RESUMEN

Antimicrobial peptides are molecules synthesized by living organisms as the first line of defense against bacteria, fungi, parasites, or viruses. Since their biological activity is based on destabilization of the microbial membranes, a study of direct interaction forces between antimicrobial peptides and biomimetic membranes is very important for understanding the molecular mechanisms of their action. Herein, we use atomic force spectroscopy to probe the interaction between atomic force microscopy (AFM) tips functionalized with magainin 1 and supported lipid bilayers (SLBs) mimicking electrically uncharged membranes of normal eukaryotic cells and negatively charged membranes of bacterial cells. The investigations performed on negatively charged SLBs showed that the magainin 1 functionalized AFM tips are quickly adsorbed into the SLBs when they approach, while they adhere strongly to the lipid membrane when retracted. On contrary, same investigations performed on neutral SLBs showed mechanical resistance of the lipid membrane to the tip breakthrough and negligible adhesion force at detachment.

Asunto(s)

RESUMEN

Tantalum oxynitride thin films have been deposited by reactive magnetron sputtering, using a fixed proportion reactive gas mixture (85% N2 + 15% O2). To produce the films, the partial pressure of the mixture in the working atmosphere was varied. The characteristics of the produced films were analyzed from three main perspectives and correspondent correlations: the study of the bonding states in the films, the efficiency of photo-degradation, and the antibacterial/antibiofilm capacity of the coatings against Salmonella. X-ray Photoelectron Spectroscopy results suggest that nitride and oxynitride features agree with a constant behavior relative to the tantalum chemistry. The coatings deposited with a higher reactive gas mixture partial pressure exhibit a significantly better antibiofilm capacity. Favorable antibacterial resistance was correlated with the presence of dominant oxynitride contributions. The photocatalytic ability of the deposited films was assessed by measuring the level of degradation of an aqueous solution containing methyl orange, with or without the addition of H2O2, under UV or VIS irradiation. Degradation efficiencies as high as 82% have been obtained, suggesting that tantalum oxynitride films, obtained in certain configurations, are promising materials for the photodegradation of organic pollutants (dyes).

RESUMEN

A comprehensive assessment of the toxicity and antioxidant activity of cork boiling wastewater and the effects of gamma radiation on these parameters was performed. Antioxidant activity was evaluated using different methodologies as DPPH radical scavenging activity, reducing power and inhibition of ß-carotene bleaching. The results have shown that gamma radiation can induce an increase on the antioxidant activity of cork boiling wastewater. Toxicity tests were performed to access the potential added value of the irradiated wastewaters and/or minimization of the impact for discharge in the environment. Two different methods for toxicity evaluation were followed, bacterial growth inhibition test and cytotoxicity assay, in order to predict the behavior of different cells (prokaryotic and eukaryotic) in the presence of cork wastewater. Non-treated cork boiling wastewater seemed to be non-toxic for prokaryotic cells (Pseudomonas fluorescens and Bacillus subtilis) but toxic for eukaryotic cells (A549 human cells and RAW264.7 mouse cells). The gamma radiation treatment at doses of 100 kGy appeared to increase the toxicity of cork compounds for all tested cells, which could be related to a toxic effect of radiolytic products of cork compounds in the wastewaters.

Asunto(s)