/química , Línea Celular , Quelantes/química , Quelantes/farmacología , Espectroscopía de Resonancia por Spin del Electrón , Hemoglobinas/química , Homeostasis , Sustancias Macromoleculares , Ratones , Microscopía Fluorescente/métodos , Modelos Químicos , Nitrógeno/química , Compuestos de Sulfhidrilo/químicaRESUMEN
We investigated the cellular mechanisms by which nitric oxide (NO) increases chloride (Cl-) secretion across lung epithelial cells in vitro and in vivo. Addition of (Z)-1-[2-(2-aminoethyl)-N-(2-ammonioethyl) amino] diazen-1-ium-1, 2-diolate (DETANONOate [DETANO];1-1,000 microM) into apical compartments of Ussing chambers containing Calu-3 cells increased short-circuit currents (I(sc)) from 5.2 +/- 0.8 to 15.0 +/- 2.1 microA/cm(2) (X +/- 1 SE; n = 7; P < 0.001). NO generated from two nitrated lipids (nitrolinoleic and nitrooleic acids; 1-10 microM) also increased I(sc) by about 100%. Similar effects were noted across basolaterally, but not apically, permeabilized Calu-3 cells. None of these NO donors increased I(sc) in Calu-3 cells pretreated with 10 microM 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one (an inhibitor of soluble guanylyl cyclase). Scavenging of NO either prevented or reversed the increase of I(sc). These data indicate that NO stimulation of soluble guanylyl cyclase was sufficient and necessary for the increase of I(sc) via stimulation of the apical cystic fibrosis transmembrane regulator (CFTR). Both Calu-3 and alveolar type II (ATII) cells contained CFTR, as demonstrated by in vitro phosphorylation of immunoprecipitated CFTR by protein kinase (PK) A. PKGII (but not PKGI) phosphorylated CFTR immuniprecipitated from Calu-3 cells. Corresponding values in ATII cells were below the threshold of detection. Furthermore, DETANO, 8-Br-cGMP, or 8-(4-chlorophenylthio)-cGMP (up to 2 mM each) did not increase Cl- secretion across amiloride-treated ATII cells in vitro. Measurements of nasal potential differences in anesthetized mice showed that perfusion of the nares with DETANO activated glybenclamide-sensitive Cl- secretion. These findings suggest that small concentrations of NO donors may prove beneficial in stimulating Cl- secretion across airway cells without promoting alveolar edema.
Asunto(s)
Cloruros/metabolismo , Ácidos Linoleicos/farmacología , Pulmón/metabolismo , Nitrocompuestos/farmacología , Compuestos Nitrosos/farmacología , Ácidos Oléicos/farmacología , Animales , Línea Celular , Polaridad Celular , GMP Cíclico/análogos & derivados , GMP Cíclico/farmacología , Regulador de Conductancia de Transmembrana de Fibrosis Quística/metabolismo , Células Epiteliales/efectos de los fármacos , Células Epiteliales/metabolismo , Guanilato Ciclasa/antagonistas & inhibidores , Guanilato Ciclasa/metabolismo , Transporte Iónico , Pulmón/citología , Pulmón/efectos de los fármacos , Ratones , Óxido Nítrico/fisiología , Donantes de Óxido Nítrico/farmacología , Especies de Nitrógeno Reactivo/metabolismo , Especies de Nitrógeno Reactivo/farmacología , Receptores Citoplasmáticos y Nucleares/antagonistas & inhibidores , Receptores Citoplasmáticos y Nucleares/metabolismo , Mucosa Respiratoria/citología , Mucosa Respiratoria/efectos de los fármacos , Mucosa Respiratoria/metabolismo , Guanilil Ciclasa Soluble , Tionucleótidos/farmacologíaRESUMEN
We investigated the mechanisms by which S-nitrosoglutathione (GSNO) alters cystic fibrosis transmembrane conductance regulator (CFTR) mediated chloride (Cl(-)) secretion across Calu-3 cells, an extensively used model of human airway gland serous cells. Confluent monolayers of Calu-3 cells, grown under an air-liquid interface, were mounted in Ussing chambers for the measurements of chloride short circuit current (I(sc)) and trans-epithelial resistance (R(t)). Addition of GSNO into the apical compartment of these chambers resulted in significant and sustained increase of I(sc) with an IC(50) of 3.2 +/- 1 mum (mean +/- 1 S.E.; n = 6). Addition of either glibenclamide or pre-treatment of Calu-3 cells with the soluble guanylate cyclase inhibitor 1H-(1,2,4)-oxadiazolo[4,3-a]quinoxalin-1-one totally prevented the GSNO-induced increase of I(sc). Conversely, BAY 41-2272, a sGC stimulator, increased I(sc) in a dose-response fashion. The GSNO increase of I(sc) was reversed by addition of two phosphatases (PP2A1, PP2A2) into the apical compartment of Ussing chambers containing Calu-3 monolayers. Oxy-myoglobin (oxy-Mb, 300 mum) added into the apical compartment of Ussing chambers either prior or after GSNO either completely prevented or immediately reversed the increase of I(sc). However, smaller concentrations of oxy-Mb (1-10 mum), sufficient to scavenge NO in the medium (as assessed by direct measurement of NO in the Ussing chamber using an ISO-NO meter) decreased I(sc) partially. Oxy-Mb did not reverse the increase of I(sc) following addition of GSNO and cysteine (50 mum). These findings indicate that GSNO stimulates Cl secretion via both cGMP-dependent and cGMP-independent mechanisms.
