RESUMEN
The genotypes of hepatitis B (HBV) and delta (HDV) viruses circulating among Venezuelan Amerindian populations, where these viruses are endemic, were determined by sequencing of PCR amplified products from HBsAg positive sera. HDV genotype I (n = 7, 6 from West Amerindians), and III (n = 5, 4 from South Amerindians), were found. Only one HDV genotype I isolate was associated with HBV genotype D, 4 HDV genotype I and 2 HDV genotype III infected individuals were co-infected with HBV genotype F. The failure to detect the South American HDV genotype III in West Amerindians might be related to the outbreak of fulminant hepatitis with high mortality rate occurred between 1979 and 1982, probably affecting more the Amerindians infected with HDV genotype III. These results suggest the circulation of HDV genotype I among Amerindians, probably introduced through European immigrations, and that this HDV genotype is able to replicate in association with HBV genotype F.
Asunto(s)
Biomarcadores/análisis , ADN Viral/análisis , Anticuerpos Antihepatitis/análisis , Virus de la Hepatitis B/genética , Hepatitis D Crónica/complicaciones , Hepatitis D/complicaciones , Hepatitis D/genética , Virus de la Hepatitis Delta/genética , Indígenas Sudamericanos/genética , ARN Viral/análisis , Secuencia de Aminoácidos , Secuencia de Bases , Biomarcadores/sangre , ADN Viral/sangre , Genotipo , Anticuerpos Antihepatitis/sangre , Anticuerpos Antihepatitis/inmunología , Hepatitis B/sangre , Hepatitis B/virología , Antígenos de Superficie de la Hepatitis B/análisis , Antígenos de Superficie de la Hepatitis B/sangre , Virus de la Hepatitis B/aislamiento & purificación , Hepatitis D/sangre , Hepatitis D/epidemiología , Hepatitis D/virología , Hepatitis D Crónica/sangre , Hepatitis D Crónica/epidemiología , Hepatitis D Crónica/virología , Virus de la Hepatitis Delta/aislamiento & purificación , Humanos , Indígenas Sudamericanos/clasificación , Filogenia , Reacción en Cadena de la Polimerasa , ARN Viral/sangre , Alineación de Secuencia , Venezuela/epidemiologíaRESUMEN
Diarrhea is one of the most common problem of public health worldwide, specially in developing countries. In Venezuela, this affection must be weekly reported when it occurs in children under five years of age. During June 1993 to May 1995, 379 stool specimens were obtained from children under five years of age with diarrhea admitted in Hospital de Niños of Maracaibo, Venezuela. Control group was conformed by 93 asymptomatic children. The rotavirus RNA was extracted with phenol-chloroform and precipitated with ethanol. Finally, polyacrylamide-gel-electrophoresis (PAGE), followed by silver staining was employed for rotavirus detection. Our results showed 62 (16.4%) positive cases for rotavirus in symptomatic children and 9 (9.7%) in control group. The highest incidence was showed in the infants under one year of age (21.5%). The electrophoretic analysis revealed only one long electropherotype pattern in studied samples. It was observed a close relationship between the increase of rotavirus cases and the increase of the pluviometric index and a decrease of mean temperature observed during the period of study. Both variables determine the increase or decrease of viral infection in our region. This result shows the importance of the climatic factors in the rotavirus epidemiology.
Asunto(s)
Diarrea Infantil/epidemiología , Conceptos Meteorológicos , Infecciones por Rotavirus/epidemiología , Preescolar , Diarrea Infantil/virología , Brotes de Enfermedades , Femenino , Humanos , Incidencia , Lactante , Recién Nacido , Masculino , ARN Viral/análisis , Lluvia , Temperatura , Clima Tropical , Salud Urbana , Venezuela/epidemiologíaRESUMEN
The adw4 subtype of hepatitis B virus (HBV) belongs to a unique genomic group (genotype F) representing the original HBV strains from the New World. Data regarding the prevalence of this subtype among HBV carriers in South America are, however, scarce, and those concerning HBV genotype F are based on only a few samples from Latin America. In this study, serum samples were obtained from 141 hepatitis B surface antigen (HBsAg) carriers from Amerindians and urban populations from Venezuela. The HBsAg subtype was identified with monoclonal antibodies in 105 samples, and the HBV genotype was identified by reverse-phase hybridization with DNA fragments in 58 samples. The adw4 subtype was highly prevalent in the population studied (75%); among the Amerindians, the prevalence was 97%. The adw2 subtype was also present (10%), while other subtypes (ayw3 and ayw4) were only occasionally found. The HBV subtype was associated with the expected genotype in most cases (80%), and thus genotype F was highly prevalent. Sequencing of viral strains that gave genotypes unpredicted by the HBsAg subtyping confirmed seven of them as belonging to not previously described genotype-subtype associations: namely, adw2 and ayw4 within genotype F.
