RESUMEN
OBJECTIVE: Assessment of antibacterial properties of a new intracanal paste based on calcium hydroxocuprate (CHC) and silver nanoparticles hydrosol for passive root impregnation. MATERIAL AND METHODS: The study included 55 teeth with 69 root canals belonging patients with chronic apical periodontitis. The main group, including 44 root canals, was filled with a new paste based on CHC and silver nanoparticles for 7 days after preparation and irrigation. In the control group, 25 root canals were sealed with an aqueous paste of calcium hydroxide for 14 days. The presence of the endodontic microorganisms was evaluated by real-time PCR. RESULTS AND DISCUSSION: Further analysis showed that the amount of the DNA, common for P. gingivalis, T. forsythia and T. denticola, after treatment was less in the main group, where the new paste was applied. These results were significant at the p=0.05 level (p=0.005, p=0.006, p=0.003 according to each mentioned bacterial sample). No significant differences were found between the groups in the number of genome equivalents specific for P. intermedia and F. nucleatum (p=0.543, p=0.554). CONCLUSION: These findings suggest that the new method of passive root impregnation with the CHC and silver nanoparticles paste may be an effective method for the treatment of chronical apical periodontitis.
Asunto(s)
Nanopartículas del Metal , Periodontitis Periapical , Humanos , Reacción en Cadena en Tiempo Real de la Polimerasa , Nanopartículas del Metal/uso terapéutico , Plata/farmacología , Calcio , Antibacterianos/farmacología , Antibacterianos/uso terapéutico , Periodontitis Periapical/tratamiento farmacológico , Raíz del Diente , DentinaRESUMEN
THE AIM: Was to study the promising areas for using nanotechnologies in dentistry, existing methods of diagnostics, treatment and prevention of the dental diseases based on the properties of nanoparticles, to review the scientific literature devoted to this problem. In this literature review we use 86 sources: 1 Russian and 85 foreign articles. Analyzed articles were published within the last 5 years. The literature review summarizes and presents up-to-date methods of diagnosing, treating, and preventing dental disease that use nanotechnologies. Development and implementation of nanotechnological treatment are a promising direction for modern dentistry.
Asunto(s)
Nanoestructuras , Odontología , Humanos , Federación de RusiaRESUMEN
In order to obtain reliable information about time-domain ECG parameters in Russian children and to analyse their links with physiological changes the Project "ECG screening of children and adolescents of the Russian Federation" was carried out in 2003-2008. Time domain ECG parameters were studied in the representative sample of 5387 healthy individuals aged 0 to 18 years. In each age group, lower and upper limits of the norm for heart rate (HR), P wave, PQ and QRS intervals were defined as 2nd and 98th percentiles of their distribution. The equivalent limits of QT interval duration were defined as its 5th and 95th percentiles. Relationship between time domain parameters of pediatric ECG and HR was analyzed as well as relationship of these parameters with sex and age. The most important differences from prior pediatric studies completed 30 years ago were obtained for the heart rate limits. Among children aged 0 to 3, there was a tendency toward lowering of age-specific lower limits and elevation of age-specific upper limits. At ages older than 3 years, there was a tendency toward lowering of both the upper and the lower limits of HR. The newly obtained 2nd percentiles of the heart rate were lower than the known lower limits in nearly all ages. This indicates the necessity for revision of criteria of bradicardia in children. The present study showed that 5th and 95th percentiles of QTc interval (Bazett's formula) varied between 340 ms and 465 ms and were not significantly dependent on children's age and only for ages under 13 also on sex. Results of the study allowed to build age-specific ranges of norm for the time domain ECG parameters presented in a tabular form.