Asunto(s)
Broncodilatadores/farmacología , Cloruros/farmacocinética , Regulador de Conductancia de Transmembrana de Fibrosis Quística/efectos de los fármacos , Regulador de Conductancia de Transmembrana de Fibrosis Quística/fisiología , Óxido Nítrico/metabolismo , S-Nitrosoglutatión/farmacología , Técnicas de Cultivo de Célula , Canales de Cloruro/fisiología , GMP Cíclico/metabolismo , Regulador de Conductancia de Transmembrana de Fibrosis Quística/metabolismo , Electrofisiología , Humanos , Chaperonas Moleculares , Óxido Nítrico/análisis , Fosforilación , Especies de Nitrógeno Reactivo , Mucosa Respiratoria/citologíaRESUMEN
RATIONALE: Mycoplasma pneumoniae is a significant cause of pneumonia in humans. OBJECTIVES: To determine the impact of mycoplasma infection and the host inflammatory response on alveolar type II (ATII) cell ion transport in vivo and in vitro. METHODS: Mice were infected with M. pulmonis for measurements of alveolar fluid clearance (AFC) in vivo and isolation of ATII cells. ATII cells were infected in vivo for determination of epithelial Na+ channel (ENaC) total and cell surface protein levels by biotinylation and Western blot and in vitro for whole cell patch clamp recording and measurement of nitric oxide (NO) production by chemiluminescence. RESULTS: Mycoplasma infection significantly inhibited AFC at 24 h and total and amiloride-sensitive AFC by 48 h postinfection (pi). In contrast, infected myeloperoxidase-deficient mice had similar basal and amiloride-sensitive AFC values to uninfected control mice at 48 h pi. Addition of forskolin restored total and amiloride-sensitive AFC to control values at 48 h pi. ATII cells isolated from infected mice demonstrated normal alpha, beta, and gamma ENaC total protein levels; however, infected whole-lung cell-surface levels of gamma ENaC were significantly decreased. Patch-clamp recordings demonstrated a significant decrease in total and amiloride-sensitive Na+ currents at 24 h pi. ATII cells demonstrated a significant increase in the production of NO at 24 h pi and inhibition of NO by ATII cells before infection reversed the decrease in total Na+ currents. CONCLUSIONS: These data indicate that mycoplasma infection results in decreased AFC and functional ENaC via the production of reactive oxygen nitrogen intermediates.
Asunto(s)
Infecciones por Mycoplasma/metabolismo , Mycoplasma pulmonis , Neumonía Bacteriana/metabolismo , Alveolos Pulmonares/metabolismo , Mucosa Respiratoria/metabolismo , Canales de Sodio/metabolismo , Animales , Técnicas de Cultivo de Célula , Ratones , Neumonía Bacteriana/microbiología , Especies de Nitrógeno Reactivo/fisiología , Especies Reactivas de OxígenoRESUMEN
Patterns of protein expression were examined in white skeletal muscle from adult zebrafish (Danio rerio). High resolution two-dimensional gel electrophoresis resolved between 300 and 400 spots with molecular masses between 20 and 120 kDa and isoelectric points between about 5 and 8. Forty spots, representing a range of protein size, charge, and abundance were excised, digested with trypsin, and subjected to matrix-assisted laser-desorption/ionisation-time of flight mass spectrometry for protein identification. Twenty-nine spots were identified, including enzymes of energy metabolism, contractile proteins, an iron transport protein, and a heat shock protein. In addition, several spots matched theoretical proteins predicted from genome sequencing. These theoretical proteins were tentatively identified by similarity to known proteins. Patterns of muscle protein expression were then measured after zebrafish were exposed to low oxygen (16 torr) for 48 h, an exposure previously shown to increase the survival of zebrafish at more severe reductions in oxygen. Exposure to low oxygen (hypoxia) did not change the general pattern of protein expression but did affect the amounts of six low abundance proteins. The relatively subtle effects of hypoxia on patterns of muscle protein expression contrasts the widespread changes previously documented in mRNA levels in this and other species of fish during hypoxic stress. The difference between protein and mRNA expression illustrates the need to integrate both measures for a more complete understanding of gene expression in fish during hypoxic exposure.