RESUMEN

The first objective of this study was to determine the GenoType NTM-DR assay performance for subspecies identification in Mycobacterium abscessus complex isolates. The second objective was to evaluate the GenoType NTM-DR assay ability to detect clarithromycin and amikacin resistance in M. abscessus complex isolates compared with drug susceptibility testing (DST) and PCR sequencing of the erm(41), rrl and rrs genes. The concordance between the GenoType NTM-DR and MLST results concerning subspecies identification was 100%. The wild type and mutated alleles of the rrl and rrs genes were detected by the GenoType NTM-DR assay and PCR sequencing with 100% (115/115) agreement. Similarly, 100% concordance between GenoType NTM-DR and DST was observed for clarithromycin and amikacin testing. Sensitivity for the detection of clarithromycin and amikacin resistance was 100%. The GenoType NTM-DR assay provides a robust and complementary tool to the gold standard methods (MLST and broth microdilution) for subspecies identification and drug resistance detection.

Asunto(s)

Antibacterianos/farmacología , Farmacorresistencia Bacteriana/genética , Técnicas de Genotipaje/instrumentación , Pruebas de Sensibilidad Microbiana/instrumentación , Mycobacterium abscessus/genética , Juego de Reactivos para Diagnóstico , Amicacina/farmacología , Amicacina/uso terapéutico , Antibacterianos/uso terapéutico , Claritromicina/farmacología , Claritromicina/uso terapéutico , Análisis Mutacional de ADN/instrumentación , ADN Bacteriano/genética , ADN Bacteriano/aislamiento & purificación , Genes Bacterianos/genética , Humanos , Tipificación de Secuencias Multilocus , Mutación , Infecciones por Mycobacterium no Tuberculosas/tratamiento farmacológico , Infecciones por Mycobacterium no Tuberculosas/microbiología , Mycobacterium abscessus/aislamiento & purificación , Reacción en Cadena de la Polimerasa