RESUMEN
For adherently growing cells, cultivation is limited by the provided growth surface. Excellent surface-to-volume ratios are found in highly porous matrices, which have to face the challenge of nutrient supply inside the matrices' caverns. Therefore, perfusion strategies are recommended which often have to deal with the need of developing an encompassing bioreactor periphery. We present a modular bioreactor system based on a porous ceramic matrix that enables the supply of cells with oxygen and nutrients by perfusion. The present version of the reactor system focuses on simple testing of various inoculation and operation modes. Moreover, it can be used to efficiently test different foam structures. Protocols are given to set-up the system together with handling procedures for long-time cultivation of a CHO cell line. Experimental results confirm vital growth of cells inside the matrices' caverns.
Asunto(s)
Óxido de Aluminio/química , Reactores Biológicos , Técnicas de Cultivo de Célula/instrumentación , Cerámica/química , Animales , Células CHO , Adhesión Celular , Técnicas de Cultivo de Célula/métodos , Proliferación Celular , Cricetinae , Cricetulus , Diseño de Equipo , Etidio/química , Fluoresceínas/química , Colorantes Fluorescentes/química , Glucosa , Lactosa , Microscopía Electrónica de Rastreo , PorosidadRESUMEN
Porous ceramics made of alumina and hydroxyapatite were created using a protein foaming method. Porosity and pore size distribution were successfully varied by means of chemical modification of the foaming protein Bovine serum albumin (BSA). The effectiveness of the BSA and of its chemical modifications as well as the influence of the dispersing agent were investigated using synchrotron tomography. Resulting porous ceramic materials were used as three-dimensional substrates for the cultivation of human peripheral stem cells. The cells proliferated and differentiated in culture. Five cell lines consistent with human blood cell lines were observed.