RESUMEN
PURPOSE: The current study investigated the normative data values and repeatability of non-invasive tear film tests using Oculus Keratograph 5M in rabbit eyes. METHODS: Triplicate analysis of tear film parameters [tear meniscus height (TMH), non-invasive tear break up time (NIBUT), bulbar congestion, meibography] of 38 healthy adults New Zealand white rabbits (mean age, 13 ± 4.7 months) was performed using Oculus keratograph 5M (K5M). Bland-Altman analysis was used for testing repeatability. RESULTS: Thirty-eight rabbits (N = 76 eyes) weighing 2.5 ± 0.4 kg were studied. The mean NIBUT values of the right and left eye were 14.3 ± 5.8 and 12.3 ± 5.8 s, respectively. The mean central TMH values were 0.43 ± 0.23 and 0.33 ± 0.14 mm in the right and left eye, respectively. Meibography showed closely placed, wide meibomian glands running vertically with no distorted or gland loss areas. There were no differences between the eyes for the above parameters. Bland-Altman plot showed good repeatability for both NIBUT and TMH values. CONCLUSION: The non-invasive tear film parameters show good repeatability using keratograph 5M and can be used as an objective parameter for rabbit ocular surface experiments.
Asunto(s)
Síndromes de Ojo Seco , Adulto , Humanos , Conejos , Animales , Lactante , Síndromes de Ojo Seco/diagnóstico , Examen Físico , Lágrimas , Glándulas TarsalesRESUMEN
Dry eye disease (DED) is an emerging health issue affecting people worldwide. There have been rapid advances in the development of novel molecules and targeted therapies for the treatment of DED in the recent past. For testing and optimizing these therapies, it is necessary to have reliable experimental animal models of DED. One such approach is the use of benzalkonium chloride (BAC). Several BAC-induced DED models of rabbits and mice have been described in literature. BAC induces high levels of proinflammatory cytokines in the cornea and conjunctiva, along with epithelial cell apoptosis and reduction of mucins, which leads to tear film instability, thereby successfully simulating human DED. The stability of these models directs whether the treatment is to be applied while BAC is being instilled or after its cessation. In this review, we summarize the previously described BAC animal models of DED and present original data on rabbit DED models created using 0.1%, 0.15%, and 0.2% BAC administration twice daily for two consecutive weeks. The 0.2% BAC model sustained DED signs for 3 weeks, while 0.1% and 0.15% models sustained DED signs for 1-2 weeks after BAC discontinuation. Overall, these models look promising and continue to be used in various studies to investigate the efficacy of therapeutic drugs for DED treatment.