RESUMEN
We investigated the effect of fish oil (FO) supplementation on tumor growth, cyclooxygenase 2 (COX-2), peroxisome proliferator-activated receptor gamma (PPARγ), and RelA gene and protein expression in Walker 256 tumor-bearing rats. Male Wistar rats (70 days old) were fed with regular chow (group W) or chow supplemented with 1 g/kg body weight FO daily (group WFO) until they reached 100 days of age. Both groups were then inoculated with a suspension of Walker 256 ascitic tumor cells (3 × 10(7) cells/mL). After 14 days the rats were killed, total RNA was isolated from the tumor tissue, and relative mRNA expression was measured using the 2(-ΔΔCT) method. FO significantly decreased tumor growth (W=13.18 ± 1.58 vs WFO=5.40 ± 0.88 g, P<0.05). FO supplementation also resulted in a significant decrease in COX-2 (W=100.1 ± 1.62 vs WFO=59.39 ± 5.53, P<0.001) and PPARγ (W=100.4 ± 1.04 vs WFO=88.22 ± 1.46, P<0.05) protein expression. Relative mRNA expression was W=1.06 ± 0.022 vs WFO=0.31 ± 0.04 (P<0.001) for COX-2, W=1.08 ± 0.02 vs WFO=0.52 ± 0.08 (P<0.001) for PPARγ, and W=1.04 ± 0.02 vs WFO=0.82 ± 0.04 (P<0.05) for RelA. FO reduced tumor growth by attenuating inflammatory gene expression associated with carcinogenesis.
Asunto(s)
Carcinoma 256 de Walker/genética , Proliferación Celular/efectos de los fármacos , Ciclooxigenasa 2/genética , Aceites de Pescado/farmacología , PPAR gamma/genética , Factor de Transcripción ReIA/genética , Animales , Carcinoma 256 de Walker/metabolismo , Suplementos Dietéticos , Ácidos Docosahexaenoicos/farmacología , Ácido Eicosapentaenoico/farmacología , Aceites de Pescado/química , Inhibidores de Crecimiento/farmacología , Immunoblotting , Masculino , Ratas Wistar , Reacción en Cadena en Tiempo Real de la Polimerasa , Transcripción Genética/efectos de los fármacosRESUMEN
We investigated the effect of fish oil (FO) supplementation on tumor growth, cyclooxygenase 2 (COX-2), peroxisome proliferator-activated receptor gamma (PPARγ), and RelA gene and protein expression in Walker 256 tumor-bearing rats. Male Wistar rats (70 days old) were fed with regular chow (group W) or chow supplemented with 1 g/kg body weight FO daily (group WFO) until they reached 100 days of age. Both groups were then inoculated with a suspension of Walker 256 ascitic tumor cells (3×107 cells/mL). After 14 days the rats were killed, total RNA was isolated from the tumor tissue, and relative mRNA expression was measured using the 2-ΔΔCT method. FO significantly decreased tumor growth (W=13.18±1.58 vs WFO=5.40±0.88 g, P<0.05). FO supplementation also resulted in a significant decrease in COX-2 (W=100.1±1.62 vs WFO=59.39±5.53, P<0.001) and PPARγ (W=100.4±1.04 vs WFO=88.22±1.46, P<0.05) protein expression. Relative mRNA expression was W=1.06±0.022 vs WFO=0.31±0.04 (P<0.001) for COX-2, W=1.08±0.02 vs WFO=0.52±0.08 (P<0.001) for PPARγ, and W=1.04±0.02 vs WFO=0.82±0.04 (P<0.05) for RelA. FO reduced tumor growth by attenuating inflammatory gene expression associated with carcinogenesis.
Asunto(s)
Animales , Masculino , /genética , Proliferación Celular/efectos de los fármacos , /genética , Aceites de Pescado/farmacología , PPAR gamma/genética , Factor de Transcripción ReIA/genética , /metabolismo , Suplementos Dietéticos , Ácidos Docosahexaenoicos/farmacología , Ácido Eicosapentaenoico/farmacología , Aceites de Pescado/química , Inhibidores de Crecimiento/farmacología , Immunoblotting , Ratas Wistar , Reacción en Cadena en Tiempo Real de la Polimerasa , Transcripción Genética/efectos de los fármacosRESUMEN
The present work was designed to compare four commercial samples of quercetin, three of them presenting pharmaceutical grade (QPGa, QPGb and QPGc) and the other one pro-analysi grade (QPA) by means of different techniques. Physical and chromatographic characterization of these samples shows different properties following its origin, especially a clear evidence of polymorphism occurrence.