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Minerva Med ; 71(6): 401-4, 1980 Feb 25.
Artículo en Italiano | MEDLINE | ID: mdl-6928510

RESUMEN

A new enzymatic fluorimetric assay for the determination of total serum bile acids is described. The enzyme 3 alpha-idroxysteroid NAD oxireductase by oxydising the free OH in position 3 alpha of bile acid molecule, reduce NAD previously added. Afterwards the hydrogen of the generated NADH is transferred by a diaphorase to resazurin to yield the fluorophore resorufin proportionally to the amount of bile acids in the sample. The assay is simple, accurate, without bilirubin's influence and useful for a routine use because the analysis time is not different from the more common laboratory tests.


Asunto(s)
3-Hidroxiesteroide Deshidrogenasas , Ácidos y Sales Biliares/sangre , Espectrometría de Fluorescencia , 3-alfa-Hidroxiesteroide Deshidrogenasa (B-Específica) , Adulto , Ácidos y Sales Biliares/análisis , Femenino , Humanos , Masculino
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