RESUMEN
OBJECTIVES: To study membrane proteins modifications in Senescent Red Blood Cells (SeRBC). DESIGN ANDMETHODS: SeRBC were obtained on Percoll gradients. Membrane proteins were analyzed by SDS-PAGE, band 3 by immunoblotting, and protein oxidation by measuring the carbonyl groups. RESULTS: Densitometric analysis showed no change in SeRBC while an increase in band 3 and its degradation products was found. An increase of protein oxidation level was found in SeRBC. CONCLUSIONS: These findings provide further experimental evidence about protein modifications occurring during the RBC lifespan.
Asunto(s)
Proteína 1 de Intercambio de Anión de Eritrocito/metabolismo , Envejecimiento Eritrocítico , Eritrocitos/química , Proteínas de la Membrana/metabolismo , Electroforesis en Gel de Poliacrilamida , Eritrocitos/metabolismo , Humanos , Immunoblotting , Oxidación-Reducción , Procesamiento Proteico-PostraduccionalRESUMEN
The Duffy (FY) blood group system is clinically significant in transfusion medicine because FY antibodies are involved in hemolytic transfusion reactions and hemolytic disease of the newborn. The Fy(a) and Fy(b) antigens are encoded by the FY*A and FY*B alleles which are responsible for the Fy(a+b+), Fy(a+b-) and Fy(a-b+) phenotypes. The Fy(a-b-) phenotype is found in individuals homozygous for a silent FY*B allele, named FY*B ( ES ), which is caused by a mutation in the promoter region of FY*B that result in the loss of FY expression in the erythroid linage. The aim of the present study was to evaluate the role of FY DNA typing as a tool in transfusion compatibility testing. We studied 275 white blood donors from the city of Rosario by serological method and allele specific PCRs. We found that the 106 serologically Fy(a+b+) samples all genotyped as FY*A/FY*B (100%). Among the 94 Fy(a+b-) samples, 81 (86.2%) were FY*A/FY*A and 13 (13.8%) were FY*A/FY*B ( ES ) . Of the 75 Fy(a-b+) 67 (89.3%) were FY*B/FY*B and 8 (10.7%) were FY*B/FY*B ( ES ). No Fy(a-b-) samples were encountered. The frequencies of the FY*A, FY*B and FY*B ( ES ) alleles clearly revealed that the genetic pool analyzed is comprised of Caucasian and non-Caucasian alleles. These results showed that there is an important proportion of patients phenotyped as Fy(b-) that can be exposed to Fy(b+) blood units with no risk of alloimmunization when they carry the FY*A/FY*B ( ES ) genotype. Thus, FY genotyping allow increasing the pool of compatible units facilitating transfusion therapy and benefiting patients that require chronic transfusions.
Asunto(s)
Sistema del Grupo Sanguíneo Duffy/genética , Enfermedad Iatrogénica/prevención & control , Receptores de Superficie Celular/genética , Reacción a la Transfusión , Genotipo , Humanos , Reacción en Cadena de la Polimerasa , SerotipificaciónRESUMEN
BACKGROUND: Previous studies have suggested an influence of HLA molecules on the regulation of the anti Mycobacterium leprae immune response. METHODS: DNA typing of HLA-DRB1 alleles in 71 leprosy patients and 81 healthy controls was performed. Genomic DNA was extracted from peripheral blood and used as a template to amplify the polymorphic second exon of the HLA-DRB1 by the polymerase chain reaction (PCR). PCR products were hybridized separately with sequence-specific oligonucleotides. RESULTS: DRB1*1401 and DRB1*1406 alleles were significantly more prevalent in leprosy patients, whereas a decreased frequency of DRB1*0808 and DRB1*1103 alleles was found, by comparison with the group control. CONCLUSIONS: The HLA-DRB1 alleles could act alone or in combination with other genes to confer differential susceptibility and also protection to leprosy disease in endemic areas of the American continent.
Asunto(s)
Antígenos HLA-DR/genética , Lepra/epidemiología , Lepra/genética , Alelos , Argentina/epidemiología , Femenino , Cadenas HLA-DRB1 , Humanos , Masculino , Persona de Mediana EdadRESUMEN
We report the case of a pregnant woman sensitized with a panreactive anti-Rh17 alloantibody. Patient's red blood cells showed a partial deletion of Rh antigens, which was responsible for the alloimmunization. An autotransfusion program was instrumented so as to cover possible demands. Molecular analysis of the RH locus showed the presence of a hybrid RHCE-D(5-7)-CE allele that gave origin to the deleted phenotype.
Asunto(s)
Isoanticuerpos/inmunología , Complicaciones Hematológicas del Embarazo/inmunología , Isoinmunización Rh/inmunología , Sistema del Grupo Sanguíneo Rh-Hr/inmunología , Adulto , Femenino , Frecuencia de los Genes/inmunología , Genotipo , Humanos , Isoanticuerpos/sangre , Fenotipo , Embarazo , Complicaciones Hematológicas del Embarazo/sangre , Isoinmunización Rh/sangre , Sistema del Grupo Sanguíneo Rh-Hr/genéticaRESUMEN
Describimos el caso de una embarazada sensibilizada con un aloanticuerpo anti-Rh17 de muy amplia reactividad. Los glóbulos rojos de la paciente presentaban una deleción parcial de los antígenos del sistema Rh, responsable de la aloinmunización encontrada. Debido a la dificultad de obtener sangre compatible se elaboró un plan de transfusión autóloga para cubrir las posibles demandas. El análisis molecular del locus RH demostró la presencia de un alelo híbrido RHCE-D(5-7)-CE que generaba el fenotipo delecionado. (AU)
Asunto(s)
Humanos , Femenino , Embarazo , Adulto , Sistema del Grupo Sanguíneo Rh-Hr/inmunología , Isoinmunización Rh/sangre , Isoanticuerpos/inmunología , Complicaciones Hematológicas del Embarazo/sangre , Frecuencia de los Genes/inmunología , Fenotipo , GenotipoRESUMEN
Describimos el caso de una embarazada sensibilizada con un aloanticuerpo anti-Rh17 de muy amplia reactividad. Los glóbulos rojos de la paciente presentaban una deleción parcial de los antígenos del sistema Rh, responsable de la aloinmunización encontrada. Debido a la dificultad de obtener sangre compatible se elaboró un plan de transfusión autóloga para cubrir las posibles demandas. El análisis molecular del locus RH demostró la presencia de un alelo híbrido RHCE-D(5-7)-CE que generaba el fenotipo delecionado. (AU)
Asunto(s)
Humanos , Femenino , Embarazo , Adulto , Sistema del Grupo Sanguíneo Rh-Hr/inmunología , Isoinmunización Rh/sangre , Isoanticuerpos/inmunología , Complicaciones Hematológicas del Embarazo/sangre , Frecuencia de los Genes/inmunología , Fenotipo , GenotipoRESUMEN
Describimos el caso de una embarazada sensibilizada con un aloanticuerpo anti-Rh17 de muy amplia reactividad. Los glóbulos rojos de la paciente presentaban una deleción parcial de los antígenos del sistema Rh, responsable de la aloinmunización encontrada. Debido a la dificultad de obtener sangre compatible se elaboró un plan de transfusión autóloga para cubrir las posibles demandas. El análisis molecular del locus RH demostró la presencia de un alelo híbrido RHCE-D(5-7)-CE que generaba el fenotipo delecionado.