RESUMEN
To correct defects of the body's phagocytic system, the authors used experimental agents, such as vital activity products of plant cells, which were obtained by biotechnology. These included arabinogalactan, syringin, K-212, and DFEGP. The study agents activate the oxidative metabolism of macrophages, the production of active forms of oxygen, and thus enhance its bactericidal effect against Yersinia pseudotuberculosis. Syringin and arabinogalactan produce a more pronounced stimulating macrophageal effect in Yersinia pseudotuberculosis phagocytosis than do K-212 and DFEGP. The findings indicate that further studies of the effects of these tested and other agents on humoral and cellular immunity are required.
Asunto(s)
Adyuvantes Inmunológicos/farmacología , Fagocitosis/efectos de los fármacos , Yersinia pseudotuberculosis/fisiología , Animales , Galactanos/farmacología , Glucósidos/farmacología , Cobayas , Técnicas In Vitro , Macrófagos Peritoneales/efectos de los fármacos , Macrófagos Peritoneales/inmunología , Macrófagos Peritoneales/microbiología , Fenilpropionatos/farmacología , Polisacáridos/farmacologíaRESUMEN
The study used the selected aplasmid strain of Y. Pseudotuberculosis 53 and I-716, which contains plasmids that have molecular weights of 48 and 82 mD, and I-727 that has only virulence plasmid pYV48. The study has indicated that the fact that Yersinias have pVM82 and pYV48 enhances their resistance to phagocytosis. The Y. pseudotuberculosis strains carrying these plasmids suppress the responsiveness of phagocytes, inhibit an "oxidative explosion", lower the activities of superoxide dismutase and myeloperoxidase, thus suppressing their bactericidal capacity.
Asunto(s)
Fagocitosis , Plásmidos , Yersinia pseudotuberculosis/genética , Yersinia pseudotuberculosis/inmunología , Animales , Cobayas , Técnicas In Vitro , Macrófagos Peritoneales/microbiología , Peso MolecularRESUMEN
Electron-microscopic study of the interaction of Y. pestis and peritoneal phagocytes obtained from BALB mice, both intact and treated with the toxic fraction of Y. pestis, has revealed that in the process of phagocytosis the toxin weakens the killing and degradation phases of these bacteria, but leaves the ingestion activity of phagocytes unchanged. The process of phagocytosis in mice exposed to toxin is accompanied by destructive changes of cells and redistribution of myeloperoxidase, a bactericidal enzyme, in polymorphonuclear leukocytes without any signs of bacterial degradation.