Asunto(s)
Variación Antigénica , Antígenos de Superficie de la Hepatitis B/genética , Virus de la Hepatitis B/genética , Hepatitis B/virología , Indígenas Sudamericanos , Sustitución de Aminoácidos , Femenino , Genotipo , Hepatitis B/epidemiología , Hepatitis B/etnología , Antígenos de Superficie de la Hepatitis B/inmunología , Virus de la Hepatitis B/clasificación , Virus de la Hepatitis B/inmunología , Humanos , Embarazo , Complicaciones Infecciosas del Embarazo/virología , Análisis de Secuencia , Venezuela/epidemiologíaRESUMEN
Recently, a new virus related to flaviviruses, the hepatitis G virus (HGV), or GBV-C virus, was discovered as a putative blood-borne human pathogen. HGV RNA (NS5 region) was amplified by reverse transcription-nested PCR in the sera of 6 of 64 (9%) hemodialysis patients; 2 of 80 (2.5%) West Yukpa Amerindians, a population with a high rate of HBV infection but negative for HCV infection; and 1 patient with an acute episode of non-A, non-B, non-C hepatitis (NABCH). The patterns of single-strand conformation polymorphism of the amplified products were unique among different specimens and similar on follow-up for hemodialysis patients. All patients tested remained HGV RNA positive 1 and 2 years later, without major sequence variation, except for the NABCH patient, for whom a double infection and an apparent clearance of the original dominant variant was observed after 2 years. The sequences of the NS5 amplified products demonstrated 85 to 90% identity with other reported HGV sequences.
Asunto(s)
Flaviviridae/aislamiento & purificación , Hepatitis Viral Humana/diagnóstico , Hepatitis Viral Humana/epidemiología , ARN Viral/sangre , ARN Viral/aislamiento & purificación , Secuencia de Bases , Clonación Molecular , Flaviviridae/genética , Hepatitis C/diagnóstico , Hepatitis E/diagnóstico , Hepatitis Viral Humana/sangre , Humanos , Indígenas Sudamericanos , Datos de Secuencia Molecular , Filogenia , Reacción en Cadena de la Polimerasa , Polimorfismo de Longitud del Fragmento de Restricción , Polimorfismo Conformacional Retorcido-Simple , ARN Viral/genética , Diálisis Renal/efectos adversos , Análisis de Secuencia de ARN , Venezuela/epidemiologíaRESUMEN
Hepatitis C virus genotypes in Venezuela were analyzed by restriction fragment length polymorphism in the 5' noncoding region. The absence of BstUI digestion was found to be a useful marker for genotype 2 specimens. From 122 serum samples, 66, 20, and 2.5% were classified as genotypes 1, 2, and 3, respectively; 0.8% were classified as genotype 4; and 10% appeared to be mixed infections.
Asunto(s)
Hepacivirus/genética , Hepatitis C/virología , Genes Virales/genética , Genotipo , Hepacivirus/aislamiento & purificación , Hepatitis C/epidemiología , Humanos , Datos de Secuencia Molecular , Venezuela/epidemiologíaRESUMEN
Antibody reactivities to hepatitis C virus (HCV) antigens and to synthetic peptides derived from different parts of the HCV genome (core, NS4, and NS5) were evaluated in HCV-infected hemodialysis patients. In the RIBA 3 assay, NS5 was significantly less recognizable by sera of hemodialysis patients compared to other HCV-infected subjects. Among hemodialysis patients, those coinfected with hepatitis B virus (HBV) (positive for hepatitis B surface antigen [HBsAg+]) showed a reduction in reactivity to C33 and C100. Sera of only 23% of the hemodialysis patients (37 of 161) reacted with more than three of eight peptides tested, significantly fewer than the 60% (12 of 20) of the sera of other HCV-infected patients tested (P = 0.001). This immunosuppression was also manifested by a reduced frequency of recognition of additional peptides on follow-up. An even more reduced reactivity was observed among the HBV-coinfected patients (HBsAg+). The low-responder hemodialysis patients were not infected with any particular genotype of HCV, and the same HCV genotypes observed in the whole group of hemodialysis patients (1a, 1b, 2a, and 3a) were found circulating in the low-responder group. Even in this low-responder population, the good performance of two peptides (peptide 716, corresponding to a portion of the core, and peptide 59, corresponding to a portion of NS4) corroborates the immunodominance of the conserved epitopes within these peptides.