RESUMEN
The aim of the study was a comparative analysis of diagnostic value of different laboratoty methods conducted on the basis of results of examination of patients during Legionnaires' disease outbreak in town Verkhnyaya Pyshma. Retrospective analysis of laboratory data from 74 patients with diagnosis of Legionnaires' disease was performed. Complex of laboratory methods was used (polymerase chain reaction (PCR), enzyme immunoassay (EIA), immunochromatography). In group of patients with Legionnaires' disease, the highest proportion of positive results (73%) was obtained by the EIA determining total specific antibodies in urine. Determination of antigen in urine by immunochromatographic express-test yielded 52% of positive results. PCR testing of blood specimens yielded positive results in 65% of samples but was low specific, due to that in 19% of patients from control group false-positive results were obtained. Testing of 3 autopsy samples showed that all specimens contained DNA of the causative agent. Performed analysis allowed to recommend complex use of immunochromatographic express-test of antigen detection and identification of total specific antibodies by EIA during mass people examination.
Asunto(s)
Cromatografía de Afinidad , Técnicas para Inmunoenzimas , Legionella , Legionelosis/diagnóstico , Reacción en Cadena de la Polimerasa , Anticuerpos Antibacterianos/sangre , Anticuerpos Antibacterianos/orina , Antígenos Bacterianos/orina , ADN Bacteriano/análisis , ADN Bacteriano/genética , Reacciones Falso Positivas , Humanos , Legionella/genética , Legionella/inmunología , Legionelosis/sangre , Legionelosis/orina , Estudios Retrospectivos , Federación de Rusia , Sensibilidad y EspecificidadRESUMEN
Issues of reliability, uniformity, and timeliness of reporting and sharing of information during outbreaks of infectious diseases are basic for establishment of tactics and strategy of organizational, antiepidemic, and clinical measures. Analysis of experience in sharing of information between medical care organization service and services ensuring sanitary-epidemiological welfare of population during outbreak of Legionnaires' disease in town Verkhnyaya Pyshma in July-August 2007 was performed in the article.
Asunto(s)
Personal Administrativo , Enfermedades Transmisibles Emergentes/prevención & control , Infecciones Comunitarias Adquiridas/prevención & control , Brotes de Enfermedades , Servicios de Información/organización & administración , Enfermedad de los Legionarios/prevención & control , Gobierno Local , Comportamiento del Consumidor , Conducta Cooperativa , Hospitales Urbanos , Humanos , Federación de Rusia/epidemiologíaRESUMEN
The aim of the study was to evaluate the dynamics of T-lymphocyte cytokine profile during the acute period of tick-bore encephalitis (febrile and meningeal forms) and non-erythematous Lyme borreliosis (NELB). ELISA (Vector-Best, Novosybirsk) and IHA (Virion, Tomsk) techniques were used for laboratory diagnostics of tick-borne encephalitis (TBE). Etiological verification of Lyme borreliosis (LB) was performed using immune-enzyme test systems for detection of IgG and IgM antibodies to Borellia burgdorferi (NOVATEC Immunodiagnostica, Germany). Thirty-eight subjects constituted the meningeal TBE group; the febrile TBE group consisted of 25 subjects. NELB was diagnosed in 20 subjects. Immunological tests were performed on days 1-7, 8-14, 15-21, and 22-28 of the disease. The study found that primary T-lymphocytes prevailed at early stages during the acute period of febrile TBE. In NELB, secondary T-lymphocytes were registered beginning from days 15-21. Meningeal TBE was characterized by a deep reduction in IFNgamma-producing CD3+ lymphocyte reserve.
Asunto(s)
Anticuerpos Antibacterianos/sangre , Borrelia burgdorferi/inmunología , Citocinas/inmunología , Encefalitis Transmitida por Garrapatas/inmunología , Inmunoglobulina G/sangre , Inmunoglobulina M/sangre , Enfermedad de Lyme/inmunología , Linfocitos T/inmunología , Enfermedad Aguda , Adulto , Citocinas/biosíntesis , Encefalitis Transmitida por Garrapatas/diagnóstico , Ensayo de Inmunoadsorción Enzimática , Humanos , Pruebas Inmunológicas , Interferón gamma/biosíntesis , Interferón gamma/inmunología , Interleucina-4/biosíntesis , Interleucina-4/inmunología , Enfermedad de Lyme/diagnóstico , Persona de Mediana Edad , Estadísticas no Paramétricas , Linfocitos T/metabolismo , Factores de TiempoRESUMEN
The authors propose a comprehensive approach to laboratory diagnosis of seasonal transmissible infections, based on modern methods permitting etiological deciphering of disease. A universal diagnostic algorithm notably accelerated the laboratory diagnosis due to cutting the period between collection of material from a patient and consecutive screening for antibodies to agents of tick-borne encephalitis, Lyme disease, and California encephalitis.