RESUMEN
Some people are more attractive to mosquitoes than others, but the mechanistic basis of this phenomenon is poorly understood. We tested mosquito attraction to human skin odor and identified people who are exceptionally attractive or unattractive to mosquitoes. These differences were stable over several years. Chemical analysis revealed that highly attractive people produce significantly more carboxylic acids in their skin emanations. Mutant mosquitoes lacking the chemosensory co-receptors Ir8a, Ir25a, or Ir76b were severely impaired in attraction to human scent, but retained the ability to differentiate highly and weakly attractive people. The link between elevated carboxylic acids in "mosquito-magnet" human skin odor and phenotypes of genetic mutations in carboxylic acid receptors suggests that such compounds contribute to differential mosquito attraction. Understanding why some humans are more attractive than others provides insights into what skin odorants are most important to the mosquito and could inform the development of more effective repellents.
Asunto(s)
Aedes , Anopheles , Repelentes de Insectos , Animales , Humanos , Ácidos Carboxílicos/farmacología , Odorantes/análisis , Repelentes de Insectos/farmacología , Repelentes de Insectos/análisisRESUMEN
Human health and disease have increasingly been shown to be impacted by the gut microbiota, and mouse models are essential for investigating these effects. However, the compositions of human and mouse gut microbiotas are distinct, limiting translation of microbiota research between these hosts. To address this, we constructed the Mouse Gastrointestinal Bacteria Catalogue (MGBC), a repository of 26,640 high-quality mouse microbiota-derived bacterial genomes. This catalog enables species-level analyses for mapping functions of interest and identifying functionally equivalent taxa between the microbiotas of humans and mice. We have complemented this with a publicly deposited collection of 223 bacterial isolates, including 62 previously uncultured species, to facilitate experimental investigation of individual commensal bacteria functions in vitro and in vivo. Together, these resources provide the ability to identify and test functionally equivalent members of the host-specific gut microbiotas of humans and mice and support the informed use of mouse models in human microbiota research.
Asunto(s)
Bacterias/clasificación , Bacterias/aislamiento & purificación , Microbioma Gastrointestinal/fisiología , Animales , Bacterias/genética , Bacterias/metabolismo , Butiratos/metabolismo , Genoma Bacteriano , Humanos , Metagenoma/genética , Ratones , Modelos AnimalesRESUMEN
The dissolution of silver nanoparticles (AgNPs) to release Ag(I)(aq) is an important mechanism in potentiating AgNP cytotoxicity and imparting their antibacterial properties. However, AgNPs can undergo other simultaneous biophysicochemical transformations, such as protein adsorption, which can mediate AgNP dissolution behaviors. We report the comprehensive analysis of AgNP dissolution and protein adsorption behaviors with monolayer surface coverage of AgNPs by bovine serum albumin (BSA). AgNP dissolution rate constants, kdissolution, were quantified over several particle sizes (10, 20, and 40 nm) and BSA concentrations (0-2 nM) using linear sweep stripping voltammetry. Across all particle sizes, the dissolution rate constant increased with increasing BSA concentrations. However, protein-enhanced dissolution behaviors were most pronounced for 10 nm AgNPs, which exhibited 3.6-fold and 7.7-fold relative enhancement when compared to 20 and 40 nm AgNPs, respectively. Changes to AgNP surface properties upon interaction with BSA were monitored using dynamic light scattering and zeta potential measurements, while BSA-AgNP complex formation was evaluated using UV-vis spectroscopy and circular dichroism spectroscopy. A subtle increase in the BSA-AgNP association constant was observed with an increase in the AgNP size. Together, these results suggest that the AgNP size dependence of BSA-enhanced dissolution of AgNPs is possibly mediated through both displacement of Ag(I)(aq)-loaded BSA by excess protein in the bulk solution and minimized accessibility of the AgNP surface because of BSA adsorption.