RESUMEN
The novel HLA-DPA1*01:195 allele differs from HLA-DPA1*01:03:01:01 by one nucleotide substitution in exon 4.
Asunto(s)
Alelos , Exones , Cadenas alfa de HLA-DP , Secuenciación de Nucleótidos de Alto Rendimiento , Prueba de Histocompatibilidad , Humanos , Cadenas alfa de HLA-DP/genética , Secuenciación de Nucleótidos de Alto Rendimiento/métodos , Prueba de Histocompatibilidad/métodos , Secuencia de Bases , Análisis de Secuencia de ADN/métodos , Polimorfismo de Nucleótido Simple , Alineación de SecuenciaRESUMEN
The novel HLA class II allele HLA-DPB1*1485:01 is described.
Asunto(s)
Médula Ósea , Donantes de Tejidos , Humanos , Alelos , Cadenas beta de HLA-DP/genéticaRESUMEN
The novel HLA class II allele HLA-DPB1*1326:01 is described.
Asunto(s)
Médula Ósea , Donantes de Tejidos , Humanos , Alelos , Cadenas beta de HLA-DP/genéticaRESUMEN
Sedimentation has a prominent impact on the functionality and lifetime of reservoirs and is a growing concern for stakeholders. Various parameters influence sedimentation caused by soil erosion. Here we have examined fifty Italian reservoirs to determine sedimentation rates and storage capacity loss. The reservoirs studied have an average age of 78 years as of 2021, with the highest loss of capacity observed, equal to 100%, for Ceppo Morelli. For the fifty Italian catchments covering north, south, central and islands of Italy, we found the mean annual sediment yield varying between 17-4000 m3/km2. year. Six of fifty reservoirs studied (Quarto, Colombara, Ceppo Morelli, Fusino, Vodo and Valle di Cadore) are already in a very critical situation in terms of storage capacity loss. Out of the fifty reservoirs, half of them will reach their half-life year by 2050. For example, for the Fusino reservoir located in northern Italy, we observed a loss of 90% of the storage volume as of 2020 with respect to its operation year 1974, compared to 6% in 2015 as available in literature. Modelling the sediment delivery ratio (SDR) is an open question, due to the lack of adequate data and uncertainties about the variability in hydrological, geomorphological, climate and landcover parameters. Here, we addressed the issue with a simplified multiple regression approach based on sediment delivery ratio values retrieved by the RUSLE model. We found different multi regressions for reservoirs belonging to the Alpine and Apennine regions. This analysis offers a starting point for the management and prioritization of adaptation and remediation policies necessary to address reservoir sedimentation.
Asunto(s)
Sedimentos Geológicos , Suelo , Monitoreo del Ambiente/métodos , ItaliaRESUMEN
BACKGROUND: Redondovirus (ReDoV) is a recently discovered circular, Rep-encoding single-stranded DNA (CRESS-DNA) virus in humans. Its pathogenesis and clinical associations are still completely unknown. METHODS: The presence of ReDoV DNA was investigated in biological specimens of 543 Italian subjects by in-house developed PCR assays. RESULTS: The overall ReDoV prevalence was about 4% (23 of 543 samples). The virus was detected in 22 of 209 (11 %) respiratory samples. One stool sample was also ReDoV positive. Viral DNA was not found in blood samples from immunocompetent and immunosuppressed subjects and cerebrospinal fluids from patients with neurological diseases. Genomic nucleotide differences were detected among the ReDoV isolates by sequencing a 582-nucleotide fragment of the capsid gene of the viral genome. CONCLUSIONS: The results demonstrate that ReDoV is mainly present in the respiratory tract of infected people. Further investigations are needed to reveal possible clinical implications of this new CRESS-DNA virus in humans.
Asunto(s)
Infecciones por Virus ADN/virología , Virus ADN/aislamiento & purificación , Infecciones del Sistema Respiratorio/virología , Adulto , Anciano , Proteínas de la Cápside/genética , Infecciones por Virus ADN/epidemiología , Virus ADN/clasificación , Virus ADN/genética , ADN Viral/genética , Heces/virología , Femenino , Variación Genética , Genoma Viral/genética , Humanos , Italia/epidemiología , Masculino , Persona de Mediana Edad , Filogenia , Prevalencia , Infecciones del Sistema Respiratorio/epidemiología , Estudios Retrospectivos , Análisis de Secuencia de ADNRESUMEN
Second-order rate constants of the aminolysis of 2-cyano-2-phenylpropanoic anhydride 3 by a series of N-methylanilines differently substituted in the aromatic moiety (4a-d) were measured in dichloromethane. The common reaction product of aminolysis is 2-cyano-2-phenylpropanoic acid 1, which is known to be an effective fuel for acid-base driven molecular machines, but cannot be used in molar excess with respect to the machine. The motivation behind the kinetic study has been the prospect of using the aminolysis of 3 to supply the machine with fuel at a rate that is never so high as to overfeed the system, thus avoiding the malfunction of the machine with concomitant waste of fuel. Knowledge of the kinetic parameters dictated the choice of 4c as the best nucleophile in the lot for feeding acid 1 into a catenane-based molecular machine at a rate that ensured a correct operation.
