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Adoption of conservation agriculture (CA) is very slow due to weed infestations. The application of herbicides is the only viable option to deal with problem of weed management to adhere with basic principles of CA. A field experiment was carried out for three years to evaluate the expediency of different herbicides and their sequential applications under CA. In this study, seven treatments comprised of either alone or sequential application of pre-emergence (PE) and post-emergence (PoE) herbicides, hand weeding and weedy check were tested in soybean. Result indicated that sequential application of glyphosate at 1 kg ai ha-1 + pendimethalin at 1 kg ai ha-1as PE followed by PoE application of imazethapyr at 100 g ai ha-1 at 30 days after sowing (DAS) proved to be the best economical option in terms of plant growth parameters, crop biomass, seed yield, weed index and carbon and nutrient recycling. Pearson's correlation coefficients matrix revealed that grain yield was significantly (P<0.0001) related to weed density at harvest (r = -0.84), (WDH) (r = -0.63), weed dry biomass (WDB) (r = -0.52), weed nitrogen (N), phosphorus (P) and potassium (K) uptake (r = -0.56, r = -0.59 and r = -0.66), respectively and weed index (WI) (r = -0.96). The bivariate linear regression study of grain yield on weed control efficiency (WCI) biomass, N, P and K uptake by grain showed a clear significant (P<0.0001) dependence with R2 value of 0.53, 0.99, 0.95 and 0.98, respectively. The fitted stepwise multiple regression model also revealed that N and P uptake in grain, weed density at 20 DAS and K uptake in weed were actual predictor for grain yield. We concluded that, effective and economical weed control under CA in soybean can be achieved through sequential application of glyphosate along with pendimethalin at 1 kg ai ha-1 each PE followed by PoE use of imazethapyr at 100 g ai ha-1 at 30 DAS.

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We present a rare case of an intraorbital dermoid which was associated with a small temporal region dermal sinus in a 3-year-old child. This got infected and the child presented with orbital cellulitis. Definitive surgery involved excision of all the dermal elements using a superficial and intraorbital approach. We stress the need to evaluate, apparently benign lateral facial dermal sinuses as they may be the pointers of the underlying pathological deep dermoid cysts to avoid complications.

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The syndrome described by Zollinger and Ellison in 1955 is a rare clinical entity which is even rarer in children. This report describes a 12-year-old boy who presented with refractory peptic ulcer disease which was finally diagnosed to be due to a gastrinoma and was successfully treated.

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Most commonly inherited bleeding disorder, first described in Aland Islands by Erik von Willebrand. It occurs as a result of decrease in plasma levels or defect in von Willebrand factor which is a large multimeric glycoprotein. Monomers of this glycoprotein undergo N-glycosylation to form dimers which get arranged to give multimers. Binding with plasma proteins (especially factor VIII) is the main function of von Willebrand factor. The disease is of two forms: Inherited and acquired forms. Inherited forms are of three major types. They are type 1, type 2, and type 3; in which type 2 is sub-divided into 2A, 2B, 2M, 2N. Type 1 is more prevalent than all other types. Mucocutaneous bleeding is mild in type 1 whereas it is mild to moderate in types 2A, 2B, and 2M. Type 2N has similar symptoms of haemophilia. The pathophysiology of each type depends on the qualitative or quantitative defects in von Willebrand factor. The diagnosis is based on von Willebrand factor antigen, von Willebrand factor activity assay, FVIII coagulant activity and some other additional tests. Results should be analyzed within the context of blood group. von Willebrand factor multimer analysis is essential for typing and sub typing the disease. The management of the disease involves replacement therapy, non-replacement therapy and other therapies that include antifibrinolytics and topical agents.

