RESUMEN

An improved synthesis of the precursor acetic acid-piperidine-4-yl ester by acetylation of 4-hydroxypiperidine hydrochloride in anhydrous chloroform was developed. A procedure for fast evaluation and characterization of products originated by acetylation of the 4-piperidinol using LC-APCI/MS with an acetonitrile-water gradient method on a Merck Purosphere RP-18 column was also developed. The highly purified precursor allowed the production of [11C]MP4A for PET studies of acetylcholine neurotransmission system. The tracer was produced with >98% radiochemical purity, with yields ranging 20-60% (decay-corrected) from EOB.

Asunto(s)

Acetatos/síntesis química , Hidrocarburos Yodados/química , Piperidinas/síntesis química , Radiofármacos/síntesis química , Acetatos/química , Acetilación , Acetilcolinesterasa/análisis , Cromatografía Líquida de Alta Presión , Cromatografía de Gases y Espectrometría de Masas , Humanos , Marcaje Isotópico/métodos , Espectroscopía de Resonancia Magnética , Piperidinas/química , Tomografía de Emisión de Positrones/métodos , Radiofármacos/química

Asunto(s)

Meningitis/diagnóstico