RESUMEN
This study exhibits the fabrication of target-specific Gold nanorods (GNRs) coupled with an anti-tumorigenic apoptotic drug and provides tracking of the labeled particles as they migrate through cells and release their drug-load to targeted cancer cells. We utilize the photoluminescence property of GNRs and their ability to be conjugated with multiple agents to transform facile rods to a targeted drug delivery vehicle. GNRs of aspect ratio 2.8 were conjugated with a targeting ligand, folic acid and an anthracycline drug, Doxorubicin. The multifunctional nanorods were then used to target folate receptor expressing cancers cells for the delivery of a concentration dependent dosage of Doxorubicin (DOX). By utilizing the photoluminescence of GNRs and the innate fluorescence of DOX, multi-photon fluorescence lifetime imaging was utilized to monitor the uptake of functionalized nanorods, the release of the drug and its localization in living cells. We show that these nano-vehicles successfully targeted cancer cells over expressing folate receptors and showed low toxicity to control cell lines. Release of DOX was observed in the cytoplasmic region and after 16 h was found to be redistributed in the nucleus resulting in cell death. Our theragnostic approach demonstrates the fabrication of multifunctional GNRs for targeted drug delivery and monitoring of the drug and the vehicle by multi-photon microscopy using fluorescence intensity and lifetime imaging.
Asunto(s)
Antibióticos Antineoplásicos/química , Doxorrubicina/análogos & derivados , Ácido Fólico/análogos & derivados , Oro/farmacología , Nanotubos/química , Antibióticos Antineoplásicos/síntesis química , Antibióticos Antineoplásicos/farmacología , Supervivencia Celular/efectos de los fármacos , Doxorrubicina/química , Doxorrubicina/farmacología , Sistemas de Liberación de Medicamentos/métodos , Ácido Fólico/química , Ácido Fólico/farmacología , Humanos , Células KB , Microscopía Electrónica de Transmisión , Microscopía Fluorescente , Nanotubos/ultraestructura , Espectrofotometría UltravioletaRESUMEN
Gold nanoprobes have become attractive diagnostic and therapeutic agents in medicine and life sciences research owing to their reproducible synthesis with atomic level precision, unique physical and chemical properties, versatility of their morphologies, flexibility in functionalization, ease of targeting, efficiency in drug delivery and opportunities for multimodal therapy. This review highlights some of the recent advances and the potential for gold nanoprobes in theranostics.
Asunto(s)
Portadores de Fármacos/química , Sistemas de Liberación de Medicamentos/métodos , Oro/química , Nanopartículas del Metal/química , Nanotecnología/métodos , Preparaciones Farmacéuticas/administración & dosificación , Técnicas Biosensibles/métodos , Portadores de Fármacos/uso terapéutico , Oro/uso terapéutico , Humanos , Nanopartículas del Metal/uso terapéutico , Preparaciones Farmacéuticas/química , Fármacos Fotosensibilizantes/química , Propiedades de SuperficieRESUMEN
We demonstrate a facile, one-step, protein-directed approach to preparing a new functional reduced graphene oxide via simultaneous reduction and surface functionalization of graphene oxide sheets with herceptin and show its potential application as a novel biological imaging agent.