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Influence of repeated application of the nitrification inhibitor dicyandiamide (DCD), on CH(4) production and associated microflora in a flooded alluvial soil, was investigated in a laboratory incubation study. Application of DCD at the time of soil incubation resulted in a substantial reduction in CH(4) production (31% over that of untreated control). Second repeat application of DCD, on the contrary, annulled the inhibitory effect on CH(4) production, restoring it to the level of unamended soil. Application of the third dose of DCD maintained CH(4) production almost to the same extent as that of second application. The alleviation of the initial inhibitory effect of DCD on CH(4) production was linked to the enhanced degradation of DCD following its repeated application to the flooded soil. Admittedly, abatement of the initial inhibitory effect of DCD on CH(4) production in soil repeatedly amended with DCD was also related to the inhibition of CH(4)-oxidizing bacterial population and noticeable stimulation of heterotrophic bacterial population. Results suggest that repeat application of DCD with fertilizer-N to flooded rice soils might not be effective in controlling CH(4) production under field condition.

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AIM: To improve the results of tubularized plate urethroplasty by adding de-epithelized flap. PATIENTS AND METHODS: Twenty-five cases of hypospadias who underwent Snodgrass urethroplasty using de-epithelialized flap were studied. The minimum period of follow-up in this series was 1 year. RESULTS: The resultant neo-meatus was vertically oriented and slit like. Glans was conical which is cosmetically well accepted. Penile raphe was in the midline. None of the patient had residual chordee, penile torsion, or glans dehiscence. Excellent cosmetic results were observed in all cases. The complication rate in our series was 8% (two cases). Two patients developed fistula. CONCLUSION: De-epithelialized flap is a simple method to provide additional covering to the constructed neourethra after Snodgrass urethroplasty. It achieves our goal of noncrossing suture lines and providing maximum vascularity.

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Aim: To improve the results of tubularized plate urethroplasty by adding de-epithelized flap. Patients and Methods: Twenty-five cases of hypospadias who underwent Snodgrass urethroplasty using de-epithelialized flap were studied. The minimum period of follow-up in this series was 1 year. Results: The resultant neo-meatus was vertically oriented and slit like. Glans was conical which is cosmetically well accepted. Penile raphe was in the midline. None of the patient had residual chordee; penile torsion; or glans dehiscence. Excellent cosmetic results were observed in all cases. The complication rate in our series was 8(two cases). Two patients developed fistula. Conclusion: De-epithelialized flap is a simple method to provide additional covering to the constructed neourethra after Snodgrass urethroplasty. It achieves our goal of noncrossing suture lines and providing maximum vascularity

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Nadi Pariksha has been said as one of the important Ashta sthana parikshas. Though, there are some physicians who are doing miracles just by examining Nadi, this system of examination in many views has became extinct now a days. The main reason for this is, now a days very little research and practice is going on regarding this technique. So, many resent samhitas and Chikitsa granthas like Sarangadhara, Yogaratnakara, etc., have highlighted Nadi pariksha. Basavarajiyam is also one of those resent treatises (19(th) century), which has highlighted this ancient type of examination of the patient. Basavarajiyam is a popular Ayurvedic heritage of Andhra Pradesh written by Basavaraju, son of Namahshivaya and disciple of Ramadeshika. The author has referred many books and collected many slokas from various ancient granthas and quoted them in 25 Prakaranas along with his own views and quotations. This book was translated in Telugu language by Puvvada surya Narayana Rao and lppublished by A.B.S.publications, Rajahmundry A.P. In this paper Nadi sthanas (Location), Nadi types, Nadi Pareeksha vidhi (examination of Nadi) and its utilities in different aspects of the disease are dealt, which have been mentioned in Basavarajeeyam.