RESUMEN
Natural molecular machines require a continuous fuel supply to perform motions and/or remain in a functional state. Consequently, the aim of developing artificial devices and materials with life-type properties has motivated a growing interest in abiotic chemical fuels and in their supply modalities. Many artificial molecular machines have been developed in which the sequential addition of several chemical reagents allows the machine to perform complete cycles of motion. Only recently, examples of molecular machines whose cycles of motion are triggered by a single pulse of fuel have been reported. The latter systems are the object of this Minireview where the abiotic chemical fuels used so far to trigger the complete cycles of motion of molecular machines are described, with particular emphasis on the operation mechanism of the machine/fuel systems.
RESUMEN
This work aimed to render phenomenologically autonomous the otherwise stepwise operation of a catenane-based molecular switch, which is chemically triggered by the decarboxylation of 2-cyano-2-phenylpropanoic acid (2). Given that any amount of 2 in stoichiometric excess with respect to the catenane is consumed in a side reaction, the authors resorted to the corresponding anhydride 5, the slow hydrolysis of which, due to adventitious water in dichloromethane, continuously produces in situ the actual fuel 2. As a consequence, the machine does not require a reloading after each cycle, but switches back and forth as long as fuel is present.
RESUMEN
The back and forth motions of a crown-ether based wheel along the axis of a bistable rotaxane are triggered by the decarboxylation of 2-cyano-2-phenylpropanoic acid and detected by the oscillation of the EPR nitrogen splitting of a dialkyl nitroxide function mounted within the macrocyclic ring. When the p-Cl derivative of the acid is used, back and forth motions are accelerated. Conversely, with p-CH3 and p-OCH3 derivatives, the back motion is strongly inhibited by the insurgence of collateral radical reactions.
RESUMEN
We report on catalysis by a fuel-induced transient state of a synthetic molecular machine. A [2]rotaxane molecular shuttle containing secondary ammonium/amine and thiourea stations is converted between catalytically inactive and active states by pulses of a chemical fuel (trichloroacetic acid), which is itself decomposed by the machine and/or the presence of additional base. The ON-state of the rotaxane catalyzes the reduction of a nitrostyrene by transfer hydrogenation. By varying the amount of fuel added, the lifetime of the rotaxane ON-state can be regulated and temporal control of catalysis achieved. The system can be pulsed with chemical fuel several times in succession, with each pulse activating catalysis for a time period determined by the amount of fuel added. Dissipative catalysis by synthetic molecular machines has implications for the future design of networks that feature communication and signaling between the components.
RESUMEN
Two adjacent nucleotide substitutions in exon 2 characterize the novel class II HLA-DPB1*687:01 allele.
Asunto(s)
Exones , Cadenas beta de HLA-DP/genética , Prueba de Histocompatibilidad/métodos , Trasplante de Riñón , Polimorfismo de Nucleótido Simple , Análisis de Secuencia de ADN/métodos , Alelos , Sustitución de Aminoácidos , Secuencia de Bases , Humanos , Italia , Masculino , Homología de SecuenciaRESUMEN
This work deals with the use of 2-cyano-2-arylpropanoic acids as chemical fuels for an acid-base operated molecular switch that consists of a Sauvage-type catenand composed of two identical macrocycles incorporating a phenanthroline unit. When used as a base promoter of the decarboxylation of propanoic acid derivatives, the switch undergoes large amplitude motion from the neutral catenand to a protonated catenate and back again to the neutral state. The rate of back proton transfer, which determines the rate of the overall process, was markedly affected by para-substituents in the order Cl > H > CH3 > OCH3 (ρ = +5.2). Thus, the time required to complete a full cycle was almost two days for the OCH3 derivative and dropped to a few minutes for the Cl derivative. These results show for the first time that the rate of operation of a molecular switch can be regulated by variations in the fuel structure.
RESUMEN
Back and forth motions of the acid-base-operated molecular switch 1 are photo-controlled by irradiation of a solution, which also contains the photolabile pre-fuel 4. The photo-stimulated deprotection of the pre-fuel produces controlled amounts of acid 2, the base-promoted decarboxylation of which fuels the back and forth motions of the Sauvage-type [2]-catenane-based molecular switch. Because switch 1 and pre-fuel 4 do not interact in the absence of irradiation, an excess of the latter with respect to 1 can be added to the solution from the beginning. In principle, photocontrol of the back and forth motions of any molecular machine, the operation of which is guided by protonation/deprotonation, could be attained by use of pre-fuel 4, or of any other protected acid that undergoes deprotection by irradiation with light at a proper wavelength, followed by decarboxylation under conveniently mild conditions.
RESUMEN
The decarboxylation of 2-cyano-2-phenylpropanoic acid is fast and quantitative when carried out in the presence of 1â molar equivalent of a [2]catenane composed of two identical macrocycles incorporating a 1,10-phenanthroline unit in their backbone. When decarboxylation is over, all of the catenane molecules have experienced large-amplitude motions from neutral to protonated catenane, and back again to the neutral form, so that they are ready to perform another cycle. This study provides the first example of the cyclic operation of a molecular switch at the sole expenses of the energy supplied by the substrate undergoing chemical transformation, without recourse to additional stimuli.