RESUMEN
The influence of extremely high temperatures on the course of virus infection in vitro was studied. It has been shown that exposure of human cells (continuous cell line of embryonal skin-muscle tissue, donor blood leukocytes) to 43 degrees C leads to a significant increase in their sensitivity to cytopathic action of the viruses tested. Noteworthy, the reproduction of these viruses under tested conditions did not differ from their reproduction in control cells. A dramatic inhibitory effect of high temperatures on the interferon-producing capacity of cells was observed.
Asunto(s)
Virus de la Encefalomiocarditis/crecimiento & desarrollo , Virus de la Influenza A/crecimiento & desarrollo , Interferones/biosíntesis , Virus de la Enfermedad de Newcastle/inmunología , Virus de la Estomatitis Vesicular Indiana/crecimiento & desarrollo , Línea Celular , Efecto Citopatogénico Viral , Calor , HumanosRESUMEN
Pretreatment with crude interferon preparations obtained from suspension cultures of bone marrow, spleen and thymus cells or from mouse L-cell cultures or with mouse serum interferon preparations did not change the colony-forming activity of bone marrow cells on syngeneic transplantation to lethally irradiated mice. Preparations of L-cell culture interferon, dialysed and purified by carboxymethyl-Sephadex (G-25) column chromatography, showed an inhibitory effect on exogenous colony formation by bone marrow cells. The results suggested the presence in crude interferon preparations of a substance either inhibiting the anticellular effect of interferon or stimulating colony formation. The factor produced by thymus cells following their treatment with antilymphocyte serum inhibited colony formation by bone marrow cells and, unlike interferon, possessed no antiviral activity when tested in cell cultures.