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Envenoming by Echis saw scaled vipers and Bitis arietans puff adders is the leading cause of death and morbidity in Africa due to snake bite. Despite their medical importance, the composition and constituent functionality of venoms from these vipers remains poorly understood. Here, we report the cloning of cDNA sequences encoding seven clusters or isoforms of the haemostasis-disruptive C-type lectin (CTL) proteins from the venom glands of Echis ocellatus, E. pyramidum leakeyi, E. carinatus sochureki and B. arietans. All these CTL sequences encoded the cysteine scaffold that defines the carbohydrate-recognition domain of mammalian CTLs. All but one of the Echis and Bitis CTL sequences showed greater sequence similarity to the beta than alpha CTL subunits in venoms of related Asian and American vipers. Four of the new CTL clusters showed marked inter-cluster sequence conservation across all four viper species which were significantly different from that of previously published viper CTLs. The other three Echis and Bitis CTL clusters showed varying degrees of sequence similarity to published viper venom CTLs. Because viper venom CTLs exhibit a high degree of sequence similarity and yet exert profoundly different effects on the mammalian haemostatic system, no attempt was made to assign functionality to the new Echis and Bitis CTLs on the basis of sequence alone. The extraordinary level of inter-specific and inter-generic sequence conservation exhibited by the Echis and Bitis CTLs leads us to speculate that antibodies to representative molecules should neutralise the biological function of this important group of venom toxins in vipers that are distributed throughout Africa, the Middle East and the Indian subcontinent.

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Venom toxin-specific antibodies offer a more rational treatment of snake envenoming than conventional antivenom. Here, we describe novel cDNAs encoding phospholipase A(2) (PLA(2)) isoforms from venom gland RNA of Echis pyramidum leakeyi (Epl), Echis sochureki (Es) and Echis ocellatus (Eo). The deduced amino acid sequences of these cDNAs encoded proteins with high overall sequence identity to the viper group II PLA(2) protein family, including the 14 cysteine residues capable of forming seven disulphide bonds that characterize this group of PLA(2) enzymes. Comparison of the PLA(2) sequences from Echis with those from related vipers failed to make significant geographic, taxonomic or PLA(2)-function distinctions between these Echis PLA(2) isoforms. However, their deduced hydrophilicity profiles revealed a conserved tertiary structure that we will exploit, by epidermal DNA immunization, to generate PLA(2)-neutralizing antibodies with polyspecific potential.

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In a laboratory incubation study, methane (CH4) production in two saline soils and a nonsaline soil sample was investigated under flooded conditions. Mean CH4 production was remarkable (630.86 ng CH4/g) in nonsaline alluvial soil, but low (12.97 ng CH4/g) in acid sulfate saline (Pokkali) soil which was attributed to the high sulfate content of the later. CH4 production was also low in the coastal saline (Canning) soil (142.36 ng CH4/g) but increased upon leaching the soil of its salt content. Addition of salts to the nonsaline alluvial soil at 4, 8, 16 and 20 dS/m progressively decreased CH4 production. The inhibition of CH4 production was related to low microbial activities as reflected by decreased microbial biomass C and low soil microbial population including that of methanogens.

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In a laboratory incubation study, the effect of select heavy metals on methane (CH4) oxidation in two rice soils was investigated under two moisture regimes. Heavy metals differed in their effect on CH4 oxidation in both soils under the two water regimes. Cr significantly inhibited CH4 oxidation in the alluvial soil at 60% moisture holding capacity, while Cu stimulated the process. On the contrary, Zn inhibited CH4 oxidation in both alluvial and laterite soils only under flooded conditions. Application of rice straw alleviated the inhibitory effect of heavy metals on CH4 oxidation and CO2 production. Inhibition of CH4 oxidation in the alluvial soil was related to the methanotrophic bacterial population in Cr- and Zn-amended alluvial soil.

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In a laboratory incubation study, the effect of select heavy metals on methane (CH(4)) production in three rice soils was investigated under flooded conditions. Heavy metals behaved differently in their effect on methanogenesis in different soils and methane-producing bacteria. Cd, Cu, and Pb inhibited CH(4) production in all the soils. Zn stimulated CH(4) production in the alluvial soil, but inhibited it in laterite and acid sulfate soils. Cr effectively inhibited CH(4) production in the alluvial soil, but stimulated it in laterite and acid sulfate soils. The stimulatory effect of Zn and the inhibitory effect of Cr on methanogenesis in alluvial soil were attributed to their stimulation or inhibition of methanogenic bacterial population.

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