RESUMEN
Here, we report the physicochemical interaction among iron oxide nanoparticles (MNPs) and essential biomolecules, namely, serum albumin (BSA, HSA), collagen and deoxyribonucleic acid (DNA) in the presence of various cationic, anionic and non-ionic surfactants. Iron oxide nanoparticles are synthesized by the wet chemical process and are characterized by X-ray powder diffraction analysis (XRD), Fourier transform infrared spectroscopic, UV-Vis spectroscopy, scanning electron microscopy and energy-dispersive X-ray spectroscopy mapping studies . The conjugation of MNPs protein was analyzed using UV-Vis spectroscopy, fluorescence spectroscopy, circular dichroism technique and gel electrophoresis. The spectroscopic investigation illustrates the surfactant-dependent binding between MNPs and protein. Gel electrophoresis in the absence and presence of MNPs-surfactant systems has been used to study the impact on DNA structure. It was found that Tween 80 imparts better stability as well as biocompatibility to the synthesized MNPs. The findings offer extensive information on the influence of various surfactant coatings on MNP surfaces and their influence on vital biomolecules, making it useful for designing MNPs for biological applications.Communicated by Ramaswamy H. Sarma.
Asunto(s)
Nanopartículas de Magnetita , Tensoactivos , Tensoactivos/química , Nanopartículas de Magnetita/química , Albúmina Sérica , Fenómenos Químicos , ADN/química , Espectroscopía Infrarroja por Transformada de FourierRESUMEN
Thymoquinone (TQ) is a bioactive component of medicinal plant, Nigella sativa. It has been identified as promising anti-inflammatory and anti-analgesic properties. In the present study, the TQ has been investigated for physiological interaction as well as binding properties with serum albumin and their thermodynamic parameters at different temperatures. Glycation process was checked with the measurement of fructosamine content, carbonyl content and total advanced glycated end products. The aggregation of amyloid ß-structure was measured with Thioflavin-T and the secondary structure of BSA was observed by circular dichroism (CD) in glycated and thermal treated samples. The results indicate that the TQ showed binding interaction (both static and dynamic) with BSA (Kb= 18.31 × 107 M-1 at 293 K) and suppression of glycated products. The glycation-induced and thermal aggregation were prevented and the secondary structure of BSA was maintained. Therefore, these findings suggest that TQ may be used for a therapeutic drug for antiglycation as well as anti-aggregation.Communicated by Ramaswamy H. Sarma.
Asunto(s)
Péptidos beta-Amiloides , Albúmina Sérica Bovina , Benzoquinonas , Fructosamina , Productos Finales de Glicación Avanzada/metabolismo , Albúmina Sérica , Albúmina Sérica Bovina/químicaRESUMEN
Nanoparticles have been proven to be a great tool as bio-sensors, medical therapeutic agents and drug delivery vehicles. In this study, the chemically synthesized zinc oxide nanoparticles (ZnO NPs) have been characterized with UV-spectrophotometer, FTIR, XRD, TEM and DLS. These ZnO NPs were investigated with respect to their binding interaction with serum albumin and the thermodynamic parameters of these interactions at different temperatures. Glycation process was checked in the presence of ZnO NPs by measuring fructosamine and carbonyl content for glycated end products and aggregation by Congo red assay. The intrinsic activities of bovine serum albumin (BSA) like esterase and cysteine reactivity were also evaluated in the presence of ZnO NPs. The results indicate that the ZnO NPs showed static as well as dynamic binding interaction with BSA, reduced the content of glycation products and prevented the glycation induced aggregation and antioxidant properties. Therefore, these findings suggest that ZnO NPs may be used for drug delivery agents and antiglycating as well as an antioxidant agent.Communicated by Ramaswamy H. Sarma.
Asunto(s)
Nanopartículas del Metal , Nanopartículas , Óxido de Zinc , Albúmina Sérica Bovina , EspectrofotometríaRESUMEN
The exponential industrial growth we see today rides on the back of large scale production of chemicals, explosives and pharmaceutical products. However, the effluents getting released from their manufacturing units are greatly compromising the sustainability of our environment. With greater awareness of the imperative for environmental clean-up, a promising approach that is attracting increasing research interests is biodegradation of xenobiotics. In this approach, biotransformation has proven to be one of the most effective tools. While many different model frameworks have been used to study different aspects of biotransformation, hairy roots (HRs) have been found to be exceptionally valuable. HR cultures are preferred over other in-vitro model systems due to their biochemical stability and hormone-autotrophy. In addition, the multi-enzyme biosynthetic potential of HRs which is similar to the parent plant and their relatively low-cost cultural requirements further characterize their suitability for biotransformation. The recent progress observed in scale-up of HR cultures and understanding of functional genomics has opened up new dimensions providing valuable insights for industrial application. This review article summarizes the potential of HR cultures in the biotransformation of xenobiotics, their limitations in the application on a large scale and current strategies to alleviate them. Advancement in bioreactors engineering enabling large scale cultivation and modern gene technologies improving biotransformation efficiency promises to extend laboratory results to industrial applications.